LOCUS       sequence-199433-        6080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pombe General Purpose Vector - with Sp.leu1 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     Construction of Designer Selectable Marker Deletions with 
            CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized 
            Design of Common Entry Vectors.
  JOURNAL   G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363.
  PUBMED    29321167
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363."
COMMENT     Sequence Label: pYZ191
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             217..597
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    273..380
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     297..313
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     297..313
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(347..363)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(348..364)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(388..407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(389..407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(417..434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(417..433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(426..448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      574..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(661..680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     871..892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3016..3799
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        4175..4279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4280..4348,4349..5140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4498..4517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5311..5899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5800..5819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6053..6070
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
      301 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
      361 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      541 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      601 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      781 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      901 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      961 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     1021 ttacaatttc aactcggtca taaagttgaa cggatgtcgt aaatcaattc catgcttttg
     1081 caacttaata catctttgct tgactataac ataccaaaag aaaaaacatt agagccttaa
     1141 aatttacgct tttgtcaatt gcatttttaa cgataataaa tatgcgacac aattaatcaa
     1201 taaaaattag gtcaaatcaa atgatatgaa tttattctac attaaaccct attacaaaat
     1261 tttttcaagt tcttcaacaa cagccttagt aatatcagcg gtagaagcct cacctcccaa
     1321 atcgcgagta taaagaccac gtccaccaat tgaagtatca tccaagacct tgcgtacggc
     1381 ggcttcgata gcttcagcct ccttaggagc attcaaacca taacgaagga gaagggaagc
     1441 agataaaatt gtaccaacag gattaacaat gcccttgcca gcgatatcgg gagcgctacc
     1501 gtgaatgggc tcaaccaaac aatgaacctt ttcttctgat tttcctacca caccggaaag
     1561 ggaggcagaa ggcaaaaggc ccaagctacc aggaatgaca gaagcctcat ctgaaataat
     1621 gtcaccaaac aagttgtcag tcaaaacaac accgttaagt gtacgagggc tcttgaccaa
     1681 aagcatggct gcggagtcaa tgagctggtt ttttaaggta aggtgaggat attcctcctt
     1741 aaaaatctta gctacagtct tgcgccaaag acgagaagtt gccaaaacat tagctttgtc
     1801 gagtaatgtg acgggagcag gagggttgga agtttcagct aaccaagcag ccaaacgagc
     1861 aatacgagaa acttcttcca aactgtaagg ccaagtgtcc atagcataac ccgatccgtt
     1921 gtcctcagtg cgctcaccaa agtaacaacc tccagtaagt tctcgtacaa cacaaaaatc
     1981 gacaccttca acgatttcag gcttcaaagg gctgtacttg actaaagact tgctggcaaa
     2041 gttgcaaggt cgaaggttgg cccaaacacc catactctta cgaagcttca ataaaccttg
     2101 ctcaggacga caattggggt tggtccattc aggaccacca acggcaccca aaagaacacc
     2161 gtcagcttcc aaacaagcct tcacagtctc gtcagtcaaa ggggttccat aggcatcaat
     2221 agaggcacct ccaatcttgt gttcttcaaa ctccagtttt aactcaggtc gcttcttctc
     2281 aacgactttc aaaacctcca aggcagaagc aacaatttca gggccaatat gatctcctgg
     2341 taagacgacg attttctttg cacacatgtt gttgaagaag ttttgttgtg aaatggtttc
     2401 gtgaaagttt cagaccctac cgcaaaaatg cctggtttcg ggaaactcaa cactgttgca
     2461 ctttttatac tacagattgg gatatcgata atattgcgta aaaaatcctt tttttaaaaa
     2521 gcttgtttac agtaacgtaa atgaccagaa atcagatgaa aatcacaaga aagcaaataa
     2581 ttcacgttaa atcctgatat gtttgatttt gtgatgaaat catggatgtt cataggaatt
     2641 gttgaaattg cgctttttta acgaaatata caagtatcct ggagcttact taattaatta
     2701 atgaatcttt gtttctagat attaaaatag tagcctcaat tatcagcgct ttctacgtta
     2761 gtaaacgaaa tttttaatgt caaaaaaatg tttaatagac agtacgaata tgcattataa
     2821 tgttcataaa taatttgagt atgtgtacga gttgtcttta aacccacaga ggtagaatgt
     2881 atatataaaa ttaataagct aagtgtaata cttaaaaaat acatgtcgat cgactctaga
     2941 ggaaattcga gtctaactcc ttaaccactc ggacatagtg acttatctga cactcattgt
     3001 aaattaatat atatataggc attttgttta gttaaaggta cttaagtaat tagtataaac
     3061 gaaccaattt tataatcagg aagttaagtg aatggtagca catgtcgtaa aaattgtgaa
     3121 tttttattga ataatatttt aaatacaagc ctttctagac taggtatact cataaacata
     3181 tatgagcaaa aggatagagg agattacatt gcatcttcta caaatttatt tattgccctt
     3241 tactgaaaaa ttaaataatg agtactaaat gataaaaagc gctcagtaca agaaagattg
     3301 caaaaatatt gcattcttca tgaattaaag ttgcatataa ggcatattga aagtaatagt
     3361 actaaaacag cagttagcga aaattaatag aattatattc gcaagacaat tgtgacatta
     3421 aattaaaaaa ttgtagaatt tttactatcc tctttaacgc catgagcctt tataaaaagg
     3481 ttaaattagt tttaacattc tttttttgag taagagttta caatttatca aaacctgtgt
     3541 tattatattc attagtttca atttattagc atctagagaa aaatcaattg gcagttacat
     3601 tgttggaatt tatgaagaaa aagaatctac aacggagaat aagttgctga tcgctttccc
     3661 taaaattgta tattttgctg agcttatttt gacatttcgt tgaagttttc tctaattcgc
     3721 attcatttta agtaaaacaa tgagaaataa aattacaaaa aatacaaatt aaaatacaat
     3781 ttttagctat aattatagac gatgcccttg tatcccattc tgtctcgctt gcccctactt
     3841 tttatctttt atataccata atgaacgctg ccgctactaa ccataccccg attttacatt
     3901 tcggactccc aaggacgtac aaaatagaaa actatagaaa aaaataatca gaaaatagca
     3961 tgtcatctct ttgtaaaacg cgtttgcaag aagaaaggaa acaatggaga agagatcatc
     4021 catttgtatg taaattttag taaacttgaa gaaatcacta acaacttctc ttacttaggg
     4081 attctatgca aaaccttgta aatcatctga tggaggactc gatttaatga attggaaggt
     4141 tggatttcca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4321 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4381 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4441 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4501 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4561 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4621 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4681 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4741 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4801 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4861 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4921 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4981 agccggtgag cgtggatctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5041 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5101 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6061 agtgagcgag gaagcggaag
//
