LOCUS       sequence-199462-        8690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hGCN5mut
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             622..2115
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2125..2127,2128..2130,2131..2841)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2170..2191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2431..2450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2778..2799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5475..5596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5512..5531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5566..5585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5603..6058)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5740..5761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5952..5971
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6085..6189
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6191..6548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6191..6382
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6214..6234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6399..6534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6583..7377
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6583..7377
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6637..6656)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7247..7266
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7565..7584)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7609..7656
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7985..8573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8474..8493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgaggccac catgaagatg gacaaaaaga ctatagtttg gtttagaaga
      661 gacctaagga ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt
      721 cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga
      781 tggtggatga aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac
      841 ctcactttaa tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc
      901 ggtgctacaa aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat
      961 accgtaaagg agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta
     1021 ttgtatgaac cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct
     1081 tactggaaga aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg
     1141 ttgatgccaa taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg
     1201 ctggagaatg aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga
     1261 tggagcaatg ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca
     1321 aagaacagca agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc
     1381 ggggaaataa gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca
     1441 agagataaga acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta
     1501 agagagtatt ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg
     1561 agtcatcttc ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa
     1621 ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg
     1681 atgcataaca gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca
     1741 tggaaatggg gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac
     1801 atccttggct ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg
     1861 gacaatcccg cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg
     1921 cttcccgagc ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta
     1981 accgtactca aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac
     2041 atcgacacag ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc
     2101 atgatcggag cagcagcccg ggtcatggtg agcaagggcg aggagctgtt caccggggtg
     2161 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2221 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2281 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     2341 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2401 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2461 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2521 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2581 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2641 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2701 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     2761 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2821 ggcatggacg agctgtacaa gagcggccgc atggcggaac cttcccaggc cccgaccccg
     2881 gccccggctg cgcagccccg gccccttcag tccccagccc ctgccccaac tccgactcct
     2941 gcacccagcc cggcttcagc cccgattccg actcccaccc cggcaccagc ccctgcccca
     3001 gctgcagccc cagccggcag cacagggact ggggggcccg gggtaggaag tgggggggcc
     3061 gggagcgggg gggatccggc tcgacctggc ctgagccagc agcagcgcgc cagtcagagg
     3121 aaggcgcaag tccgggggct gccgcgcgcc aagaagcttg agaagctagg ggtcttctcg
     3181 gcttgcaagg ccaatgaaac ctgtaagtgt aatggctgga aaaaccccaa gccccccact
     3241 gcaccccgca tggatctgca gcagccagct gccaacctga gtgagctgtg ccgcagttgt
     3301 gagcacccct tggctgacca cgtatcccac ttggagaatg tgtcagagga tgagataaac
     3361 cgactgctgg ggatggtggt ggatgtggag aatctcttca tgtctgttca caagggagag
     3421 gacacagaca ccaatcaggt ctatttctac ctcttcaagc tactgcggaa atgcatcctg
     3481 cagatgaccc ggcctgtggt ggaggggtcc ctgggcagcc ctccatttga gaaacctaat
     3541 attgagcagg gtgtgctgaa ctttgtgcag tacaaattta gtcacctggc tccccgggag
     3601 cggcagacga tgttcgagct ctcaaagatg ttcttgctct gccttaacta ctggaagctt
     3661 gagacacctg cccagtttcg gcagaggtct caggctgagg acgtggctac ctacaaggtc
     3721 aattacacca gatggctctg ttactgccac gtgccccaga gctgtgatag cctcccccgc
     3781 tacgaaacca ctcatgtctt tgggcgaagc cttctccggt ccattttcac cgttacccgc
     3841 cggcagctgc tggaaaagtt ccgagtggag aaggacaaat tggtgcccga gaagaggacc
     3901 ctcatcctca ctcacttccc caaattcctg tccatgctgg aggaggagat ctatggggca
     3961 aactctccaa tctgggagtc aggcttcacc atgccaccct cagaggggac acagctggtt
     4021 ccccggccag cttcagtcag tgcagcggtt gttcccagca cccccatctt cagccccagc
     4081 atgggtgggg gcagcaacag ctccctgagt ctggattctg caggggccga gcctatgcca
     4141 ggcgagaaga ggacgctccc agagaacctg accctggagg atgccaagcg gctccgtgtg
     4201 atgggtgaca tccccatgga gctggtcaat gaggtcatgc tgaccatcac tgaccctgct
     4261 gccatgctgg ggcctgagac gagcctgctt tcggccaatg cggcccggga tgagacagcc
     4321 cgcctggagg agcgccgcgg catcatcgag ttccatgtca tcggcaactc actgacgccc
     4381 aaggccaacc ggcgggtgtt gctgtggctc gtggggctgc agaatgtctt ttcccaccag
     4441 ctgccgcgca tgcctaagga gtatatcgcc cgcctcgtct ttgacccgaa gcacaagact
     4501 ctggccttga tcaaggatgg gcgggtcatc ggtggcatct gcttccgcat gtttcccacc
     4561 cagggcttca cggagattgt cttctgtgct gtcacctcga atgagcaggt caagggttat
     4621 gggacccacc tgatgaacca cctgaaggag tatcacatca agcacaacat tctctacttc
     4681 ctcacctacg ccgacgagta cgccatcggc gcagcaaaaa agcagggttt ctccaaggac
     4741 atcaaggtgc ccaagagccg ctacctgggc tacatcaagg actacgaggg agcgacgctg
     4801 atggagtgtg agctgaatcc ccgcatcccc tacacggagc tgtcccacat catcaagaag
     4861 cagaaagaga tcatcaagaa gctgattgag cgcaaacagg cccagatccg caaggtctac
     4921 ccggggctca gctgcttcaa ggagggcgtg aggcagatcc ctgtggagag cgttcctggc
     4981 attcgagaga caggctggaa gccattgggg aaggagaagg ggaaggagct gaaggacccc
     5041 gaccagctct acacaaccct caaaaacctg ctggcccaaa tcaagtctca ccccagtgcc
     5101 tggcccttca tggagcctgt gaagaagtcg gaggcccctg actactacga ggtcatccgc
     5161 ttccccattg acctgaagac catgactgag cggctgcgaa gccgctacta cgtgacccgg
     5221 aagctctttg tggccgacct gcagcgggtc atcgccaact gtcgcgagta caaccccccg
     5281 gacagcgagt actgccgctg tgccagcgcc ctggagaagt tcttctactt caagctcaag
     5341 gagggaggcc tcattgacaa gtagtctaga tcataatcag ccataccaca tttgtagagg
     5401 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5461 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5581 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     5641 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5701 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5761 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5821 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5881 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5941 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     6001 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     6061 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6181 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     6241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6301 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6361 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6421 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6481 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6541 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6601 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6661 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6721 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     6781 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6841 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6901 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6961 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7021 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7081 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     7141 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7201 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7261 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7321 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7381 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7441 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7501 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     7561 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     7621 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7681 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7741 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7801 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag
//
