LOCUS       sequence-199428-        9405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length human b2 with tension sensor at b2_733.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Nordenfelt P, Elliott HL, Springer TA
  TITLE     Coordinated integrin activation by actin-dependent force during 
            T-cell migration.
  JOURNAL   Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.
  PUBMED    27721490
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-10"; volume: "7:13119. doi"; pages: " 
            10.1038/ncomms13119"
COMMENT     Sequence Label: b2-TS3
FEATURES             Location/Qualifiers
     source          1..9405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(27..734)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(2970..2989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2971..2989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3034..3237)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3063..3083)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3238..3617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3789..3808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3883..3987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3988..4056,4057..4848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4206..4225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5019..5607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5508..5527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5761..5778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5895..5916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5931..5936,5937..5954,5955..5961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5969..5985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5974..5996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5993..6009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5993..6009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6058..6179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6069..6088)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6123..6142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6309..7334)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6309..7334)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7383..7712)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7397..7532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7451..7470)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7697..7717
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7726..8154)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7836..7857)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8048..8067
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8200..8424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8413..8430
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(8595..9305,9306..9308,9309..9311))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8637..8658)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8986..9005
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9245..9266
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccacctggcc ctgcaccacc tggccccttg tacagctcgt ccatgccgtc ggtggagttg
       61 cgggccacgg cgctctcgta aacggtcacc ttgttgtagt ccttgtcgtg gttcaggatc
      121 tcgatgcggt ggtccacaaa gtgatagtcg ggcagcttca ccgccttctt ggccctgtag
      181 atggtcttga agtcaacgcg gtggtggccg ccgccctcca gcagcagctt gtgcttgacg
      241 tcgcccttca gcacgccgtc gcgcacgtac atcctctcgg tggaggcgtc ccagccggtg
      301 gtcttcttct gcatcacggg gccgttgggg gggaagttct cgcccttgag gtgtatctcg
      361 tagatgaagg agtcctcctc catggagatg tcggacttca ccttcacgat gcccttgtcc
      421 tcgaaggtca tggtgcgctc ccaagagtag ccctcgggga aggactgctt gaagtagttg
      481 gggatgtcgt cggggtactt ggtgaaggcc ctgttgccgt aggcgaacgc ggtggtcaga
      541 atgtcgtagg agaagggcag gggggctccc tccttcacct ccaggttgat ggtgttggtg
      601 ccgtcgtagg gcttgccctc gccctcgccc tcgatcacga aggcgtggcc attcacgttg
      661 ccctccatct tcagcttgat cttcatgtcg ggcttgatta cgcccattgt ggtctcctcg
      721 cccttgctca ccatcgcctg agcctgccgg aggtcgctca ggtggatcag agccttccag
      781 atgaccagca ggagaatgcc gatcagcacg atgcctgcca cggtgccccc gacgatggcg
      841 gcgatgttgg ggcctgccac acactctcgg ctctcatcca catagatgag gtagcggtcc
      901 atcccgtcct gctgctccag cgtgtaggcc acccagcagc cctctgagtc cctctccttg
      961 caggtcctgc ccttcacggg gttgttcgac agctgcaggc ccggacacgc cgcgctgcag
     1021 ttcttcccaa aggggccctt ttcgaacttc aggcactcgg cgcaggagat gtacttgcca
     1081 cagggtgagg ggcagccggg gcactcctgg cacagaggca gctggtagcc tgaatggcac
     1141 tcgcatacgt tgcagcggca ccggccacga ccactacact caacacgccg cgggttcagg
     1201 cagccctcag tggtcctctc gcactggcac gctgagccct caaagcccgg gtggcagcgg
     1261 cacttcccgc agaagcagag ccccctcccc gggccgccgc agacctggcc gttgtagcgc
     1321 tcacagttga tggtgtcaca ctcgcagtac tgcccgtata tcagcttgcc ggggacgtcg
     1381 ctggtgtggc acaggcactg cccgcagaca cagtccccca gccctgagca gatgatggag
     1441 ttgttgtcct tccggcagct tccttccagc tcctggctgc tccggccctg tgtctggcac
     1501 tcacagtttt tcccaatgta gccagtgtca cacctgcaga tgccgcactc caagaagccc
     1561 ttgccatggc agaggctgcg gtctctgctc tggtcccggc accggcactc acactgggga
     1621 agaacctgca cggtcactat gtccgtgaag cccagcgccc ggatgacaaa cgactgctcc
     1681 tggatgcact ctgtggccgt gaccttcacc tggaaggtga tcgggacatt gatctgcacg
     1741 ccatcacagt cacctctggg ctggttcctg tgcgtcactc cattgctgca gaaggagtcg
     1801 taggtgactt tcagggtgtc ggggagggcg ttgtgatcca ggaagaccct ggaggagagt
     1861 ttattgtaag cattcttaat gagatggacc acattgctgg agtcctcaga cagctccccc
     1921 acggctgact tggggatgat ctcggtgagt ttctcgtagg tcttcaccat cctactggtc
     1981 accgcgaaga tgggctggat gttgttttca gccagcttgt gcgccagctg gcccaccgat
     2041 gggtagtcga attcgttgct cctcttgtac aagttgtcct ccaggtgaca gcggccgtcg
     2101 ttgggggtca ggatggcgcc cagctttccg tcgcccgcga aatggaagcc gtcatcagtg
     2161 gcaaacacca gcagccgcgt gacgttgcgc cagccgattt cctccgggca ggcggcgacc
     2221 tgcatcatgg cgtccagccc accctcgggt gcatccaggt ttccggaaat cagctgcttc
     2281 ccgacctcgg tctgaaactg gttggagttg ttggtcagct tcagcacgtg cctgaaggca
     2341 aacgggggct ggcactcttt ctccttgttg gggcatgggt ttcgcagctt atcagggtgc
     2401 gtgttcacga acggcagcac ggtcttgtcc acgaaggacc cgaagccaat gcggccggac
     2461 tcggtgatct cgttgagggc ccggagcagg tcgccaccta gcttcttgac attcctgagg
     2521 tcatcaagca tggagtagga gaggtccatc agatagtaca ggtcgatggg gtagcccttg
     2581 gcccgccgga aggtcacgtt gaacgctgct gcctggcctg gtcgcaggta aagcgtcact
     2641 ttttgtgggg acagctgctt ctggccccca ttgtggtctt cctgggtttc agcgaggctt
     2701 gtggggtcca tgatgtcgtc agccgcacag cccctcatga gcagctgtgg ccgggtgtcg
     2761 cagcgaatgg agtcaggatc ccccggccct gtgaagttca gcttctggca ccaggtgcag
