LOCUS       sequence-199449-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mouse mCherry (with STOP and extra-sequence) with homology arms for 
            Adcy3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, 
            Paidemarry S, Green R, Reed RR, Seydoux G
  TITLE     Precision genome editing using synthesis-dependent repair of 
            Cas9-induced DNA breaks.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114.
  PUBMED    29183983
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114."
COMMENT     Sequence Label: pBS-AC3CtermGenomic-mCherry
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             509..1216
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(651..669)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     919..938
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgtgggcatt aggtcagtgt tggcagtgcc acagggccaa ggaaacatgg actgaggcct
       61 ctaaggcttt gattcactca aaacatttaa gattcaggtt tgggtggaaa cggcctccgt
      121 tcggtagagt taacaactaa actgtcttga acctgtatca gtgccatgaa atggtagtct
      181 gctgcttaag tgactgcctg tgggtctccc ttatgtgggg ctgtatctac atactgctac
      241 tgagcttcta ccccaccatc ttggcttcct cttctctaac tttgtctctg ttctccatgt
      301 acaggtggta gaagagacgc aggtcatcct tcgagagtac ggcttccgct ttgtgaggcg
      361 aggtcccatc tttgtgaaag gcaaagggga gcttctgacc tttttcttga aggggcggga
      421 caggccagct gccttcccca atggctcctc tgttacactg ccccaccaag tggtggacaa
      481 cccggatcca ccggtcgcca ccctcgaggt gagcaagggc gaggaggata acatggccat
      541 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      601 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      661 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      721 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      781 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      841 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      901 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      961 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     1021 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     1081 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     1141 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1201 cgagctgtac aagtagtcta gagacctctg gctccacagt ggtccacata ggaaggggga
     1261 tttttttgga agcagtgaaa agctttggga aaggacaaat gaccaagtcc tggtgttccc
     1321 aattattgaa gcgcacattc catagacttt aggtttcaga tttcctccag ccttcctgtg
     1381 tggatgtgag ctctgagggt ggctattcct actcctcagt gagcctgtag cttccccaaa
     1441 gcaggagtct taggcatagt actggagcag cctttccaga gcctttgttc cagctcagcc
     1501 cttcacccct agagaggcca tggccactgt gagcaggatg ccagccgagg cggaacgaag
     1561 ctgcctgtgc tgggggcggg gagctccggt tgggctcagg gctgaggagg ctctgcaccc
     1621 ggctgctcct tcctgtctgg tta
//
