LOCUS       sequence-199436-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aL-EGFP with 4aa linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Nordenfelt P, Moore TI, Mehta SB, Kalappurakkal JM, Swaminathan V, 
            Koga N, Lambert TJ, Baker D, Waters JC, Oldenbourg R, Tani T, Mayor 
            S, Waterman CM, Springer TA
  TITLE     Direction of actin flow dictates integrin LFA-1 orientation during 
            leukocyte migration.
  JOURNAL   Nat Commun. 2017 Dec 11;8(1):2047. doi: 10.1038/s41467-017-01848-y.
  PUBMED    29229906
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-12-11"; volume: "8(1):2047. doi"; pages: " 
            10.1038/s41467-017-01848-y"
COMMENT     Sequence Label: aLL-EGFP
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(116..133)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    122..346
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      392..820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(479..498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     689..710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(829..849)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        834..1163
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1014..1149
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1076..1095
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1212..2237
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1212..2237
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    2367..2488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2404..2423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2458..2477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2537..2553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2537..2553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2550..2572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2561..2577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2585..2591,2592..2609,2610..2615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2630..2651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2768..2785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2939..3527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3019..3038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3698..4489,4490..4558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4321..4340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4559..4663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4738..4757)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4929..5308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5309..5512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5557..5576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5557..5575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5793..5804
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(6956..6958,6959..6961,6962..7672)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7001..7022)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7262..7281)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7609..7630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtggtggtgg caaggactga gcggccgcca ctgtgctgga tatctgcaga attccaccac
       61 actggactag tggatccgag ctcggtacca agcttaagtt taaaccgctg atcagcctcg
      121 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      181 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      241 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      301 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
      361 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
      421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      601 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      721 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
      841 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      901 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      961 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     1021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1081 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     1141 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     1201 atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     1261 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     1321 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     1381 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     1441 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     1501 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     1561 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     1621 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     1681 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     1741 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     1801 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     1861 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     1921 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     1981 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     2041 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     2101 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     2161 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     2221 cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     2281 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     2341 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     2401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2461 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     2521 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3361 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3601 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3661 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3721 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3781 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3841 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3901 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3961 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4021 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4081 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4141 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4201 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4261 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4321 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4381 