LOCUS       sequence-199492-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control for FRET biosensor analysis - cannot bind downstream 
            effectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Petrie RJ, Gavara N, Chadwick RS, Yamada KM
  TITLE     Nonpolarized signaling reveals two distinct modes of 3D cell 
            migration.
  JOURNAL   J Cell Biol. 2012 Apr 30;197(3):439-55. doi: 10.1083/jcb.201201124.
  PUBMED    22547408
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-04-30- 30"; volume: "197"; 
            issue: "3"; pages: "439-55"
COMMENT     Sequence Label: pECFP-Cdc42-Y40C
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..626
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNCAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
ORIGIN
        1 gcgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattcc atgcagacaa
       61 ttaagtgtgt tgttgtgggc gatggtgccg ttggtaaaac atgtctcctg atatcctaca
      121 caacaaacaa atttccatct gagtatgtac cgactgtttt tgacaactgt gcagtcacgg
      181 ttatgattgg tggagagcca tatacccttg gactttttga tactgcaggg caagaggatt
      241 atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc tgtttttcag
      301 tggtctctcc atcctcattt gaaaatgtga aggaaaagtg ggtgcctgag ataactcacc
      361 actgtccaaa gactcctttc ttgcttgttg ggacccaaat tgatctccga gatgacccct
      421 ctactatcga gaaacttgcc aagaacaaac agaagcctat cactccagag actgctgaaa
      481 agctggcccg tgacctgaag gcggtcaagt atgtggagtg ttctgcactc acacagaaag
      541 gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca gaaccgaaga
      601 agagccgcag gtgtgtgctg ctatgaggat ccaccggatc tagataactg atcataatca
      661 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac c
//
