LOCUS       sequence-199438-        9726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length human aL with moxsynGFP in betapropeller.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Nordenfelt P, Moore TI, Mehta SB, Kalappurakkal JM, Swaminathan V, 
            Koga N, Lambert TJ, Baker D, Waters JC, Oldenbourg R, Tani T, Mayor 
            S, Waterman CM, Springer TA
  TITLE     Direction of actin flow dictates integrin LFA-1 orientation during 
            leukocyte migration.
  JOURNAL   Nat Commun. 2017 Dec 11;8(1):2047. doi: 10.1038/s41467-017-01848-y.
  PUBMED    29229906
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-12-11"; volume: "8(1):2047. doi"; pages: " 
            10.1038/s41467-017-01848-y"
COMMENT     Sequence Label: aL-L3
FEATURES             Location/Qualifiers
     source          1..9726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      41..176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     103..122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             239..1264
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             239..1264
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1394..1515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1431..1450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1485..1504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1564..1580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1564..1580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1577..1599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1588..1604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1612..1618,1619..1636,1637..1642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1795..1812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1966..2554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2046..2065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2725..3516,3517..3585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3348..3367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3586..3690)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3765..3784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3956..4335
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4336..4539
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4584..4603
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4584..4602
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4800..4811
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(8869..8886)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8875..9099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9145..9573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9232..9251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9442..9463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9582..9602)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9587..9726,1..190)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
       61 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      121 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      181 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat
      241 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      301 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      361 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
      421 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
      481 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
      541 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
      601 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
      661 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
      721 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
      781 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
      841 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
      901 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
      961 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     1021 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     1081 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     1141 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     1201 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     1261 atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     1321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     1561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3721 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3781 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3841 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3901 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3961 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4021 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4081 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4141 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4201 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4261 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4321 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4381 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4441 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4501 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     4561 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     4621 cgccaccatg aaggattcct gcatcactgt gatggccatg gcgctgctgt ctgggttctt
     4681 tttcttcgcg ccggcctcga gctacaacct ggacgtgcgg ggcgcgcgga gcttctcccc
     4741 accgcgcgcc gggaggcact ttggataccg cgtcctgcag gtcggaaacg gggtcatcgt
     4801 gggagctcca ggggagggga acagcacagg aagcctctat cagtgccagt cgggcacagg
     4861 acactgcctg ccagtcaccc tgagaggttc caactatacc tccaagtact tgggaatgac
     4921 cttggcaaca gaccccacag atggaagcat tttggcctgt gaccctgggc tgtctcgaac
