LOCUS       sequence-199599-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Trpc2 deletion  mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: Trpc2-KO TV
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(69..85)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(94..127)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    132..593
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     primer_bind     complement(714..733)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 actgaggcaa aaaaacaaat aaaataaaat aaaataaaat aaataataat aatattaaaa
       61 agcaccatga tccactagtt ctagattaat taaataactt cgtatagcat acattatacg
      121 aagttataag cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat
      181 gtggaacaga ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca
      241 cagtacttag aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta
      301 tagatctata gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt
      361 aaattatgat atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa
      421 agagcaggca tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt
      481 caaaatgtag gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat
      541 tttagtggac atctttattg tttaatagat catcaatttc tgcagactta cagcggatcc
      601 cctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
      661 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
      721 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
      781 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
      841 acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag
//
