LOCUS       sequence-199540-        6129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Improved FRET biosensor for the measurement of BCR-ABL activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Horiguchi M, Fujioka M, Kondo T, Fujioka Y, Li X, Horiuchi K, O 
            Satoh A, Nepal P, Nishide S, Nanbo A, Teshima T, Ohba Y
  TITLE     Improved FRET Biosensor for the Measurement of BCR-ABL Activity in 
            Chronic Myeloid Leukemia Cells.
  JOURNAL   Cell Struct Funct. 2017 Feb 2;42(1):15-26. doi: 10.1247/csf.16019. 
            Epub 2016 Dec 8.
  PUBMED    27928132
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1247/csf.16019";
            journalName: "Cell Struct Funct"; date: "2017-02-2- 2"; volume: 
            "42"; issue: "1"; pages: "15-26"
COMMENT     Sequence Label: pFX-Pickles 2.34NES
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(514..717)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(543..563)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        718..1021
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1196..1784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1276..1295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2113..2160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2185..2204
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2392..3186)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2392..3186)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2503..2522)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3113..3132
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3221..3578)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3235..3370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3387..3578
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     3535..3555
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(3580..3684)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3711..4166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3798..3817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4008..4029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4173..4294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4184..4203)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4238..4257
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(4451..4963,4964..4966,4967..4969))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             complement(join(6029..6129,1..412,413..415,416..418))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
ORIGIN
        1 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
       61 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      121 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      181 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg
      241 tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt
      301 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      361 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgc
      421 cgaattcgat atcaagacct gctaatttca aggctaattc attcatggcg tctagaaccg
      481 gtagcgctag cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg
      541 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      601 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      661 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      721 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      781 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      841 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      901 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      961 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     1021 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataacg
     1081 aatgcatggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1921 tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg
     1981 cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg
     2041 cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa
     2101 ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg
     2161 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag
     2221 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     2281 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     2341 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     2401 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     2461 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     2521 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     2581 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     2641 ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     2701 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     2761 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     2821 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     2881 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     2941 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     3001 gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     3061 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     3121 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     3181 aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc
     3241 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     3301 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     3361 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     3421 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
     3481 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
     3541 actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta
     3601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     3721 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3781 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3841 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3901 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3961 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4021 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4081 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4141 taacaaaata ttaacgctta caatttacgc cttaagatac attgatgagt ttggacaaac
     4201 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4261 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4321 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4381 tggtatggct gattatgatc agttatctag atccggtgga tcttcagtca gtcagcggcc
     4441 tccagatcta ctcgatgttg tggcggatct tgaagtgggc cttgatgccg ttcttctgct
     4501 tgtcggcggt gatatagacg ttgtggctga tgtagttgta ctccagcttg tgccccagga
     4561 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4621 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtac
     4681 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4741 cggggtagcg gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg
     4801 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     4861 cgccctcgcc ctcgccggac acgctgaacc tgtggccgtt tacgtcgccg tccagctcga
     4921 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatt cctccggatc
     4981 ctcccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     5041 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     5101 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     5161 cggcgagctg cacgctgccg tcgcggccgc gagaaccgga aactgcaggt agaggagttg
     5221 tggtctgagg ttgagcgtat gcatgagcag gttctgggat cccataactg ttggagttcc
     5281 tatttccatg ctttccgtgt ggtgaggatc tcacaagctt ttcgacataa gggacaggaa
     5341 tcatcccaac ccggccatcc ttgttccggg cactccacca ctgttcttca ggcttctcta
     5401 ttatcactag gatctcaccc tttttaaagg gcaggtcttc ggcatcattc ccaggaaaat
     5461 catacagagt ccgtacatat tccaggttat cttctgctgt aggcaggttg ggtgctgaga
     5521 cagatcccat tggtgggctt ggatacctgg gcgcaggctc gatgagggtg gtggtggcca
     5581 ggtagtggat cttgtaaaac tccagcaggg ccggcaaatg gtcaaattcc tggtccccga
     5641 tcttaaaacg gcggttgggc agcgagttga tgatgtagtg ggagacccgc gagttctcgg
     5701 acaccgacag cacatagtcc ccagggcagg tggaagaatc gcggacgagg aacataccgt
     5761 ggcgctggcc ctggagccgg gtctgcgcct cctggcgaga caccggcccc atataccagg
     5821 cggagcggtc cgaggagtcg aacctggcgg aggacatctc gagggcggcg gtcacgaact
     5881 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctaagcttg gactggtagc
     5941 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     6001 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
     6061 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
     6121 ccagcttgt
//
