LOCUS       sequence-199435-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length human aL with EGFP at betapropeller.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Nordenfelt P, Moore TI, Mehta SB, Kalappurakkal JM, Swaminathan V, 
            Koga N, Lambert TJ, Baker D, Waters JC, Oldenbourg R, Tani T, Mayor 
            S, Waterman CM, Springer TA
  TITLE     Direction of actin flow dictates integrin LFA-1 orientation during 
            leukocyte migration.
  JOURNAL   Nat Commun. 2017 Dec 11;8(1):2047. doi: 10.1038/s41467-017-01848-y.
  PUBMED    29229906
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-12-11"; volume: "8(1):2047. doi"; pages: " 
            10.1038/s41467-017-01848-y"
COMMENT     Sequence Label: aL-EGFP
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     77..96
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             213..1238
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             213..1238
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1368..1489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1405..1424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1459..1478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1538..1554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1538..1554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1551..1573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1562..1578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1586..1592,1593..1610,1611..1616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1631..1652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1769..1786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1940..2528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2020..2039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2699..3490,3491..3559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3322..3341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3560..3664)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3739..3758)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3930..4309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4310..4513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4558..4577
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4558..4576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4794..4805
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(5945..5947,5948..5950,5951..6661)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5990..6011)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6251..6270)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6598..6619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8947..8964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8953..9177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9223..9651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9310..9329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9520..9541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9660..9680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9665..9830,1..164)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
       61 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      121 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      181 gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt
      241 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
      301 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
      361 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
      421 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
      481 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
      541 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
      601 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
      661 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
      721 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
      781 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
      841 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
      901 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
      961 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     1021 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     1081 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     1141 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     1201 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt
     1261 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     1321 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     1381 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1441 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     1501 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1561 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1621 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1681 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1741 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3661 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     3721 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     3781 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     3841 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     3901 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     3961 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4021 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4081 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     4141 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     4201 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     4261 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     4321 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4381 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4441 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4501 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     4561 tacgactcac tatagggaga cccaagctgg ctagcgttta aacgggccct ctagagccac
     4621 catgaaggat tcctgcatca ctgtgatggc catggcgctg ctgtctgggt tctttttctt
