LOCUS       sequence-199491-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Petrie RJ, Gavara N, Chadwick RS, Yamada KM
  TITLE     Nonpolarized signaling reveals two distinct modes of 3D cell 
            migration.
  JOURNAL   J Cell Biol. 2012 Apr 30;197(3):439-55. doi: 10.1083/jcb.201201124.
  PUBMED    22547408
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-04-30- 30"; volume: "197"; 
            issue: "3"; pages: "439-55"
COMMENT     Sequence Label: pECFP-Cdc42Q61L
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(48..227,228..230,231..623)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
ORIGIN
        1 ggctgtacaa gtccggactc agatctcgag ctcaagcttc gaattccatg cagacaatta
       61 agtgtgttgt tgtgggcgat ggtgccgttg gtaaaacatg tctcctgata tcctacacaa
      121 caaacaaatt tccatctgag tatgtaccga ctgtttttga caactatgca gtcacggtta
      181 tgattggtgg agagccatat acccttggac tttttgatac tgcagggcta gaggattatg
      241 acagattacg accgctgagt tatccacaaa cagatgtatt tctagtctgt ttttcagtgg
      301 tctctccatc ctcatttgaa aatgtgaagg aaaagtgggt gcctgagata actcaccact
      361 gtccaaagac tcctttcttg cttgttggga cccaaattga tctccgagat gacccctcta
      421 ctatcgagaa acttgccaag aacaaacaga agcctatcac tccagagact gctgaaaagc
      481 tggcccgtga cctgaaggcg gtcaagtatg tggagtgttc tgcactcaca cagaaaggcc
      541 taaagaatgt atttgacgaa gcaatattgg ctgccctgga gcctccagaa ccgaagaaga
      601 gccgcaggtg tgtgctgcta tgaggatcca ccggatctag ataactgatc ataatcagcc
      661 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      721 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      781 acaaata
//
