LOCUS       sequence-199567-        2064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites in a viral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Pegard NC, Mardinly AR, Oldenburg IA, Sridharan S, Waller L, Adesnik
            H
  TITLE     Three-dimensional scanless holographic optogenetics with temporal 
            focusing (3D-SHOT).
  JOURNAL   Nat Commun. 2017 Oct 31;8(1):1228. doi: 10.1038/s41467-017-01031-3.
  PUBMED    29089483
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-31"; volume: "8(1):1228. doi"; pages: " 
            10.1038/s41467-017-01031-3"
COMMENT     Sequence Label: pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..2064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(250..960)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             complement(973..2022)
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE
                     WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI
                     AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY
                     FEWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV
                     SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK
                     LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE
                     FVEEEDEDTV"
     protein_bind    2032..2064
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccttagaatc tggtggcttc
       61 agggaagtcc gtggcacagc tgatgaagct gtcaatgctg gacatgctcc gcatgtcgat
      121 gacgccctcc gtgcgtgcgg gcagaggggc cacggggctg ggcatgctgc tcatctccag
      181 ctcctctgga ggcttgctct gcggggccat ctccttggtg ttgaggatgg gctgggactg
      241 ctctagggcc ttgtacagct cgtccatccc accaccaagc ccggcgaact tggcaactgc
      301 gtgttcgcgt tgtactacga acatttcatt gtcactttcc tctaaccttt ccaggcggtg
      361 atcaacggca tggataccgg gcatcttgat gttcccgacg gtcttttttg acctgtaagt
      421 tgttacgaaa gagcaagaca gatggccacc accatcaact ttcagtgcca tatgagtgta
      481 tcccctcaga ccaccatctg ctggatacat catctctgta ttaggctccc aacccttggt
      541 cttcttctgc atcacgggac cattggaggg aaagtttacc cctctgactt ggacgtggta
      601 aacgagacag ccatcctcaa ggctggtgtc ctgcatgacg gtgacgactc caccatcttc
      661 gtatctcgta actctttccc aagtaaaacc ctcaggaaag gactgtttaa agaaatcagg
      721 aatgcctttc gggtacttga taaaagtacg gctgccatac atgaacgacg tggcaagaat
      781 gtcaaaggca aatggcaggg gtcctccctc gatgactttg atcctcatgg tttgagttcc
      841 catgtacgga ttgccttctc cttcacctgt gcatttgaat tggtggccgt tgaccgaacc
      901 ttccatgacc accttcatac gcatattttc cttgatcagc tcttcgccct tagacaccat
      961 taccggtgcc gccactgtgt cctcgtcctc ctcctccacg aactcttcca cttccacctc
     1021 ctcgcctccg atctccatct tggtggtctt ccggatgtcg ccgtggatca ggatgtgctc
     1081 gtggatcttg atcctcaggt ggtgggccag gaaggtccaa aactccttgg cgatgatgtc
     1141 gcagatgctg tggccgatgc tgttggcgta agggctcagc ttcagcagtc cttctggtcc
     1201 cactgcccag aggattgggt agctgcccca agaggcgaag taagcgtagg ccatcagctt
     1261 cacgaccatg cggcaatggc ctttaggcac gctgtggttg gcttccacgt agcacttggc
     1321 ggcctggaag tacatgtagc cgccgtagat gcaagacacg atatacagca gccacttgag
     1381 ccaatcggta gccagtcctg cggccatgcc gaacacgatc attcccacgc aagacacgat
     1441 caggcccatg gtccgcttgc tgtagtcgtt cttcaggccg ctcaggttgc tcagtctgat
     1501 caggatcacg gggcaagaca gcagccactc gaagtagcgc aggcaatagg cgtggttgcc
     1561 ggtagacagg tacactgtgg cggggctgct gaactccttg aagatctcca gggtcacgaa
     1621 cagcacctcg acgcaacaga cgtagacctc ctcccaaccg caagtggcct tccaggcgct
     1681 gaagccgtag aatgtcagca gggcgatggc gatgctgaaa gcaatccact ggcagacctg
     1741 ggcgccgatc ttttctcctg gtgtgcctct gctgtgcagc caagcctgct tcttctccac
     1801 tcccacgacc aggtagccgc ctgtggcctc cacgaagcaa tatccgtatc cggcgtcgct
     1861 cagtccgtaa gaagggtcac accaccactc ggtgctgaag cactcgcctg taggtgtcat
     1921 tcctccacag cccatgtcct cgtggtggta ggggttaggc caagggttga tgcctccggc
     1981 ggcaaacaga gatctggtgg cgctgctgat cagctcagcc atggtgctag cataacttcg
     2041 tataaagtat cctatacgaa gtta
//
