LOCUS       sequence-199479-        9907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of V5-NES-APEX2-GFP from human PGK promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC,
            Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel 
            MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
  TITLE     Context-Dependent and Disease-Specific Diversity in Protein 
            Interactions within Stress Granules.
  JOURNAL   Cell. 2018 Jan 25;172(3):590-604.e13. doi: 
            10.1016/j.cell.2017.12.032.
  PUBMED    29373831
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.12"; journalName: "Cell"; date: "2018-01-25- 
            25"; volume: "172"; issue: "3"; pages: "590-604.e13"
COMMENT     Sequence Label: hPGK_V5-APEX2-GFP
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     363..385
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     377..394
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     378..394
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        402..902
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     821..839
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             912..953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             954..1700
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1707..1733
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1791..2453
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    2544..3132
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3015..3026)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3206..3439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3511..3632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3719..3752)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4106..4317
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4330..4598
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    5355..5476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5976..6009)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6067..6566
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6102..6125)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6499..6518
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6593..7165
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          7242..7307
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7375..7392)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7381..7605
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7686..7702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7686..7702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7699..7721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7710..7726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7734..7740,7741..7758,7759..7764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7779..7800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7917..7934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8088..8676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8847..9638,9639..9707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9470..9489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9708..9812)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9880..9898
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta cgccagggtt
      361 ttcccagtca cgacgttgta aaacgacggc cagtgttggg gttgcgcctt ttccaaggca
      421 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
      481 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
      541 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
      601 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
      661 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
      721 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
      781 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
      841 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
      901 aggaattcat gggtaagcct atccctaacc ctctcctcgg tctcgattct acgggaaagt
      961 cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag aagaagctca
     1021 gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca ttccactctg
     1081 ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc aagcaccctg
     1141 ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt ttggagccac
     1201 tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct ggcgttgttg
     1261 ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag gacaagcctg
     1321 agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat ttgagagatg
     1381 tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct gggggtcaca
     1441 ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc tctaatcctc
     1501 ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa ggtctccttc
     1561 agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc gttgacaaat
     1621 atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa aagctttccg
     1681 agcttgggtt tgctgatgcc ctgcagctgc ctcccctgga gcgcctgacc ctggacggtg
     1741 gcggtggctc gggcggtggt gggtcgatgg agagcgacga gagcggcctg cccgccatgg
     1801 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg
     1861 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc
     1921 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca
     1981 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca
     2041 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct
     2101 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca
     2161 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca
     2221 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct
     2281 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc
     2341 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg
     2401 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc
     2461 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc
     2521 gctagagctg aatctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2581 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2641 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2701 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2761 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2821 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2881 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2941 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3001 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3061 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3121 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3181 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     3241 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3301 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3361 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3421 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     3481 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3541 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3601 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3661 taactaaaat tcgagctcgg tacctcgcga atgcatcaga tctttccact caaaacatat
     3721 aacttcgtat agcatacatt atacgaagtt atattcaaaa atctagacag cggccgccac
     3781 tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc cccaaagccc
     3841 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     3901 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     3961 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     4021 cagacacctg gggggatacg gggaaaaggc ctccaaggcc ttaattcggc cgcaaggatc
     4081 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4141 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4201 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4261 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4321 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4381 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4441 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4501 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4561 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     4621 atatcgccac catgacggag tataagccga cagttaggct tgctactagg gacgatgtgc
     4681 cccgcgccgt ccgcacactg gcagcagctt ttgctgacta ccctgcaaca cggcacacgg
     4741 tggacccgga caggcatatc gagagggtaa cagagctgca ggagctgttc ttgacgcggg
     4801 taggtctgga tatcggtaag gtttgggtgg cagacgacgg agcagctgtc gcggtgtgga
     4861 ccaccccgga gagcgttgag gcgggagcgg ttttcgccga aatcgggcct aggatggctg
     4921 agttgtctgg gtccagactg gccgctcaac agcaaatgga gggcttgctg gcaccgcatc
     4981 gcccgaaaga gccagcttgg ttcctggcaa ccgtgggcgt ttcacccgat caccaaggca
     5041 agggtctggg aagtgcagtg gtgctgcccg gagtggaagc cgcagaacgc gcgggggtgc
     5101 ccgcatttct ggagacaagt gcccctagga acctgccctt ctatgagaga cttggtttca
     5161 ccgttacagc tgacgtggag gtgcccgagg gtccccgcac gtggtgcatg actcggaaac
     5221 cgggcgcttg aaatcaacct ctggacgaaa ttccgcggtt attacaaaat ttgtgaaaga
     5281 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5341 cctttgtatc agttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5461 tcatcaatgt atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga
     5521 agctatctgg tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg
     5581 ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc
     5641 ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact
     5701 ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc
     5761 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     5821 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     5881 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     5941 ggctttgggg gggggctgtc cctgatatca aacatataac ttcgtatagc atacattata
     6001 cgaagttatt ttatcacgta agtagaacat gaaataacct agatcggatc cccgtcgact
     6061 gcatgcgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6121 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6181 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6241 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6301 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6361 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6421 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     6481 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6541 cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc ctgatgatgt
     6601 tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg ggactaaacc
     6661 tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa
     6721 ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata
     6781 ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta
     6841 tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga
     6901 gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa
     6961 aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc
     7021 tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat
     7081 taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc
     7141 ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga
     7201 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     7261 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     7321 gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga tcagcctcga
     7381 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7441 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7501 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7561 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7621 gaaccagctg gggctcgaga tccactagtt ctagcctcga ggcatgcaag cttggcgtaa
     7681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8521 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9901 tttcgtc
//
