LOCUS       sequence-199460-        8690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hGCN5
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             618..2111
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2121..2123,2124..2126,2127..2837)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2166..2187)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2427..2446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2774..2795
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5471..5592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5508..5527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5562..5581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5599..6054)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5736..5757)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5948..5967
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6081..6185
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6187..6544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6187..6378
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6210..6230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6395..6530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6579..7373
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6579..7373
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6633..6652)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7243..7262
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7561..7580)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7605..7652
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7981..8569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8470..8489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      601 cagatctcga ggccaccatg aagatggaca aaaagactat agtttggttt agaagagacc
      661 taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg
      721 tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt
      781 ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca
      841 ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg
      901 ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg
      961 taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt
     1021 atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact
     1081 ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga
     1141 tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg
     1201 agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct ccaggatgga
     1261 gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga
     1321 acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc catttcgggg
     1381 aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag
     1441 ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag
     1501 agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc
     1561 atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca
     1621 ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc
     1681 ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga
     1741 aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc
     1801 ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca
     1861 atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc
     1921 ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg
     1981 tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg
     2041 acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga
     2101 tcggagcagc agcccgggtc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2161 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2221 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2281 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc
     2341 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2401 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2461 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2521 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2581 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2641 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2701 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2761 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2821 tggacgagct gtacaagagc ggccgcatgg cggaaccttc ccaggccccg accccggccc
     2881 cggctgcgca gccccggccc cttcagtccc cagcccctgc cccaactccg actcctgcac
     2941 ccagcccggc ttcagccccg attccgactc ccaccccggc accagcccct gccccagctg
     3001 cagccccagc cggcagcaca gggactgggg ggcccggggt aggaagtggg ggggccggga
     3061 gcggggggga tccggctcga cctggcctga gccagcagca gcgcgccagt cagaggaagg
     3121 cgcaagtccg ggggctgccg cgcgccaaga agcttgagaa gctaggggtc ttctcggctt
     3181 gcaaggccaa tgaaacctgt aagtgtaatg gctggaaaaa ccccaagccc cccactgcac
     3241 cccgcatgga tctgcagcag ccagctgcca acctgagtga gctgtgccgc agttgtgagc
     3301 accccttggc tgaccacgta tcccacttgg agaatgtgtc agaggatgag ataaaccgac
     3361 tgctggggat ggtggtggat gtggagaatc tcttcatgtc tgttcacaag ggagaggaca
     3421 cagacaccaa tcaggtctat ttctacctct tcaagctact gcggaaatgc atcctgcaga
     3481 tgacccggcc tgtggtggag gggtccctgg gcagccctcc atttgagaaa cctaatattg
     3541 agcagggtgt gctgaacttt gtgcagtaca aatttagtca cctggctccc cgggagcggc
     3601 agacgatgtt cgagctctca aagatgttct tgctctgcct taactactgg aagcttgaga
     3661 cacctgccca gtttcggcag aggtctcagg ctgaggacgt ggctacctac aaggtcaatt
     3721 acaccagatg gctctgttac tgccacgtgc cccagagctg tgatagcctc ccccgctacg
     3781 aaaccactca tgtctttggg cgaagccttc tccggtccat tttcaccgtt acccgccggc
     3841 agctgctgga aaagttccga gtggagaagg acaaattggt gcccgagaag aggaccctca
     3901 tcctcactca cttccccaaa ttcctgtcca tgctggagga ggagatctat ggggcaaact
     3961 ctccaatctg ggagtcaggc ttcaccatgc caccctcaga ggggacacag ctggttcccc
     4021 ggccagcttc agtcagtgca gcggttgttc ccagcacccc catcttcagc cccagcatgg
     4081 gtgggggcag caacagctcc ctgagtctgg attctgcagg ggccgagcct atgccaggcg
     4141 agaagaggac gctcccagag aacctgaccc tggaggatgc caagcggctc cgtgtgatgg
     4201 gtgacatccc catggagctg gtcaatgagg tcatgctgac catcactgac cctgctgcca
     4261 tgctggggcc tgagacgagc ctgctttcgg ccaatgcggc ccgggatgag acagcccgcc
     4321 tggaggagcg ccgcggcatc atcgagttcc atgtcatcgg caactcactg acgcccaagg
     4381 ccaaccggcg ggtgttgctg tggctcgtgg ggctgcagaa tgtcttttcc caccagctgc
     4441 cgcgcatgcc taaggagtat atcgcccgcc tcgtctttga cccgaagcac aagactctgg
     4501 ccttgatcaa ggatgggcgg gtcatcggtg gcatctgctt ccgcatgttt cccacccagg
     4561 gcttcacgga gattgtcttc tgtgctgtca cctcgaatga gcaggtcaag ggttatggga
     4621 cccacctgat gaaccacctg aaggagtatc acatcaagca caacattctc tacttcctca
     4681 cctacgccga cgagtacgcc atcggctact tcaaaaagca gggtttctcc aaggacatca
     4741 aggtgcccaa gagccgctac ctgggctaca tcaaggacta cgagggagcg acgctgatgg
     4801 agtgtgagct gaatccccgc atcccctaca cggagctgtc ccacatcatc aagaagcaga
     4861 aagagatcat caagaagctg attgagcgca aacaggccca gatccgcaag gtctacccgg
     4921 ggctcagctg cttcaaggag ggcgtgaggc agatccctgt ggagagcgtt cctggcattc
     4981 gagagacagg ctggaagcca ttggggaagg agaaggggaa ggagctgaag gaccccgacc
     5041 agctctacac aaccctcaaa aacctgctgg cccaaatcaa gtctcacccc agtgcctggc
     5101 ccttcatgga gcctgtgaag aagtcggagg cccctgacta ctacgaggtc atccgcttcc
     5161 ccattgacct gaagaccatg actgagcggc tgcgaagccg ctactacgtg acccggaagc
     5221 tctttgtggc cgacctgcag cgggtcatcg ccaactgtcg cgagtacaac cccccggaca
     5281 gcgagtactg ccgctgtgcc agcgccctgg agaagttctt ctacttcaag ctcaaggagg
     5341 gaggcctcat tgacaagtag tctagatcat aatcagccat accacatttg tagaggtttt
     5401 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5461 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5521 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5581 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     5641 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     5701 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     5761 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     5821 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     5881 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     5941 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6001 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     6061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6181 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6361 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6541 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6601 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6661 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6721 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     6781 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6841 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6901 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6961 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7021 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7081 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     7141 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7201 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7261 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7321 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7381 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7441 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7501 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     7561 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     7621 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     7681 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     7741 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     7801 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     7861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8641 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
//
