LOCUS       sequence-199502-        3010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To study effect of Kindlin 2 on Cell/ECM adhesion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Artym VV, Swatkoski S, Matsumoto K, Campbell CB, Petrie RJ, 
            Dimitriadis EK, Li X, Mueller SC, Bugge TH, Gucek M, Yamada KM
  TITLE     Dense fibrillar collagen is a potent inducer of invadopodia via a 
            specific signaling network.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):331-50. doi: 10.1083/jcb.201405099.
  PUBMED    25646088
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-02-2- 2"; volume: "208"; 
            issue: "3"; pages: "331-50"
COMMENT     Sequence Label: pEGFP Kindlin2
FEATURES             Location/Qualifiers
     source          1..3010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2122..2124,2125..2127,2128..2841)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2122..2124,2125..2127,2128..2838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2167..2188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2428..2447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2775..2796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcggccacc
       61 atggctctgg acgggataag gatgccagat ggctgctacg cggacgggac gtgggaactg
      121 agtgtccatg tgacggacct gaaccgcgat gtcaccctga gagtgaccgg cgaggtgcac
      181 attggaggcg tgatgcttaa gctggtggag aaactcgatg taaaaaaaga ttggtctgac
      241 catgctctct ggtgggaaaa gaagagaact tggcttctga agacacattg gaccttagat
      301 aagtatggta ttcaggcaga tgctaagctt cagttcaccc ctcagcacaa actgctccgc
      361 ctgcagcttc ccaacatgaa gtatgtgaag gtgaaagtga atttctctga tagagtcttc
      421 aaagctgttt ctgacatctg taagactttt aatatcagac accccgaaga actttctctc
      481 ttaaagaaac ccagagatcc aacaaagaaa aaaaagaaga agctagatga ccagtctgaa
      541 gatgaggcac ttgaattaga ggggcctctt atcactcctg gatcaggaag tatatattca
      601 agcccaggac tgtatagtaa aacaatgacc cccacttatg atgctcatga tggaagcccc
      661 ttgtcaccaa cttctgcttg gtttggtgac agtgctttgt cagaaggcaa tcctggtata
      721 cttgctgtca gtcaaccaat cacgtcacca gaaatcttgg caaaaatgtt caagcctcaa
      781 gctcttcttg ataaagcaaa aatcaaccaa ggatggcttg attcctcaag atctctcatg
      841 gaacaagatg tgaaggaaaa tgaggccttg ctgctccgat tcaagtatta cagctttttt
      901 gatttgaatc caaagtatga tgcaatcaga atcaatcagc tttatgagca ggccaaatgg
      961 gccattctcc tggaagagat tgaatgcaca gaagaagaaa tgatgatgtt tgcagccctg
     1021 cagtatcata tcaataagct gtcaatcatg acatcagaga atcatttgaa caacagtgac
     1081 aaagaagttg atgaagttga tgctgccctt tcagacctgg agattactct ggaagggggt
     1141 aaaacgtcaa caattttggg tgacattact tccattcctg aacttgctga ctacattaaa
     1201 gttttcaagc caaaaaagct gactctgaaa ggttacaaac aatattggtg caccttcaaa
     1261 gacacatcca tttcttgtta taagagcaaa gaagaatcca gtggcacacc agctcatcag
     1321 atgaacctca ggggatgtga agttacccca gatgtaaaca tttcaggcca aaaatttaac
     1381 attaaactcc tgattccagt tgcagaaggc atgaatgaaa tctggcttcg ttgtgacaat
     1441 gaaaaacagt atgcacactg gatggcagcc tgcagattag cctccaaagg caagaccatg
     1501 gcggacagtt cttacaactt agaagttcag aatattcttt cctttctgaa gatgcagcat
     1561 ttaaacccag atcctcagtt aataccagag cagatcacga ctgatataac tcctgaatgt
     1621 ttggtgtctc cccgctatct aaaaaagtat aagaacaagc agataacagc gagaatcttg
     1681 gaggcccatc agaatgtagc tcagatgagt ctaattgaag ccaagatgag atttattcaa
     1741 gcttggcagt cactacctga atttggcatc actcacttca ttgcaaggtt ccaagggggc
     1801 aaaaaagaag aacttattgg aattgcatac aacagactga ttcggatgga tgccagcact
     1861 ggagatgcaa ttaaaacatg gcgtttcagc aacatgaaac agtggaatgt caactgggaa
     1921 atcaaaatgg tcaccgtaga gtttgcagat gaagtacgat tgtccttcat ttgtactgaa
     1981 gtagattgca aagtggttca tgaattcatt ggtggctaca tatttctctc aacacgtgca
     2041 aaagaccaaa acgagagttt agatgaagag atgttctaca aacttaccag tggttgggtg
     2101 ggtaactcag gggatccagg aatggtgagc aagggcgagg agctgttcac cggggtggtg
     2161 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2221 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2281 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2341 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2401 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2461 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2521 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2581 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2641 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2701 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2761 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2821 atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     2881 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2941 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat
//
