LOCUS       sequence-199412-        9598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 1 - synthetic promoter cMYC drives self-inhibiting GAD 
            expression (see PMID: 29056342 for detailed information).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3
            intr...
FEATURES             Location/Qualifiers
     source          1..9598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             109..120
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     518..538
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     ncRNA           1195..1209
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1266..1378
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    2324..2441
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2739..2783)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2968..3201
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3694..3819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3866..4046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4064..4262)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4089..4109)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4264..4643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4815..4834
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4909..5013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5014..5082,5083..5874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6045..6633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6534..6553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6787..6804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6921..6942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6957..6962,6963..6980,6981..6987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6995..7011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7000..7022
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7019..7035
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7019..7035
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7084..7205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7095..7114)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7149..7168
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7335..7709)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7728..7775)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7823..8152)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7837..7972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7891..7910)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8137..8157
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8166..8594)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8276..8297)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8488..8507
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8640..8864
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8853..8870
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8896..9076
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     9581..9597
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9582..9598
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
       61 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
      121 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
      181 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
      241 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
      301 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
      361 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
      421 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
      481 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
      541 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
      601 cttgatatcg cggccgccct gcacctgcag tccgccacgt gcgcgacagt ccgccacgtg
      661 cgcgactggt ccgccacgtg cgcgaccgtc cgccacgtgc gcgaccggtc cgccacgtgc
      721 gcgaccgtcc gccacgtgcg cgacgtgtcc gccacgtgcg cgccagacgc tagcgggggg
      781 ctataaaagg gggtgggggc gttcgtcctc actctagatc tgcgatctaa gtaagcttgg
      841 cattccggta ctgttggtaa aggatccatg aagctactgt cttctatcga acaagcatgc
      901 gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt
      961 ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtaagt gtgctcgctt
     1021 cggcagcaca tatactatgt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
     1081 atcttggaaa cacttgctgg gattacttct tcagggactt cttaacccaa cagaaggctc
     1141 gagaaggtat attgctgttg acagtgagcg ccgcttgaag tctttaatta aatagtgaag
     1201 ccacagatgt atttaattaa agacttcaag cggtgcctac tgcctcggac ttcaaggggc
     1261 tagaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc
     1321 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
     1381 tcaattgtac taacttcgag tcttcttttt ttttttcaca ggtctccgct gactagggca
     1441 catctgacag aagtggaatc aaggctagaa agactggaac agctatttct actgattttt
     1501 cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg
     1561 ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct
     1621 tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca
     1681 tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcggaatt ctcgacggcc
     1741 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
     1801 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     1861 ggtccgggat gaaagcttca taggatggca agatcctggt atcggtctgc gaccgcttga
     1921 agtctttaat taaaccgctt gaagtcttta attaaaccgc ttgaagtctt taattaaacc
     1981 gcttgaagtc tttattaaac ctctggccac atcggttcct gctccgcttg aagtctttaa
     2041 ttaaaccgct tgaagtcttt aattaaaccg cttgaagtct ttaattaaac cgcttgaagt
     2101 ctttaattaa acctctggcc acatcggttc ctgctccgct tgaagtcttt aattaaaccg
     2161 cttgaagtct ttaattaaac cgcttgaagt ctttaattaa accgcttgaa gtctttaatt
     2221 aaacccgggt aagcgggact ctggggttcg aaatgaccgg tcaaataaaa tacgaaatgt
     2281 taattaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     2341 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     2401 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     2461 tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct
     2521 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     2581 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     2641 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     2701 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     2761 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     2821 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     2881 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     2941 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     3001 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     3061 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     3121 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     3181 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     3241 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     3301 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg
     3361 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     3421 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     3481 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     3541 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
     3601 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     3661 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     3721 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     3781 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     3841 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     3901 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     3961 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     4021 cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta tatagacctc
     4081 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     4141 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     4201 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     4261 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     4321 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     4381 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     4441 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     4501 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     4561 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     4621 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
     4681 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt
     4741 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata
     4801 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ggggatcggg
     4861 agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6841 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6901 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6961 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7021 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca
     7081 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7141 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7201 aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     7261 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     7321 tctcgtagca cgtgtcagtc ctgctcctcg gccacgaagt gcacgcagtt gccggccggg
     7381 tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg
     7441 gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg
     7501 ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg
     7561 atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg
     7621 gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc
     7681 accggaacgg cactggtcaa cttggccatg gtttagttcc tcaccttgtc gtattatact
     7741 atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg
     7801 atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     7861 acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata aaaaaaatta
     7921 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     7981 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     8041 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     8101 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     8161 gaattaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     8221 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     8281 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     8341 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     8401 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     8461 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     8521 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     8581 gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc
     8641 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
     8701 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
     8761 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
     8821 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
     8881 ggtttaaacg ggccctgcta gagattttcc acactgacta aaagggtctg agggatctct
     8941 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     9001 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     9061 ctaaccagag agacccagta cagtccggat gcagctctcg ggccatgtga tgaaatgcta
     9121 ggcggctgtc aaacctccac tctaatactt ctctctccgg gtcatccatc ccatgcaggc
     9181 tcacagggtg taacaagcgg gtgttctctc cttcattggc ttcttctacc ttctcttgct
     9241 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgatc cctggccctg
     9301 gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
     9361 tatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
     9421 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg
     9481 gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg
     9541 ctgtattgct acttgtgatt gctccatgtt tttctaggtc tcgaggtcga cggtatcg
//
