LOCUS       sequence-19941-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB
  TITLE     Nanosensor detection of an immunoregulatory tryptophan 
            influx/kynurenine efflux cycle.
  JOURNAL   PLoS Biol. 2007 Oct;5(10):e257.
  PUBMED    17896864
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2007-10"; volume: "5(10)"; pages: "e257"
COMMENT     Sequence Label: pTK204
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..112
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(116..124,125..139)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(197..218)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(458..477)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     gap             612..622
                     /estimated_length=11
ORIGIN
        1 canattctnt anaatatttt gtttacttta agaaggagat atacatatgc ggggttctca
       61 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      121 gtacgacgat gacgataagg atccgggccg catggtgagc aagggcgagg agctgttcac
      181 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      241 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      301 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      361 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      421 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      481 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      541 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccatca
      601 aannannncg gnnnnnnnnn nnanacggtc agcattnnnc gcnanttaga ngactgatac
      661 gactgatacc acatacacaa caccctcgca cgcctgtgtg cgaacatnta cacatcgcta
      721 caaccacaac catntgctnt gatcaccccg tctttcattt aaagtncacc ttcctnganc
      781 cngggntntt ttttntnnaa acctg
//
