LOCUS       sequence-199410-       10155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 3 - GAL4 synthetic promoter (with eight binding sites) drives
            the expression of STE transcripts containing miR1-Bs(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN472 (FuGW-G8p-STE-1Pe)
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             782..808
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1544..1564
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    2648..3236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2701..2721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3119..3130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3239..3255)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3240..3256)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3761..3941
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3967..3984)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3973..4197
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4243..4671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4330..4349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4540..4561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4680..4700)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4685..5014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4865..5000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4927..4946
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5062..5109
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5128..5502
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5632..5753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5669..5688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5723..5742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5802..5818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5802..5818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5815..5837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5826..5842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5850..5856,5857..5874,5875..5880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5895..5916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6033..6050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6204..6792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6284..6303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6963..7754,7755..7823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7586..7605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7824..7928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8003..8022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8194..8573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8575..8773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8728..8748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8791..8971
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9018..9143
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9636..9869
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10054..10098
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      241 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      301 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      361 ggtttattac agggacagca gagatccagt ttggttaatg gatcctcgat aggtaccgag
      421 tttctagacg gagtactgtc ctccgagcgg agtactgtcc tccgactcga gcggagtact
      481 gtcctccgat cggagtactg tcctccgcga attccggagt actgtcctcc gaagacgcta
      541 gacggagtac tgtcctccga gcggagtact gtcctccgac tcgagcggag tactgtcctc
      601 cgaagacgct agcggggggc tataaaaggg ggtgggggcg ttcgtcctca ctctagatct
      661 gcgatctaag taagcttggc attccggtac tgttggtaaa gccgccacca tgcggctctg
      721 catcccgcag gtgctgttgg ccttgttcct ttccatgctg acagggccgg gagaaggcag
      781 ctacccttac gatgtacctg attacgccgg cggcggaggc tcacaagtgc agcttcagca
      841 gagcggcgct gagctggccc ggcccggggc ctctgtgaaa atgagctgca aggcttccgg
      901 ttacaccttt acaaggtaca caatgcactg ggtgaagcag cggccagggc aggggctcga
      961 gtggatcggc tacataaacc catctcgggg atatacaaac tacaatcaaa agttcaaaga
     1021 caaggccaca ctgacaacgg acaagtcaag cagcacagct tacatgcaac tgagctctct
     1081 gacatccgag gatagcgccg tttactattg cgccagatac tatgatgacc actattgctt
     1141 ggactactgg ggacagggaa caaccctcac agttagctcc ggcggggggg gcagtggagg
     1201 tggaggatct gggggcggcg gtagtcagat cgtcctcaca cagtctccgg ccataatgtc
     1261 cgcctccccc ggagagaagg ttactatgac atgttccgca tcttcctctg tgtcatatat
     1321 gaattggtac cagcagaaga gtggcacctc tcctaaacgc tggatttacg atacctctaa
     1381 actggcgtcc ggggtgcctg cacatttccg cggatccggc tccggtacga gttattcact
     1441 cacaatatct ggaatggagg ccgaagatgc cgctacttac tactgccaac aatggtcaag
     1501 caaccccttc actttcggga gcgggacaaa gctggagatc aaccaccatc atcatcacca
     1561 tcatgcaggt atggcctcct ctgggtatgt cctccaggcg gagctctccc cctcaactga
     1621 gaactcaagt cagctggact tcgaagatgt atggaattct tcctatggtg tgaatgattc
     1681 cttcccagat ggagactatg atgccaacct ggaagcagct gccccctgcc actcctgtaa
     1741 cctgctggat gactctgcac tgcccttctt catcctcacc agtgtcctgg gtatcctagc
     1801 tagcagcact gtcctcttca tgcttttcag acctctcttc cgctggcagc tctgccctgg
     1861 ctggcctgtc ctggcacagc tggctgtggg cagtgccctc ttcagcattg tggtgcccgt
     1921 cttggcccca gggctaggta gcactcgcag ctctgccctg tgtagcctgg gctactgtgt
     1981 ctggtatggc tcagcctttg cccaggcttt gctgctaggg tgccatgcct ccctgggcca
     2041 cagactgggt gcaggccagg tcccaggcct caccctgggg ctcactgtgg gaatttgggg
     2101 agtggctgcc ctactgacac tgcctgtcac cctggccagt ggtgcttctg gtggactctg
     2161 caccctgata tacagcacgg agctgaaggc tttgcaggcc acacacactg tagcctgtct
     2221 tgccatcttt gtcttgttgc cattgggttt gtttggagcc aaggggctga agaaggcatt
     2281 gggtatgggg ccaggcccct ggatgaatat cctgtgggcc tggtttattt tctggtggcc
     2341 tcatggggtg gttctaggac tggatttcct ggtgaggtcc aagctgttgc tgttgtcaac
     2401 atgtctggcc cagcaggctc tggacctgct gctgaacctg gcagaagccc tggcaatttt
     2461 gcactgtgtg gctacgcccc tgctcctcgc cctattctgc caccaggcca cccgcaccct
     2521 cttgccctct ctgcccctcc ctgaaggatg gtcttctcat ctggacaccc ttggaagcaa
     2581 atcctaaccg gtgcaccgct tgaagtcttt aattaaatac accggtgtcg atatcaagct
     2641 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2701 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2761 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2821 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2881 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2941 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3001 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3061 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3121 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3181 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3241 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     3301 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     3361 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3421 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     3481 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     3541 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     3601 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     3661 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     3721 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     3781 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3841 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3901 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     3961 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4021 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4081 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4141 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4201 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4261 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4321 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4381 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4441 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4501 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4561 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4621 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4681 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4741 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4921 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4981 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5041 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     5101 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     5161 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     5221 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     5281 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     5341 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     5401 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     5461 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     5521 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5581 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5761 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5821 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5881 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5941 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6001 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7921 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     7981 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8041 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8101 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     8161 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     8221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     8281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     8341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     8401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     8461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     8521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     8581 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     8641 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     8701 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     8761 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8941 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9001 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9061 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9121 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9181 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9241 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9301 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9361 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9421 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9481 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9541 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9601 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9661 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9721 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9781 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9841 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9901 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9961 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    10021 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    10081 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    10141 ttattcataa tgata
//
