LOCUS       sequence-199593-        1908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Pegard NC, Mardinly AR, Oldenburg IA, Sridharan S, Waller L, Adesnik
            H
  TITLE     Three-dimensional scanless holographic optogenetics with temporal 
            focusing (3D-SHOT).
  JOURNAL   Nat Commun. 2017 Oct 31;8(1):1228. doi: 10.1038/s41467-017-01031-3.
  PUBMED    29089483
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-31"; volume: "8(1):1228. doi"; pages: " 
            10.1038/s41467-017-01031-3"
COMMENT     Sequence Label: pCAGGS-Chronos-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..1908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             991..1701
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atggaaacag ccgccacaat gacccacgcc tttatctcag ccgtgcctag cgccgaagcc
       61 acaattagag gcctgctgag cgccgcagca gtggtgacac cagcagcaga cgctcacgga
      121 gaaacctcta acgccacaac agccggagcc gatcacggtt gcttccccca catcaaccac
      181 ggaaccgagc tgcagcacaa gatcgcagtg ggactccagt ggttcaccgt gatcgtggct
      241 atcgtgcagc tcatcttcta cggttggcac agcttcaagg ccacaaccgg ctgggaggag
      301 gtctacgtct gcgtgatcga gctcgtcaag tgcttcatcg agctgttcca cgaggtcgac
      361 agcccagcca cagtgtacca gaccaacgga ggagccgtga tttggctgcg gtacagcatg
      421 tggctcctga cttgccccgt gatcctgatc cacctgagca acctgaccgg actgcacgaa
      481 gagtacagca agcggaccat gaccatcctg gtgaccgaca tcggcaacat cgtgtggggg
      541 atcacagccg cctttacaaa gggccccctg aagatcctgt tcttcatgat cggcctgttc
      601 tacggcgtga cttgcttctt ccagatcgcc aaggtgtata tcgagagcta ccacaccctg
      661 cccaaaggcg tctgccggaa gatttgcaag atcatggcct acgtcttctt ctgctcttgg
      721 ctgatgttcc ccgtgatgtt catcgccgga cacgagggac tgggcctgat cacaccttac
      781 accagcggaa tcggccacct gatcctggat ctgatcagca agaacacttg gggcttcctg
      841 ggccaccacc tgagagtgaa gatccacgag cacatcctga tccacggcga catccggaag
      901 acaaccacca tcaacgtggc cggcgagaac atggagatcg agaccttcgt cgacgaggag
      961 gaggagggag gagtggcggc cgcaccggta atggtgtcta agggcgaaga gctgatcaag
     1021 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
     1081 acaggtgaag gagaaggcaa tccgtacatg ggaactcaaa ccatgaggat caaagtcatc
     1141 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
     1201 cgtactttta tcaagtaccc gaaaggcatt cctgatttct ttaaacagtc ctttcctgag
     1261 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcatgcag
     1321 gacaccagcc ttgaggatgg ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt
     1381 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
     1441 atgtatccag cagatggtgg tctgagggga tacactcata tggcactgaa agttgatggt
     1501 ggtggccatc tgtcttgctc tttcgtaaca acttacaggt caaaaaagac cgtcgggaac
     1561 atcaagatgc ccggtatcca tgccgttgat caccgcctgg aaaggttaga ggaaagtgac
     1621 aatgaaatgt tcgtagtaca acgcgaacac gcagttgcca agttcgccgg gcttggtggt
     1681 gggatggacg agctgtacaa ggccctagag cagtcccagc ccatcctcaa caccaaggag
     1741 atggccccgc agagcaagcc tccagaggag ctggagatga gcagcatgcc cagccccgtg
     1801 gcccctctgc ccgcacgcac ggagggcgtc atcgacatgc ggagcatgtc cagcattgac
     1861 agcttcatca gctgtgccac ggacttccct gaagccacca gattctaa
//