     4981 gtgtgaccag aacacctatc tgagtggcct gtgttacctc ttccgccaga atctgcaggg
     5041 tcccatgctg caggggcgcc ctggttttca ggaatgtatc aagggcaacg tagacctggt
     5101 atttctgttt gatggttcga tgagcttgca gccagatgaa tttcagaaaa ttctggactt
     5161 catgaaggat gtgatgaaga aactcagcaa cacttcgtac cagtttgctg ctgttcagtt
     5221 ttccacaagc tacaaaacag aatttgattt ctcagattat gttaaatgga aggaccctga
     5281 tgctctgctg aagcatgtaa agcacatgtt gctgttgacc aatacctttg gtgccatcaa
     5341 ttatgtcgcg acagaggtgt tccgggagga gctgggggcc cggccagatg ccaccaaagt
     5401 gcttatcatc atcacggatg gggaggccac tgacagtggc aacatcgatg cggccaaaga
     5461 catcatccgc tacatcatcg ggattggaaa gcattttcag accaaggaga gtcaggagac
     5521 cctccacaaa tttgcatcaa aacccgcgag cgagtttgtg aaaattctgg acacatttga
     5581 gaagctgaaa gatctattca ctgagctgca gaagaagatc tatgtcattg agggcacaag
     5641 caaacaggac ctgacttcct tcaacatgga gctgtcctcc agcggcatca gtgctgacct
     5701 cagcaggggc catgcagtcg tgggggcagt aggagccaag gactgggctg ggggctttct
     5761 tgacctgaag gcagacctgc aggatgacac atttattggg aatgaaccat tgacaccaga
     5821 agtgagagca ggctatttgg gttacaccgt gacctggctg ccctcccggc aaaagacttc
     5881 gttgctggcc tcgggagccc ctcgatacca gcacatgggc cgagtgctgc tgttccaaga
     5941 gccacaggct gaggaactgt ttactggagt ggtccctatt ctggtggagc tggatggaga
     6001 tgtcaacggg cacaagttta gcgtgcgagg agagggagaa ggagacgcta ccaatgggaa
     6061 gctgacactg aaattcatca gcaccacagg aaagctgccc gtgccttggc caactctggt
     6121 cactaccctg acctacggcg tgcagagctt ttcccggtat ccagaccaca tgaagagaca
     6181 tgatttcttt aaaagcgcaa tgcccgaggg atacgtccag gaaaggacca tttccttcaa
     6241 ggacgatggc acatataaaa ctcgcgccga ggtgaagttt gaaggcgaca cactggtcaa
     6301 ccgaatcgag ctgaagggga ttgacttcaa agaagatgga aatatcctgg gccacaaact
     6361 ggagtacaac ttcaactctc ataacgtcta catcaccgcc gataagcaga aaaacgggat
     6421 caaggctaac ttcaagatca gacacaacgt ggaagacggc agcgtccagc tggccgatca
     6481 ttaccagcag aacacaccta tcggcgacgg gcccgtgctg ctgcctgata atcactatct
     6541 gtctactcag agtaagctgt caaaagaccc caacgagaag cgggatcata tggtcctgct
     6601 ggagttcgtc accgctgctg gaatcaccca cggaatggat gagctgtatg ctcaacactg
     6661 gagccaggtc cagacaatcc atgggaccca gattggctct tatttcggtg gggagctgtg
     6721 tggcgtcgac gtggaccaag atggggagac agagctgctg ctgattggtg ccccactgtt
     6781 ctatggggag cagagaggag gccgggtgtt tatctaccag agaagacagt tggggtttga
     6841 agaagtctca gagctgcagg gggaccccgg ctacccactc gggcggtttg gagaagccat
     6901 cactgctctg acagacatca acggcgatgg gctggtagac gtggctgtgg gggcccctct
     6961 ggaggagcag ggggctgtgt acatcttcaa tgggaggcac ggggggctta gtccccagcc
     7021 aagtcagcgg atagaaggga cccaagtgct ctcaggaatt cagtggtttg gacgctccat
     7081 ccatggggtg aaggaccttg aaggggatgg cttggcagat gtggctgtgg gggctgagag
     7141 ccagatgatc gtgctgagct cccggcccgt ggtggatatg gtcaccctga tgtccttctc
     7201 tccagctgag atcccagtgc atgaagtgga gtgctcctat tcaaccagta acaagatgaa
     7261 agaaggagtt aatatcacaa tctgtttcca gatcaagtct ctcatccccc agttccaagg
     7321 ccgcctggtt gccaatctca cttacactct gcagctggat ggccaccgga ccagaagacg
     7381 ggggttgttc ccaggaggga gacatgaact cagaaggaat atagctgtca ccaccagcat
     7441 gtcatgcact gacttctcat ttcatttccc ggtatgtgtt caagacctca tctcccccat
     7501 caatgtttcc ctgaatttct ctctttggga ggaggaaggg acaccgaggg accaaagggc
     7561 gcagggcaag gacataccgc ccatcctgag accctccctg cactcggaaa cctgggagat
     7621 cccttttgag aagaactgtg gggaggacaa gaagtgtgag gcaaacttga gagtgtcctt
     7681 ctctcctgca agatccagag ccctgcgtct aactgctttt gccagcctct ctgtggagct
     7741 gagcctgagt aacttggaag aagatgctta ctgggtccag ctggacctgc acttcccccc
     7801 gggactctcc ttccgcaagg tggagatgct gaagccccat agccagatac ctgtgagctg
     7861 cgaggagctt cctgaagagt ccaggcttct gtccagggca ttatcttgca atgtgagctc
     7921 tcccatcttc aaagcaggcc actcggttgc tctgcagatg atgtttaata cactggtaaa
     7981 cagctcctgg ggggactcgg ttgaattgca cgccaatgtg acctgtaaca atgaggactc
     8041 agacctcctg gaggacaact cagccactac catcatcccc atcctgtacc ccatcaacat
     8101 cctcatccag gaccaagaag actccacact ctatgtcagt ttcaccccca aaggccccaa
     8161 gatccaccaa gtcaagcaca tgtaccaggt gaggatccag ccttccatcc acgaccacaa
     8221 catacccacc ctggaggctg tggttggggt gccacagcct cccagcgagg ggcccatcac
     8281 acaccagtgg agcgtgcaga tggagcctcc cgtgccctgc cactatgagg atctggagag
     8341 gctcccggat gcagctgagc cttgtctccc cggagccctg ttccgctgcc ctgttgtctt
     8401 caggcaggag atcctcgtcc aagtgatcgg gactctggag ctggtgggag agatcgaggc
     8461 ctcttccatg ttcagcctct gcagctccct ctccatctcc ttcaacagca gcaagcattt
     8521 ccacctctat ggcagcaacg cctccctggc ccaggttgtc atgaaggttg acgtggtgta
     8581 tgagaagcag atgctctacc tctacgtgct gagcggcatc ggggggctgc tgctgctgct
     8641 gctcattttc atagtgctgt acaaggttgg tttcttcaaa cggaacctga aggagaagat
     8701 ggaggctggc agaggtgtcc cgaatggaat ccctgcagaa gactctgagc agctggcatc
     8761 tgggcaagag gctggggatc ccggctgcct gaagcccctc catgagaagg actctgagag
     8821 tggtggtggc aaggactgat aaaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc
     8881 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     8941 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9001 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9061 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9121 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9181 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9241 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9301 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9361 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9421 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9481 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9541 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     9601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9721 gcatct
//