     2821 ccgggccccg actcgatgca ttcccggcag ctgctgacct tgaacttcgt gcactcctga
     2881 gagaggacgc acccgaggga gagcagcccc accagggcga gcagtggggg gcgcaggccc
     2941 agcatggtgg cgctagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag
     3001 ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg
     3061 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     3121 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     3181 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     3241 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     3301 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     3361 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     3421 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     3481 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     3541 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     3601 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     3661 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     3721 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     3781 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     3841 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3901 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3961 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4021 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4081 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4141 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4201 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4261 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4321 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4381 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4441 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4501 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4561 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4621 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4681 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4741 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4801 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4861 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5041 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5101 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5161 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5221 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5281 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5341 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5401 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5581 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5641 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5701 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5761 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5821 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5881 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     5941 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6001 agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa
     6061 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     6181 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     6241 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     6301 agcacgtgct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     6361 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     6421 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     6481 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     6541 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     6601 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     6661 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     6721 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     6781 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     6841 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     6901 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     6961 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     7021 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
     7081 tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     7141 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     7201 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     7261 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     7321 tcaggctttt tcatcacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc
     7381 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     7441 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     7501 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     7561 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7621 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     7681 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     7741 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7801 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7861 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7921 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7981 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     8041 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     8101 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     8161 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     8221 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     8281 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     8341 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     8401 atggctggca actagaaggc acagtcgagg ctgatcagcg gtttaaactt aagcttttac
     8461 taactctcag caaacttggg gttcatgacc gtcgtggtgg cgctcttgaa aaggggatta
     8521 tcattgttcc actgggactt gagcttctcc ttctcaaagc gcctgtactc ccggaggtcg
     8581 ctttgtgctt gagccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     8641 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag tttggactgg
     8701 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     8761 tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg
     8821 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct gttgtagttg
     8881 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     8941 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     9001 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     9061 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagcccagg
     9121 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat cagcttcagg
     9181 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     9241 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     9301 ttgctcacca tagcaccacc tggacctgca ccacctggac ctgcaccacc tggacctgca
     9361 ccaccgggcc ctgcaccacc tggccctgca ccacctggcc ctgca
//
