LOCUS       sequence-199519-        1582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To visualize membrane dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Artym VV, Matsumoto K, Mueller SC, Yamada KM
  TITLE     Dynamic membrane remodeling at invadopodia differentiates 
            invadopodia from podosomes.
  JOURNAL   Eur J Cell Biol. 2011 Feb-Mar;90(2-3):172-80. doi: 
            10.1016/j.ejcb.2010.06.006. Epub 2010 Jul 24.
  PUBMED    20656375
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J Cell 
            Biol. 2011 Feb-Mar"; volume: "90(2-3)"; pages: "172-80. doi: 
            10.1016/j.ejcb.2010.06.006. Epub 2010 Jul 24"
COMMENT     Sequence Label: pIL2RmCherry
FEATURES             Location/Qualifiers
     source          1..1582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             856..1566
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1001..1019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1269..1288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctagcgctac cggtcgccac catggattca tacctgctga tgtggggact gctcacgttc
       61 atcatggtgc ctggctgcca ggcagagctc tgtgacgatg acccgccaga gatcccacac
      121 gccacattca aagccatggc ctacaaggaa ggaaccatgt tgaactgtga atgcaagaga
      181 ggtttccgca gaataaaaag cgggtcactc tatatgctct gtacaggaaa ctctagccac
      241 tcgtcctggg acaaccaatg tcaatgcaca agctctgcca ctcggaacac aacgaaacaa
      301 gtgacacctc aacctgaaga acagaaagaa aggaaaacca cagaaatgca aagtccaatg
      361 cagccagtgg accaagcgag ccttccaggt cactgcaggg aacctccacc atgggaaaat
      421 gaagccacag agagaattta tcatttcgtg gtggggcaga tggtttatta tcagtgcgtc
      481 cagggataca gggctctaca cagaggtcct gctgagagcg tctgcaaaat gacccacggg
      541 aagacaaggt ggacccagcc ccagctcata tgcacaggtg aaatggagac cagtcagttt
      601 ccaggtgaag agaagcctca ggcaagcccc gaaggccgtc ctgagagtga gacttcctgc
      661 ctcgtcacaa caacagattt tcaaatacag acagaaatgg ctgcaaccat ggagacgtcc
      721 atatttacaa cagagtacca ggtagcagtg gccggctgtg ttttcctgct gatcagcgtc
      781 ctcctcctga gtgggctcac ctggtccgga ctcagatctc gagctgcggc cgccggatcc
      841 aagcttggag gtggaatggt gagcaagggc gaggaggata acatggccat catcaaggag
      901 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      961 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1021 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1081 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     1141 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1201 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1261 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     1321 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     1381 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1441 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     1501 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1561 aagtagtcta gataactgat ca
//
