LOCUS       sequence-199461-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hGCN5-NLS
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             618..2111
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2121..2123,2124..2126,2127..2837)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2166..2187)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2427..2446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2774..2795
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5358..5378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5492..5613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5529..5548)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5583..5602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5620..6075)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5757..5778)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5969..5988
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6102..6206
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6208..6565
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6208..6399
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6231..6251)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6416..6551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6600..7394
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6600..7394
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6654..6673)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7264..7283
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7582..7601)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7626..7673
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8002..8590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8491..8510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      601 cagatctcga ggccaccatg aagatggaca aaaagactat agtttggttt agaagagacc
      661 taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg
      721 tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt
      781 ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca
      841 ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg
      901 ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg
      961 taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt
     1021 atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact
     1081 ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga
     1141 tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg
     1201 agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct ccaggatgga
     1261 gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga
     1321 acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc catttcgggg
     1381 aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag
     1441 ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag
     1501 agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc
     1561 atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca
     1621 ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc
     1681 ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga
     1741 aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc
     1801 ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca
     1861 atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc
     1921 ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg
     1981 tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg
     2041 acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga
     2101 tcggagcagc agcccgggtc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2161 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2221 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2281 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc
     2341 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2401 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2461 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2521 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2581 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2641 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2701 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2761 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2821 tggacgagct gtacaagagc ggccgcatgg cggaaccttc ccaggccccg accccggccc
     2881 cggctgcgca gccccggccc cttcagtccc cagcccctgc cccaactccg actcctgcac
     2941 ccagcccggc ttcagccccg attccgactc ccaccccggc accagcccct gccccagctg
     3001 cagccccagc cggcagcaca gggactgggg ggcccggggt aggaagtggg ggggccggga
     3061 gcggggggga tccggctcga cctggcctga gccagcagca gcgcgccagt cagaggaagg
     3121 cgcaagtccg ggggctgccg cgcgccaaga agcttgagaa gctaggggtc ttctcggctt
     3181 gcaaggccaa tgaaacctgt aagtgtaatg gctggaaaaa ccccaagccc cccactgcac
     3241 cccgcatgga tctgcagcag ccagctgcca acctgagtga gctgtgccgc agttgtgagc
     3301 accccttggc tgaccacgta tcccacttgg agaatgtgtc agaggatgag ataaaccgac
     3361 tgctggggat ggtggtggat gtggagaatc tcttcatgtc tgttcacaag ggagaggaca
     3421 cagacaccaa tcaggtctat ttctacctct tcaagctact gcggaaatgc atcctgcaga
     3481 tgacccggcc tgtggtggag gggtccctgg gcagccctcc atttgagaaa cctaatattg
     3541 agcagggtgt gctgaacttt gtgcagtaca aatttagtca cctggctccc cgggagcggc
     3601 agacgatgtt cgagctctca aagatgttct tgctctgcct taactactgg aagcttgaga
     3661 cacctgccca gtttcggcag aggtctcagg ctgaggacgt ggctacctac aaggtcaatt
     3721 acaccagatg gctctgttac tgccacgtgc cccagagctg tgatagcctc ccccgctacg
     3781 aaaccactca tgtctttggg cgaagccttc tccggtccat tttcaccgtt acccgccggc
     3841 agctgctgga aaagttccga gtggagaagg acaaattggt gcccgagaag aggaccctca
     3901 tcctcactca cttccccaaa ttcctgtcca tgctggagga ggagatctat ggggcaaact
     3961 ctccaatctg ggagtcaggc ttcaccatgc caccctcaga ggggacacag ctggttcccc
     4021 ggccagcttc agtcagtgca gcggttgttc ccagcacccc catcttcagc cccagcatgg
     4081 gtgggggcag caacagctcc ctgagtctgg attctgcagg ggccgagcct atgccaggcg
     4141 agaagaggac gctcccagag aacctgaccc tggaggatgc caagcggctc cgtgtgatgg
     4201 gtgacatccc catggagctg gtcaatgagg tcatgctgac catcactgac cctgctgcca
     4261 tgctggggcc tgagacgagc ctgctttcgg ccaatgcggc ccgggatgag acagcccgcc
     4321 tggaggagcg ccgcggcatc atcgagttcc atgtcatcgg caactcactg acgcccaagg
     4381 ccaaccggcg ggtgttgctg tggctcgtgg ggctgcagaa tgtcttttcc caccagctgc
     4441 cgcgcatgcc taaggagtat atcgcccgcc tcgtctttga cccgaagcac aagactctgg
     4501 ccttgatcaa ggatgggcgg gtcatcggtg gcatctgctt ccgcatgttt cccacccagg
     4561 gcttcacgga gattgtcttc tgtgctgtca cctcgaatga gcaggtcaag ggttatggga
     4621 cccacctgat gaaccacctg aaggagtatc acatcaagca caacattctc tacttcctca
     4681 cctacgccga cgagtacgcc atcggctact tcaaaaagca gggtttctcc aaggacatca
     4741 aggtgcccaa gagccgctac ctgggctaca tcaaggacta cgagggagcg acgctgatgg
     4801 agtgtgagct gaatccccgc atcccctaca cggagctgtc ccacatcatc aagaagcaga
     4861 aagagatcat caagaagctg attgagcgca aacaggccca gatccgcaag gtctacccgg
     4921 ggctcagctg cttcaaggag ggcgtgaggc agatccctgt ggagagcgtt cctggcattc
     4981 gagagacagg ctggaagcca ttggggaagg agaaggggaa ggagctgaag gaccccgacc
     5041 agctctacac aaccctcaaa aacctgctgg cccaaatcaa gtctcacccc agtgcctggc
     5101 ccttcatgga gcctgtgaag aagtcggagg cccctgacta ctacgaggtc atccgcttcc
     5161 ccattgacct gaagaccatg actgagcggc tgcgaagccg ctactacgtg acccggaagc
     5221 tctttgtggc cgacctgcag cgggtcatcg ccaactgtcg cgagtacaac cccccggaca
     5281 gcgagtactg ccgctgtgcc agcgccctgg agaagttctt ctacttcaag ctcaaggagg
     5341 gaggcctcat tgacaagcca aaaaagaaga gaaaggtata gtctagatca taatcagcca
     5401 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5461 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5581 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     5641 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5701 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5761 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5821 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     5881 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5941 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6001 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6061 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6181 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6241 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6301 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6361 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6421 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6481 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6541 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     6601 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6661 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6721 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6781 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6841 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6901 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6961 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7021 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7081 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7141 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7201 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7261 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7321 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7381 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7441 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7501 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7561 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     7621 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     7681 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     7741 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     7801 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     7861 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7921 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7981 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8041 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8101 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8161 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8221 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8281 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8341 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8401 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8461 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8521 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8581 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8641 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     8701 gcattagtta t
//
