LOCUS       sequence-199437-        5892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pombe General Purpose Vector - with Sp.his3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     Construction of Designer Selectable Marker Deletions with 
            CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized 
            Design of Common Entry Vectors.
  JOURNAL   G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363.
  PUBMED    29321167
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363."
COMMENT     Sequence Label: pYZ192
FEATURES             Location/Qualifiers
     source          1..5892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             217..597
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    273..380
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     297..313
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     297..313
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(347..363)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(348..364)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(388..407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(389..407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(417..434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(417..433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(426..448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      574..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(661..680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     871..892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2828..3611
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        3987..4091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4092..4160,4161..4952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4310..4329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5123..5711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5612..5631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5865..5882
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
      301 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
      361 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      541 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      601 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      781 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      901 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      961 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     1021 ttacaattta aattagcgta actcctttac aaaattaaaa ataacatctt ttcattgctt
     1081 atcgaactat acggaaacac accaaaaagg aagaaaacgt attttaataa agaaagaagg
     1141 aatgcaagcg caattagcga acacaaaata tattgggtac atcagaaaag aaatgtgacg
     1201 agtccggaag gggaaaggaa agagttataa tttgtctaat tgaaacgttc taataggcca
     1261 gaaaaggaaa cctcattttt ttgtatagta ttcctgcaat acaagtttaa tagtttttag
     1321 taatatcgta ttctcttctt ctgtgccaac agtgatacgc aatgctcctt cgcaaagagg
     1381 ctctgtacct ctaaatctaa caacgacttt gtgcatcgtg gccatttgta aataaagata
     1441 ttttgcggca tcgttactag gcttacctcc ttctggtcta tctaaaacct gaatgaggat
     1501 aaaatttgca tcataaccac ctataatctt gcccattcct ttaatggtag taagctcttt
     1561 gcaaagacga actctttgct gtataatagc atctctatat gtatgcattt tatcaatcga
     1621 ctgaggggat aaagcatcca aagctaaacg agaggtgggt tcgctaatgt tataaggagc
     1681 ctttaatgag ttcataatgg tagcaatagg tttacttgtc aaacaaaatc cgatacgaat
     1741 acctgctaga ccgaaagatt tcgaaagggt ttggcaaaca gcaaggtttg gatattcatt
     1801 gacaagagtt aaggcagaca tatcgggagc agagaaatca atataggcct catcgacgac
     1861 aacgattcca ttccatgtag ggtgctccaa aatcttctta atatcctcca gtttaagagc
     1921 cttagctgtt ggattaccag gggaacaagc aaaaaagact ttgatggcac tatctttaga
     1981 aagtgtttcg caaatagcat cgacgttcaa attgaaatct ggttcaagga gaaccttaac
     2041 aacctcaaca tcgttgattt ttgcagacac cgtatacatt ccgtacgaag gtggacacat
     2101 caaaatctta tcttttccag gaatacaaga aatgcgaatt aaggagtcaa taatttcatc
     2161 gctgccaaca cccatgcaaa tgttatctgg tgttagtggc ttggtaatgg aaagctcttt
     2221 gtttctcaaa tcacataatc tttgctttac ttcgatctgt ctaggatcgg gataacgatt
     2281 aaattccaca ccgtccacgg aaataaccga accataagca cattcattgg catcaagaag
     2341 gacaccttca gagaagtcat ctctcgcaca gcgataaggc tgaagttcta agatattttt
     2401 acgaagacaa gtattcaaat caaacatcgt atctctttcg atgtgttttt gttaaagtaa
     2461 gaagaaagcg atcgatgaga ttaattaata attaacctaa gatgcaggtg atatctataa
     2521 atatatagtg tgtagcaatg tgagtcttta attcattaca acgtgtatca ccgtgtcatt
     2581 atatccaata aattcagaaa cctgaagagg aaagagaaat aaagctcgat atgtctttca
     2641 tttccaaata caattataag tgcaatagta aagaaaacgt tgaaagcttg ataaaaattt
     2701 cgttactata ctaatgctgt aaagaaaatg caccatgtcg atcgactcta gaggaaattc
     2761 gagtctaact ccttaaccac tcggacatag tgacttatct gacactcatt gtaaattaat
     2821 atatatatag gcattttgtt tagttaaagg tacttaagta attagtataa acgaaccaat
     2881 tttataatca ggaagttaag tgaatggtag cacatgtcgt aaaaattgtg aatttttatt
     2941 gaataatatt ttaaatacaa gcctttctag actaggtata ctcataaaca tatatgagca
     3001 aaaggataga ggagattaca ttgcatcttc tacaaattta tttattgccc tttactgaaa
     3061 aattaaataa tgagtactaa atgataaaaa gcgctcagta caagaaagat tgcaaaaata
     3121 ttgcattctt catgaattaa agttgcatat aaggcatatt gaaagtaata gtactaaaac
     3181 agcagttagc gaaaattaat agaattatat tcgcaagaca attgtgacat taaattaaaa
     3241 aattgtagaa tttttactat cctctttaac gccatgagcc tttataaaaa ggttaaatta
     3301 gttttaacat tctttttttg agtaagagtt tacaatttat caaaacctgt gttattatat
     3361 tcattagttt caatttatta gcatctagag aaaaatcaat tggcagttac attgttggaa
     3421 tttatgaaga aaaagaatct acaacggaga ataagttgct gatcgctttc cctaaaattg
     3481 tatattttgc tgagcttatt ttgacatttc gttgaagttt tctctaattc gcattcattt
     3541 taagtaaaac aatgagaaat aaaattacaa aaaatacaaa ttaaaataca atttttagct
     3601 ataattatag acgatgccct tgtatcccat tctgtctcgc ttgcccctac tttttatctt
     3661 ttatatacca taatgaacgc tgccgctact aaccataccc cgattttaca tttcggactc
     3721 ccaaggacgt acaaaataga aaactataga aaaaaataat cagaaaatag catgtcatct
     3781 ctttgtaaaa cgcgtttgca agaagaaagg aaacaatgga gaagagatca tccatttgta
     3841 tgtaaatttt agtaaacttg aagaaatcac taacaacttc tcttacttag ggattctatg
     3901 caaaaccttg taaatcatct gatggaggac tcgatttaat gaattggaag gttggatttc
     3961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4801 agcgtggatc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5881 aggaagcgga ag
//
