LOCUS       sequence-199601-       12834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12834)
  TITLE     Astsaturov Lab Plasmids
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLEX-ccdb
FEATURES             Location/Qualifiers
     source          1..12834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(971..2173)
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIMGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRVATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     protein_bind    2182..2200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    2314..2448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2616..3249
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3296..3421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3918..4151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4336..4380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4678..4795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4860..5234)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      5231..5240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5244..5262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5254..5301)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        5406..5709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5710..5913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5864..5884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5910..5934
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6032..6058
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6038..6064
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    6077..6201
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6310..6993
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(6376..6395)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7313..7618
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7497..7516
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7659..7783)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7803..7832
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7879..8451
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8046..8063)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8273..8292
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8454..9056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8953..8973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9063..9651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9116..9136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9534..9545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9912..10092
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10193..10210)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10199..10423
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10469..10897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10556..10575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10766..10787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10906..10926)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10911..11240
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11091..11226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11153..11172
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11289..12314
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11289..12314
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    12444..12565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12614..12630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12614..12630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12627..12649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12638..12654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12662..12668,12669..12686,12687..12692))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12707..12728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgacaga gtcacgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
      901 gaatcgggag cggcgatacc ctatgcatca agcttggtac cgagctcgga tcgttaatta
      961 attaacgatc cgcacttgtc tcctgtttac tcccctgagc ttgaggggtt aacatgaagg
     1021 tcatcgatag caggataata atacagtaaa acgctaaacc aataatccaa atccagccat
     1081 cccaaattgg tagtgaatga ttgtaaataa cagcaaacag taatgggcca ataacaccgg
     1141 ttgcattggt aaggctcacc aataatccct gtaaagcacc ttgctgatga ctctttgttt
     1201 ggatagacat cactccctgt aatgcaggta acgcgatccc accaccagcc aataaaatta
     1261 aaacagggaa aactaaccaa ccttcagata taaacgctaa aaaggcaaat gcactactat
     1321 ctgcaataaa tccgagcagt actgccgttt tttcgcccca tttagtggct actcttcctg
     1381 ccacaaaggc ttggaatact gagtgtaaaa gaccaagacc cgctaatgaa aagccaacca
     1441 tcatgctatt ccatccaaaa cgattttcgg taaatagcac ccacaccgtt gcgggaattt
     1501 ggcctatcaa ttgcgctgaa aaataaataa tcaacaaaat gggcatcgtt ttaaataaag
     1561 tgatgtatac cgaattcgat tgcgtctcaa cccctacttc ggtatctgta ttatcacgtg
     1621 tatttttggt ttcacggaac caaaacataa ccacaaggaa agtgacaata tttagcaacg
     1681 cagcgataaa aaagggacta tgcggtgaaa tctctcctgc aaaaccaccc ataataggcc
     1741 ccgctattaa accaagccca aaacttgccc ctaaccaacc gaaccacttc acgcgttgag
     1801 aggctgaggt ggtatcggca atgaccgatg ccgcgacagc cccagtagct cctgtgatcc
     1861 ctgaaagcaa acggcctaaa tacagcatcc aaagcgcact tgaaaaagcc agcaataagt
     1921 aatccagcga tgcgcctatt aatgacaaca acagcaccgg gcgccgacca aatcggtcag
     1981 acatttttcc aagccaagga gcaaagataa cctgcattaa cgcataaagt gcaagcaata
     2041 cgccaaagtg gttagcgatg tcttccgaag caataaattc acgtaataac gttggcaaga
     2101 ctggcatgat aaggccaatc cccatggcat cgagtaacgt aattaccaat gcgatctttg
     2161 tcgaactatt catttcactt ttctctatca ctgataggga gtattgtctc atgagcggat
     2221 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2281 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     2341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     2461 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     2521 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     2581 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     2641 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     2701 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     2761 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     2821 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     2881 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     2941 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     3001 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     3061 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3241 tctctagcag tagtagttca tgtcrkactt gaaagcgaaa gggaaaccag aggagctctc
     3301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     3781 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3841 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3901 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3961 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     4021 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     4081 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     4141 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     4201 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     4261 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     4321 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     4381 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     4441 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     4501 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     4561 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     4621 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     4681 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4741 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     4801 ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat
     4861 cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac
     4921 tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag
     4981 ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc
     5041 caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg
     5101 tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg
     5161 tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg
     5221 gtcaacttgg ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta
     5281 tgccgatgat taattgtcaa cacgtgctgc aggtccgagg ttctagacgt attaccgcca
     5341 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5701 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     5761 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5821 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     5881 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     5941 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     6001 actctagcct aggccgcgga ccatgggatc ctatccgtat gatgttcctg attatgctag
     6061 cctcgggtac cggatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     6121 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     6181 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgmcc actttacgcm
     6241 taagaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     6301 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     6361 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     6421 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     6481 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     6541 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     6601 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     6661 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     6721 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     6781 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     6841 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     6901 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggg
     6961 gggcgtaaac gccgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     7021 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     7081 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     7141 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     7201 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     7261 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
     7321 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     7381 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     7441 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     7501 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     7561 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     7621 gtcaggctcc cttatacaca gccagtctgc aggtcgatma wagtgamakg atatgttgtg
     7681 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     7741 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttcgatct agaatggcta
     7801 gcgaacaaaa gctcatatct gaagaggacc tatagaattc tcgagtctag agggcccgac
     7861 gcgtctggcc accggtcgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     7921 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     7981 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     8041 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     8101 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     8161 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     8221 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     8281 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     8341 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     8401 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggcca
     8461 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     8521 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     8581 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8641 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8701 tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc
     8761 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8821 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8881 ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga
     8941 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     9001 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtta
     9061 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9121 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9181 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9241 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9301 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9361 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9421 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9481 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9541 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9601 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     9661 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     9721 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     9781 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9841 aaaagaaaag aggggactgg aagggctaay ycwcagatcc tgcatataag cagctgcttt
     9901 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10081 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    10141 aatgaatatc agagagtgag aggccttgac attgtttaaa cccgctgatc agcctcgact
    10201 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10261 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    10321 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    10381 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    10441 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
    10501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10621 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10681 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10741 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10801 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10861 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    10921 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10981 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    11041 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    11101 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    11161 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    11221 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    11281 agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    11341 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    11401 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    11461 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    11521 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    11581 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    11641 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    11701 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    11761 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    11821 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    11881 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    11941 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    12001 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    12061 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    12121 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    12181 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    12241 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    12301 gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    12361 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    12421 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    12481 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12541 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    12601 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    12661 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    12721 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    12781 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgct
//