     4681 cgcgccggcc tcgagctaca acctggacgt gcggggcgcg cggagcttct ccccaccgcg
     4741 cgccgggagg cactttggat accgcgtcct gcaggtcgga aacggggtca tcgtgggagc
     4801 tccaggggag gggaacagca caggaagcct ctatcagtgc cagtcgggca caggacactg
     4861 cctgccagtc accctgagag gttccaacta tacctccaag tacttgggaa tgaccttggc
     4921 aacagacccc acagatggaa gcattttggc ctgtgaccct gggctgtctc gaacgtgtga
     4981 ccagaacacc tatctgagtg gcctgtgtta cctcttccgc cagaatctgc agggtcccat
     5041 gctgcagggg cgccctggtt ttcaggaatg tatcaagggc aacgtagacc tggtatttct
     5101 gtttgatggt tcgatgagct tgcagccaga tgaatttcag aaaattctgg acttcatgaa
     5161 ggatgtgatg aagaaactca gcaacacttc gtaccagttt gctgctgttc agttttccac
     5221 aagctacaaa acagaatttg atttctcaga ttatgttaaa tggaaggacc ctgatgctct
     5281 gctgaagcat gtaaagcaca tgttgctgtt gaccaatacc tttggtgcca tcaattatgt
     5341 cgcgacagag gtgttccggg aggagctggg ggcccggcca gatgccacca aagtgcttat
     5401 catcatcacg gatggggagg ccactgacag tggcaacatc gatgcggcca aagacatcat
     5461 ccgctacatc atcgggattg gaaagcattt tcagaccaag gagagtcagg agaccctcca
     5521 caaatttgca tcaaaacccg cgagcgagtt tgtgaaaatt ctggacacat ttgagaagct
     5581 gaaagatcta ttcactgagc tgcagaagaa gatctatgtc attgagggca caagcaaaca
     5641 ggacctgact tccttcaaca tggagctgtc ctccagcggc atcagtgctg acctcagcag
     5701 gggccatgca gtcgtggggg cagtaggagc caaggactgg gctgggggct ttcttgacct
     5761 gaaggcagac ctgcaggatg acacatttat tgggaatgaa ccattgacac cagaagtgag
     5821 agcaggctat ttgggttaca ccgtgacctg gctgccctcc cggcaaaaga cttcgttgct
     5881 ggcctcggga gcccctcgat accagcacat gggccgagtg ctgctgttcc aagagccaca
     5941 gggcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6001 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6061 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6121 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6181 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6241 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6301 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6361 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6421 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6481 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6541 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     6601 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6661 gggaggacac tggagccagg tccagacaat ccatgggacc cagattggct cttatttcgg
     6721 tggggagctg tgtggcgtcg acgtggacca agatggggag acagagctgc tgctgattgg
     6781 tgccccactg ttctatgggg agcagagagg aggccgggtg tttatctacc agagaagaca
     6841 gttggggttt gaagaagtct cagagctgca gggggacccc ggctacccac tcgggcggtt
     6901 tggagaagcc atcactgctc tgacagacat caacggcgat gggctggtag acgtggctgt
     6961 gggggcccct ctggaggagc agggggctgt gtacatcttc aatgggaggc acggggggct
     7021 tagtccccag ccaagtcagc ggatagaagg gacccaagtg ctctcaggaa ttcagtggtt
     7081 tggacgctcc atccatgggg tgaaggacct tgaaggggat ggcttggcag atgtggctgt
     7141 gggggctgag agccagatga tcgtgctgag ctcccggccc gtggtggata tggtcaccct
     7201 gatgtccttc tctccagctg agatcccagt gcatgaagtg gagtgctcct attcaaccag
     7261 taacaagatg aaagaaggag ttaatatcac aatctgtttc cagatcaagt ctctcatccc
     7321 ccagttccaa ggccgcctgg ttgccaatct cacttacact ctgcagctgg atggccaccg
     7381 gaccagaaga cgggggttgt tcccaggagg gagacatgaa ctcagaagga atatagctgt
     7441 caccaccagc atgtcatgca ctgacttctc atttcatttc ccggtatgtg ttcaagacct
     7501 catctccccc atcaatgttt ccctgaattt ctctctttgg gaggaggaag ggacaccgag
     7561 ggaccaaagg gcgcagggca aggacatacc gcccatcctg agaccctccc tgcactcgga
     7621 aacctgggag atcccttttg agaagaactg tggggaggac aagaagtgtg aggcaaactt
     7681 gagagtgtcc ttctctcctg caagatccag agccctgcgt ctaactgctt ttgccagcct
     7741 ctctgtggag ctgagcctga gtaacttgga agaagatgct tactgggtcc agctggacct
     7801 gcacttcccc ccgggactct ccttccgcaa ggtggagatg ctgaagcccc atagccagat
     7861 acctgtgagc tgcgaggagc ttcctgaaga gtccaggctt ctgtccaggg cattatcttg
     7921 caatgtgagc tctcccatct tcaaagcagg ccactcggtt gctctgcaga tgatgtttaa
     7981 tacactggta aacagctcct ggggggactc ggttgaattg cacgccaatg tgacctgtaa
     8041 caatgaggac tcagacctcc tggaggacaa ctcagccact accatcatcc ccatcctgta
     8101 ccccatcaac atcctcatcc aggaccaaga agactccaca ctctatgtca gtttcacccc
     8161 caaaggcccc aagatccacc aagtcaagca catgtaccag gtgaggatcc agccttccat
     8221 ccacgaccac aacataccca ccctggaggc tgtggttggg gtgccacagc ctcccagcga
     8281 ggggcccatc acacaccagt ggagcgtgca gatggagcct cccgtgccct gccactatga
     8341 ggatctggag aggctcccgg atgcagctga gccttgtctc cccggagccc tgttccgctg
     8401 ccctgttgtc ttcaggcagg agatcctcgt ccaagtgatc gggactctgg agctggtggg
     8461 agagatcgag gcctcttcca tgttcagcct ctgcagctcc ctctccatct ccttcaacag
     8521 cagcaagcat ttccacctct atggcagcaa cgcctccctg gcccaggttg tcatgaaggt
     8581 tgacgtggtg tatgagaagc agatgctcta cctctacgtg ctgagcggca tcggggggct
     8641 gctgctgctg ctgctcattt tcatagtgct gtacaaggtt ggtttcttca aacggaacct
     8701 gaaggagaag atggaggctg gcagaggtgt cccgaatgga atccctgcag aagactctga
     8761 gcagctggca tctgggcaag aggctgggga tcccggctgc ctgaagcccc tccatgagaa
     8821 ggactctgag agtggtggtg gcaaggactg agcggccgcc actgtgctgg atatctgcag
     8881 aattccacca cactggacta gtggatccga gctcggtacc aagcttaagt ttaaaccgct
     8941 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9001 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9061 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9121 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9181 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     9241 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9301 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9361 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9421 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9481 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9541 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9601 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     9661 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9721 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
//
