LOCUS       sequence-199418-       10351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 3 - GAL4 synthetic promoter (with eight binding sites) drives
            the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)
FEATURES             Location/Qualifiers
     source          1..10351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    341..458
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2429..2485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2944..3532
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2997..3017)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3415..3426)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3535..3551)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3536..3552)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4057..4237
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4263..4280)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4269..4493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4539..4967
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4626..4645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4836..4857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4976..4996)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4981..5310
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5161..5296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5223..5242
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5358..5405
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5424..5798
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5928..6049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5965..5984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6019..6038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6098..6114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6098..6114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6111..6133)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6122..6138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6146..6152,6153..6170,6171..6176))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6191..6212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6329..6346)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6500..7088)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6580..6599)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7259..8050,8051..8119))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7882..7901
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8120..8224)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8299..8318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8490..8869
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8871..9069
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9024..9044
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9087..9267
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9314..9439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9932..10165
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(10350..10351,1..43)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
       61 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      121 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      181 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      241 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
      301 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      361 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      421 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      481 gagatccagt ttggttaatc ctgcaggcga taggtaccga gtttctagac ggagtactgt
      541 cctccgagcg gagtactgtc ctccgactcg agcggagtac tgtcctccga tcggagtact
      601 gtcctccgcg aattccggag tactgtcctc cgaagacgct agacggagta ctgtcctccg
      661 agcggagtac tgtcctccga ctcgagcgga gtactgtcct ccggctagcg gggggctata
      721 aaagggggtg ggggcgttcg tcctcactct agatctgcga tctaagtaag cttggcattc
      781 cggtactggg cgcgccacca tgtgtcacca acagttggtt atctcttggt tcagtcttgt
      841 cttcctggct tcccccctgg tagccatctg ggagttgaag aaagatgtgt acgtggtgga
      901 attggactgg tatccggacg cacccggtga gatggtggta cttacctgcg acacccccga
      961 ggaggacgga atcacctgga ctcttgatca gtctagcgag gtcttgggta gtggaaagac
     1021 cctgaccatt caggtgaagg aattcggaga tgcagggcag tacacatgtc acaagggtgg
     1081 ggaagtgctc tctcattccc ttcttctcct ccacaaaaag gaggacggaa tttggtcaac
     1141 cgatattctg aaggatcaga aagagccaaa aaacaaaaca ttcctgaggt gtgaagccaa
     1201 gaactacagc ggacggttta cgtgttggtg gctgactaca atttcaaccg atctgacttt
     1261 cagtgtgaag tcctcccgcg gcagctcaga tccccagggg gtgacatgtg gcgcagcgac
     1321 gctctccgca gagcgagtga ggggggataa caaagaatac gaatatagcg tggagtgcca
     1381 ggaggattcc gcctgcccag ccgccgaaga gtccctgccc atcgaggtta tggtcgacgc
     1441 agtgcataaa ctcaaatacg agaactatac gtcctccttt ttcatacgcg acatcatcaa
     1501 acctgacccc ccaaaaaacc ttcaactcaa acccctgaaa aatagtcggc aggtggaggt
     1561 cagttgggaa tacccggata cttggagtac tccgcactcc tatttttcct tgactttttg
     1621 tgtccaggta cagggaaaga gcaaaaggga aaagaaggac cgcgtcttca ctgacaagac
     1681 atcagctaca gtgatctgtc gaaagaatgc aagcattagc gtgagagccc aggacaggta
     1741 ctatagttct agctggtcag aatgggcaag cgtaccttgc tctggcggtg gaggctccgg
     1801 cggggggggg agcggcgggg gaggatctcg gaatctccct gttgccactc cagacccagg
     1861 tatgtttcca tgcctgcacc actctcagaa cttgctccgg gctgtctcta acatgctgca
     1921 gaaggccagg cagacattgg agttttaccc ctgtacgtct gaagagatcg atcatgagga
     1981 cattacaaag gataaaacat caacggtcga agcctgcctt ccccttgagc ttaccaaaaa
     2041 tgaaagctgt ctgaatagcc gcgaaacatc ctttattact aatggttcat gcctcgcctc
     2101 caggaaaaca tcttttatga tggcgctctg tctcagttct atttatgagg atctcaaaat
     2161 gtatcaagtt gagttcaaga ccatgaacgc caaactgctg atggatccta aacgccagat
     2221 ctttttggat caaaatatgc ttgctgtcat cgacgaactc atgcaggccc tgaatttcaa
     2281 ctccgagacc gttccccaaa aatcatccct ggaagagccc gacttttaca aaacaaagat
     2341 caagctctgc attcttctgc acgcttttcg gatccgagct gtgactatag acagagtgat
     2401 gtcctacctg aatgctagtg gaagcggagc tactaacttc agcctgctga agcaggctgg
     2461 agacgtggag gagaaccctg gacctgctca atccttggcg ctgtccttgc tgatcttggt
     2521 gctggcattt ggcattcccc gcacacaagg aagcgatggt ggcgcacagg actgttgcct
     2581 gaagtattca cagcggaaaa tcccagctaa agtggttcgg agctatcgca agcaggaacc
     2641 ttccttgggg tgttcaatcc cagctatcct ctttcttcca agaaagcgct ctcaggccga
     2701 gctgtgtgct gacccaaaag agctgtgggt ccagcagttg atgcagcact tggacaagac
     2761 gccgtctccc cagaaacccg cccagggctg cagaaaagat aggggcgcct caaaaacagg
     2821 caaaaaaggg aaaggtagca aaggttgcaa acgcaccgaa cgaagccaga cgccaaaagg
     2881 gccataaccg gtgcaccgct tgaagtcttt aattaaatac cggtcgatat caagcttatc
     2941 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3001 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3061 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3121 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3181 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3241 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3301 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3361 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3421 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3481 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3541 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     3601 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     3661 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3721 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     3781 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     3841 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     3901 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     3961 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     4021 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     4081 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4141 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4201 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     4261 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4321 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4381 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4441 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4501 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4561 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4621 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4681 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4741 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4801 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4861 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4921 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4981 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5041 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5101 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5161 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5221 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5281 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5341 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     5401 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     5461 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     5521 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     5581 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     5641 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     5701 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     5761 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     5821 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5881 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6061 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6121 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6181 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6241 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6301 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6361 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6421 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6481 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6541 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6601 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6661 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6721 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6781 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6841 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6901 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6961 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7021 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7081 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7141 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7201 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7261 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7321 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7381 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7441 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7501 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7561 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7621 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7681 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7741 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7801 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7861 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7921 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7981 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8041 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8101 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8161 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8221 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8281 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8341 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8401 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8461 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8521 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8581 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8641 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8701 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8761 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8821 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     8881 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     8941 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9001 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9061 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9121 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9181 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9241 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9301 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9361 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9421 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9481 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9541 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9601 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9661 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9721 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9781 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9841 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9901 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9961 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    10021 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10081 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10141 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10201 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10261 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10321 taattgaaga atcgcaaaac cagcaagaaa a
//
