LOCUS       sequence-199464-        6749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-mCherry-hHP1-beta
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        364..567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     518..538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             624..2117
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      2136..2145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2142..2849
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2287..2305)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2555..2574
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3536..3657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3573..3592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3627..3646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3664..4119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3801..3822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4013..4032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4146..4250
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4252..4609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4252..4443
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4275..4295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4460..4595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4644..5438
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4644..5438
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4698..4717)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5308..5327
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5626..5645)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5670..5717
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6046..6634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6535..6554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
      601 cggactcaga tctcgaggcc accatgaaga tggacaaaaa gactatagtt tggtttagaa
      661 gagacctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt
      721 ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa
      781 gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg
      841 acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta
      901 ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc
      961 ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc
     1021 tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt
     1081 cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc
     1141 ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag
     1201 ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag
     1261 gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg
     1321 caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt
     1381 tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg
     1441 caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt
     1501 taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt
     1561 tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac
     1621 aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat
     1681 ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc
     1741 catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg
     1801 acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct
     1861 tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat
     1921 ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt
     1981 taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag
     2041 acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga
     2101 tcatgatcgg agcagcagcc cgggatccac cggtcgccac catggtgagc aagggcgagg
     2161 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     2221 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2281 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     2341 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     2401 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2461 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     2521 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2581 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     2641 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     2701 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     2761 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     2821 ccaccggcgg catggacgag ctgtacaagt ccggaatggg gaaaaaacaa aacaagaaga
     2881 aagtggagga ggtgctagaa gaggaggaag aggaatatgt ggtggaaaaa gttctcgacc
     2941 gtcgagtggt aaagggcaaa gtggagtacc tcctaaagtg gaagggattc tcagatgagg
     3001 acaacacatg ggagccagaa gagaacctgg attgccccga cctcattgct gagtttctgc
     3061 agtcacagaa aacagcacat gagacagata aatcagaggg aggcaagcgc aaagctgatt
     3121 ctgattctga agataaggga gaggagagca aaccaaagaa gaagaaagaa gagtcagaaa
     3181 agccacgagg ctttgctcga ggtttggagc cggagcggat tattggagct acagactcca
     3241 gtggagagct catgttcctg atgaaatgga aaaactctga tgaggctgac ctggtccctg
     3301 ccaaggaagc caatgtcaag tgcccacagg ttgtcatatc cttctatgag gaaaggctga
     3361 cgtggcattc ctacccctcg gaggatgatg acaaaaaaga tgacaagaac taaggatcca
     3421 ccggatctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3481 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     3661 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3721 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3781 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3841 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3901 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3961 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4021 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4081 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     4261 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     4621 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4681 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4741 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4801 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     4861 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4921 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4981 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5041 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5101 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5161 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5221 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5281 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5341 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5401 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5461 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5521 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5581 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     5641 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5701 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     5761 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     5821 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     5881 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6721 tgtggataac cgtattaccg ccatgcatt
//
