LOCUS       sequence-199490-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control for FRET biosensor analysis - cannot bind downstream 
            effectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Petrie RJ, Gavara N, Chadwick RS, Yamada KM
  TITLE     Nonpolarized signaling reveals two distinct modes of 3D cell 
            migration.
  JOURNAL   J Cell Biol. 2012 Apr 30;197(3):439-55. doi: 10.1083/jcb.201201124.
  PUBMED    22547408
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-04-30- 30"; volume: "197"; 
            issue: "3"; pages: "439-55"
COMMENT     Sequence Label: pECFP-Rac1Y40C
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..626
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNCSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 ggctgtacaa gtccggactc agatctcgag ctcaagcttc gaattccatg caggccatca
       61 agtgtgtggt ggtgggagac ggagctgtag gtaaaacttg cctactgatc agttacacaa
      121 ccaatgcatt tcctggagaa tatatcccta ctgtctttga caattgttct gccaatgtta
      181 tggtagatgg aaaaccggtg aacctgggct tatgggatac agctggacaa gaagattatg
      241 acagattacg ccccctatcc tatccgcaaa cagatgtgtt cttaatttgc ttttcccttg
      301 tgagtcctgc atcatttgaa aatgtccgtg caaagtggta tcctgaggtg cggcaccact
      361 gtcccaacac tcccatcatc ctagtgggaa ctaaacttga tcttagggat gataaagaca
      421 cgatcgagaa actgaaggag aagaagctga ctcccatcac ctatccgcag ggtctagcca
      481 tggctaagga gattggtgcc gtaaaatacc tggagtgctc ggcgctcaca cagcgaggcc
      541 tcaagacagt gtttgacgaa gcgatccgag cagtcctctg cccgcctccc gtgaagaaga
      601 ggaagagaaa atgcctgctg ttgtaaggat ccaccggatc tagat
//
