LOCUS       sequence-199419-        9558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 1 - synthetic promoter USF1 drives self-inhibiting GAD 
            expression (see PMID: 29056342 for detailed information).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3
            intr...
FEATURES             Location/Qualifiers
     source          1..9558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..44
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     29..45
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    48..636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             154..165
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     563..583
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     ncRNA           1200..1214
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1271..1383
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    2329..2446
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2744..2788)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2973..3206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3699..3824
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3871..4051
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4069..4267)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4094..4114)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4269..4648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4820..4839
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4914..5018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5019..5087,5088..5879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5237..5256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6050..6638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6539..6558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6792..6809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6926..6947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6962..6967,6968..6985,6986..6992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7000..7016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7005..7027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7024..7040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7024..7040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7089..7210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7100..7119)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7154..7173
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7340..7714)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7733..7780)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7828..8157)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7842..7977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7896..7915)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8142..8162
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8171..8599)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8281..8302)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8493..8512
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8645..8869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8858..8875
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8901..9081
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgtgattgct ccatgttttt ctaggtctcg aggtcgacgg tatcgatgcg gggaggcggc
       61 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc
      121 cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa
      181 ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga
      241 tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg
      301 ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc
      361 ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac
      421 agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa
      481 ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa
      541 aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt
      601 caatctttca caaattttgt aatccagagg ttgattatcg ataagcttga tatcgcggcc
      661 gccctgcacc tgcaggccac gtgcagacca cgtgctcgcc acgtgcgacc cacgtgccta
      721 ccacgtgcac tccacgtgct gcccacgtgc gtaccacgtg cggcgcgcca gacgctagcg
      781 gggggctata aaagggggtg ggggcgttcg tcctcactct agatctgcga tctaagtaag
      841 cttggcattc cggtactgtt ggtaaaggat ccatgaagct actgtcttct atcgaacaag
      901 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
      961 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg taagtgtgct
     1021 cgcttcggca gcacatatac tatgttgaat gaggcttcag tactttacag aatcgttgcc
     1081 tgcacatctt ggaaacactt gctgggatta cttcttcagg gacttcttaa cccaacagaa
     1141 ggctcgagaa ggtatattgc tgttgacagt gagcgccgct tgaagtcttt aattaaatag
     1201 tgaagccaca gatgtattta attaaagact tcaagcggtg cctactgcct cggacttcaa
     1261 ggggctagaa ttcaaggggc tactttagga gcaattatct tgtttactaa aactgaatac
     1321 cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac
     1381 ttttttcaat tgtactaact tcgagtcttc tttttttttt tcacaggtct ccgctgacta
     1441 gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga
     1501 tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag
     1561 cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat
     1621 tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat
     1681 catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg gaattctcga
     1741 cggccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg
     1801 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
     1861 ccccgggtcc gggatgaaag cttcatagga tggcaagatc ctggtatcgg tctgcgaccg
     1921 cttgaagtct ttaattaaac cgcttgaagt ctttaattaa accgcttgaa gtctttaatt
     1981 aaaccgcttg aagtctttat taaacctctg gccacatcgg ttcctgctcc gcttgaagtc
     2041 tttaattaaa ccgcttgaag tctttaatta aaccgcttga agtctttaat taaaccgctt
     2101 gaagtcttta attaaacctc tggccacatc ggttcctgct ccgcttgaag tctttaatta
     2161 aaccgcttga agtctttaat taaaccgctt gaagtcttta attaaaccgc ttgaagtctt
     2221 taattaaacc cgggtaagcg ggactctggg gttcgaaatg accggtcaaa taaaatacga
     2281 aatgttaatt aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     2341 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     2401 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     2461 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     2521 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     2581 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     2641 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     2701 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     2761 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     2821 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     2881 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     2941 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     3001 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     3061 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     3121 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     3181 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     3241 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     3301 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     3361 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     3421 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     3481 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     3541 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     3601 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     3661 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     3721 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     3781 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     3841 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     3901 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     3961 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     4021 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
     4081 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     4141 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     4201 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     4261 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     4321 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     4381 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     4441 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     4501 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     4561 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     4621 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     4681 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     4741 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     4801 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     4861 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7021 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     7081 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7201 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     7261 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     7321 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     7381 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     7441 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     7501 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     7561 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     7621 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     7681 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
     7741 atactatgcc gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa
     7801 aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     7861 tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa
     7921 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7981 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     8041 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     8101 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     8161 ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     8221 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     8281 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     8341 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     8401 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     8461 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     8521 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     8581 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca
     8641 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     8701 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     8761 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     8821 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     8881 cagcgggttt aaacgggccc tgctagagat tttccacact gactaaaagg gtctgaggga
     8941 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     9001 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     9061 ctggtctaac cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa
     9121 tgctaggcgg ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg
     9181 caggctcaca gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc
     9241 ttgctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg
     9301 ccctggtgtg tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     9361 aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     9421 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     9481 gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt
     9541 agctgctgta ttgctact
//