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4441 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4501 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4561 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4621 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4681 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     4741 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     4801 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     4861 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     4921 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     4981 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     5041 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     5101 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     5161 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     5221 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5281 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     5341 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     5401 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     5461 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
     5521 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     5581 ccaagctggc tagcgtttaa acgggccctc tagagccacc atgaaggatt cctgcatcac
     5641 tgtgatggcc atggcgctgc tgtctgggtt ctttttcttc gcgccggcct cgagctacaa
     5701 cctggacgtg cggggcgcgc ggagcttctc cccaccgcgc gccgggaggc actttggata
     5761 ccgcgtcctg caggtcggaa acggggtcat cgtgggagct ccaggggagg ggaacagcac
     5821 aggaagcctc tatcagtgcc agtcgggcac aggacactgc ctgccagtca ccctgagagg
     5881 ttccaactat acctccaagt acttgggaat gaccttggca acagacccca cagatggaag
     5941 cattttggcc tgtgaccctg ggctgtctcg aacgtgtgac cagaacacct atctgagtgg
     6001 cctgtgttac ctcttccgcc agaatctgca gggtcccatg ctgcaggggc gccctggttt
     6061 tcaggaatgt atcaagggca acgtagacct ggtatttctg tttgatggtt cgatgagctt
     6121 gcagccagat gaatttcaga aaattctgga cttcatgaag gatgtgatga agaaactcag
     6181 caacacttcg taccagtttg ctgctgttca gttttccaca agctacaaaa cagaatttga
     6241 tttctcagat tatgttaaat ggaaggaccc tgatgctctg ctgaagcatg taaagcacat
     6301 gttgctgttg accaatacct ttggtgccat caattatgtc gcgacagagg tgttccggga
     6361 ggagctgggg gcccggccag atgccaccaa agtgcttatc atcatcacgg atggggaggc
     6421 cactgacagt ggcaacatcg atgcggccaa agacatcatc cgctacatca tcgggattgg
     6481 aaagcatttt cagaccaagg agagtcagga gaccctccac aaatttgcat caaaacccgc
     6541 gagcgagttt gtgaaaattc tggacacatt tgagaagctg aaagatctat tcactgagct
     6601 gcagaagaag atctatgtca ttgagggcac aagcaaacag gacctgactt ccttcaacat
     6661 ggagctgtcc tccagcggca tcagtgctga cctcagcagg ggccatgcag tcgtgggggc
     6721 agtaggagcc aaggactggg ctgggggctt tcttgacctg aaggcagacc tgcaggatga
     6781 cacatttatt gggaatgaac cattgacacc agaagtgaga gcaggctatt tgggttacac
     6841 cgtgacctgg ctgccctccc ggcaaaagac ttcgttgctg gcctcgggag cccctcgata
     6901 ccagcacatg ggccgagtgc tgctgttcca agagccacag ggctctggat ctggcatggt
     6961 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7021 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7081 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7141 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     7201 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7261 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7321 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7381 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     7441 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     7501 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     7561 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     7621 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggctcagg
     7681 cagcggagga cactggagcc aggtccagac aatccatggg acccagattg gctcttattt
     7741 cggtggggag ctgtgtggcg tcgacgtgga ccaagatggg gagacagagc tgctgctgat
     7801 tggtgcccca ctgttctatg gggagcagag aggaggccgg gtgtttatct accagagaag
     7861 acagttgggg tttgaagaag tctcagagct gcagggggac cccggctacc cactcgggcg
     7921 gtttggagaa gccatcactg ctctgacaga catcaacggc gatgggctgg tagacgtggc
     7981 tgtgggggcc cctctggagg agcagggggc tgtgtacatc ttcaatggga ggcacggggg
     8041 gcttagtccc cagccaagtc agcggataga agggacccaa gtgctctcag gaattcagtg
     8101 gtttggacgc tccatccatg gggtgaagga ccttgaaggg gatggcttgg cagatgtggc
     8161 tgtgggggct gagagccaga tgatcgtgct gagctcccgg cccgtggtgg atatggtcac
     8221 cctgatgtcc ttctctccag ctgagatccc agtgcatgaa gtggagtgct cctattcaac
     8281 cagtaacaag atgaaagaag gagttaatat cacaatctgt ttccagatca agtctctcat
     8341 cccccagttc caaggccgcc tggttgccaa tctcacttac actctgcagc tggatggcca
     8401 ccggaccaga agacgggggt tgttcccagg agggagacat gaactcagaa ggaatatagc
     8461 tgtcaccacc agcatgtcat gcactgactt ctcatttcat ttcccggtat gtgttcaaga
     8521 cctcatctcc cccatcaatg tttccctgaa tttctctctt tgggaggagg aagggacacc
     8581 gagggaccaa agggcgcagg gcaaggacat accgcccatc ctgagaccct ccctgcactc
     8641 ggaaacctgg gagatccctt ttgagaagaa ctgtggggag gacaagaagt gtgaggcaaa
     8701 cttgagagtg tccttctctc ctgcaagatc cagagccctg cgtctaactg cttttgccag
     8761 cctctctgtg gagctgagcc tgagtaactt ggaagaagat gcttactggg tccagctgga
     8821 cctgcacttc cccccgggac tctccttccg caaggtggag atgctgaagc cccatagcca
     8881 gatacctgtg agctgcgagg agcttcctga agagtccagg cttctgtcca gggcattatc
     8941 ttgcaatgtg agctctccca tcttcaaagc aggccactcg gttgctctgc agatgatgtt
     9001 taatacactg gtaaacagct cctgggggga ctcggttgaa ttgcacgcca atgtgacctg
     9061 taacaatgag gactcagacc tcctggagga caactcagcc actaccatca tccccatcct
     9121 gtaccccatc aacatcctca tccaggacca agaagactcc acactctatg tcagtttcac
     9181 ccccaaaggc cccaagatcc accaagtcaa gcacatgtac caggtgagga tccagccttc
     9241 catccacgac cacaacatac ccaccctgga ggctgtggtt ggggtgccac agcctcccag
     9301 cgaggggccc atcacacacc agtggagcgt gcagatggag cctcccgtgc cctgccacta
     9361 tgaggatctg gagaggctcc cggatgcagc tgagccttgt ctccccggag ccctgttccg
     9421 ctgccctgtt gtcttcaggc aggagatcct cgtccaagtg atcgggactc tggagctggt
     9481 gggagagatc gaggcctctt ccatgttcag cctctgcagc tccctctcca tctccttcaa
     9541 cagcagcaag catttccacc tctatggcag caacgcctcc ctggcccagg ttgtcatgaa
     9601 ggttgacgtg gtgtatgaga agcagatgct ctacctctac gtgctgagcg gcatcggggg
     9661 gctgctgctg ctgctgctca ttttcatagt gctgtacaag gttggtttct tcaaacggaa
     9721 cctgaaggag aagatggagg ctggcagagg tgtcccgaat ggaatccctg cagaagactc
     9781 tgagcagctg gcatctgggc aagaggctgg ggatcccggc tgcctgaagc ccctccatga
     9841 gaaggactct gaga
//
