LOCUS       sequence-199430-        9405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length human b2 with tension sensor at b2_743.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Nordenfelt P, Elliott HL, Springer TA
  TITLE     Coordinated integrin activation by actin-dependent force during 
            T-cell migration.
  JOURNAL   Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.
  PUBMED    27721490
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-10"; volume: "7:13119. doi"; pages: " 
            10.1038/ncomms13119"
COMMENT     Sequence Label: b2-TS5
FEATURES             Location/Qualifiers
     source          1..9405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..35
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(154..174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        159..488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      339..474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     401..420
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             537..1562
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             537..1562
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1692..1813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1729..1748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1783..1802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1862..1878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1862..1878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1875..1897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1886..1902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1910..1916,1917..1934,1935..1940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1955..1976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2093..2110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2264..2852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2344..2363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3023..3814,3815..3883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3646..3665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3884..3988)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4063..4082)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4254..4633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4634..4837
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4788..4808
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4882..4901
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4882..4900
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7167..7874
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(7995..7997,7998..8000,8001..8711)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8040..8061)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8301..8320)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8648..8669
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8846..8863)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8852..9076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9122..9405,1..145)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9209..9228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
       61 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      121 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
      181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      241 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
      421 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      481 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga
      541 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
      601 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
      661 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
      721 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
      781 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
      841 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
      901 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
      961 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     1021 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     1081 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     1141 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     1201 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     1261 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     1321 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     1381 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     1441 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     1501 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     1561 agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     1621 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1681 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1801 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1861 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1921 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1981 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2041 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2101 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2161 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2821 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2881 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2941 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3001 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3061 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3121 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3181 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3241 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3301 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3361 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3421 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3481 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3541 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3601 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3661 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3721 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3781 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3841 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3901 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3961 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     4021 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     4081 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     4141 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     4201 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     4261 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4321 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4381 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4441 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4501 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4561 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4621 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4681 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4741 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     4801 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     4861 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg
     4921 ccaccatgct gggcctgcgc cccccactgc tcgccctggt ggggctgctc tccctcgggt
     4981 gcgtcctctc tcaggagtgc acgaagttca aggtcagcag ctgccgggaa tgcatcgagt
     5041 cggggcccgg ctgcacctgg tgccagaagc tgaacttcac agggccgggg gatcctgact
     5101 ccattcgctg cgacacccgg ccacagctgc tcatgagggg ctgtgcggct gacgacatca
     5161 tggaccccac aagcctcgct gaaacccagg aagaccacaa tgggggccag aagcagctgt
     5221 ccccacaaaa agtgacgctt tacctgcgac caggccaggc agcagcgttc aacgtgacct
     5281 tccggcgggc caagggctac cccatcgacc tgtactatct gatggacctc tcctactcca
     5341 tgcttgatga cctcaggaat gtcaagaagc taggtggcga cctgctccgg gccctcaacg
     5401 agatcaccga gtccggccgc attggcttcg ggtccttcgt ggacaagacc gtgctgccgt
     5461 tcgtgaacac gcaccctgat aagctgcgaa acccatgccc caacaaggag aaagagtgcc
     5521 agcccccgtt tgccttcagg cacgtgctga agctgaccaa caactccaac cagtttcaga
     5581 ccgaggtcgg gaagcagctg atttccggaa acctggatgc acccgagggt gggctggacg
     5641 ccatgatgca ggtcgccgcc tgcccggagg aaatcggctg gcgcaacgtc acgcggctgc
     5701 tggtgtttgc cactgatgac ggcttccatt tcgcgggcga cggaaagctg ggcgccatcc
     5761 tgacccccaa cgacggccgc tgtcacctgg aggacaactt gtacaagagg agcaacgaat
     5821 tcgactaccc atcggtgggc cagctggcgc acaagctggc tgaaaacaac atccagccca
     5881 tcttcgcggt gaccagtagg atggtgaaga cctacgagaa actcaccgag atcatcccca
     5941 agtcagccgt gggggagctg tctgaggact ccagcaatgt ggtccatctc attaagaatg
     6001 cttacaataa actctcctcc agggtcttcc tggatcacaa cgccctcccc gacaccctga
     6061 aagtcaccta cgactccttc tgcagcaatg gagtgacgca caggaaccag cccagaggtg
     6121 actgtgatgg cgtgcagatc aatgtcccga tcaccttcca ggtgaaggtc acggccacag
     6181 agtgcatcca ggagcagtcg tttgtcatcc gggcgctggg cttcacggac atagtgaccg
     6241 tgcaggttct tccccagtgt gagtgccggt gccgggacca gagcagagac cgcagcctct
     6301 gccatggcaa gggcttcttg gagtgcggca tctgcaggtg tgacactggc tacattggga
     6361 aaaactgtga gtgccagaca cagggccgga gcagccagga gctggaagga agctgccgga
     6421 aggacaacaa ctccatcatc tgctcagggc tgggggactg tgtctgcggg cagtgcctgt
     6481 gccacaccag cgacgtcccc ggcaagctga tatacgggca gtactgcgag tgtgacacca
     6541 tcaactgtga gcgctacaac ggccaggtct gcggcggccc ggggaggggg ctctgcttct
     6601 gcgggaagtg ccgctgccac ccgggctttg agggctcagc gtgccagtgc gagaggacca
     6661 ctgagggctg cctgaacccg cggcgtgttg agtgtagtgg tcgtggccgg tgccgctgca
     6721 acgtatgcga gtgccattca ggctaccagc tgcctctgtg ccaggagtgc cccggctgcc
     6781 cctcaccctg tggcaagtac atctcctgcg ccgagtgcct gaagttcgaa aagggcccct
     6841 ttgggaagaa ctgcagcgcg gcgtgtccgg gcctgcagct gtcgaacaac cccgtgaagg
     6901 gcaggacctg caaggagagg gactcagagg gctgctgggt ggcctacacg ctggagcagc
     6961 aggacgggat ggaccgctac ctcatctatg tggatgagag ccgagagtgt gtggcaggcc
     7021 ccaacatcgc cgccatcgtc gggggcaccg tggcaggcat cgtgctgatc ggcattctcc
     7081 tgctggtcat ctggaaggct ctgatccacc tgagcgacct ccgggagtac aggcgctttg
     7141 agaaggagaa gctccaggct caggcgatgg tgagcaaggg cgaggagacc acaatgggcg
     7201 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
     7261 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
     7321 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
     7381 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
     7441 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
     7501 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
     7561 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
     7621 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
     7681 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
     7741 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
     7801 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
     7861 acgagctgta caaggggcca ggtggtgcag ggccaggtgg tgcagggcca ggtggtgcag
     7921 ggccaggtgg tgcagggccc ggtggtgcag gtccaggtgg tgcaggtcca ggtggtgcag
     7981 gtccaggtgg tgctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     8041 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     8101 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     8161 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     8221 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8281 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     8341 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     8401 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     8461 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     8521 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8581 tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc aacgagaagc
     8641 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8701 agctgtacaa ggctcaagca caaaaggaga agctcaagtc ccagtggaac aatgataatc
     8761 cccttttcaa gagcgccacc acgacggtca tgaaccccaa gtttgctgag agttagtaaa
     8821 agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     9001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     9061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtcc
//
