LOCUS       sequence-199607-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway 
            cloning. Cub-PLV does not contain a start codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Grefen C, Lalonde S, Obrdlik P
  TITLE     Split-ubiquitin system for identifying protein-protein interactions 
            in membrane and full-length proteins.
  JOURNAL   Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 
            10.1002/0471142301.ns0527s41.
  PUBMED    18428659
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Neurosci."; date: "2007-10"; volume: "Chapter 5:Unit 5.27. doi"; 
            pages: " 10.1002/0471142301.ns0527s41"
COMMENT     Sequence Label: pMetYC-Dest
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..382
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     protein_bind    386..510
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(535..540,541..558,559..565)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             619..1278
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(685..704)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1620..1925
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1804..1823
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1966..2090)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2305..2478
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2479..2652
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             join(2671..2922,2923..3276)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3214..3235
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3295..3528
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3680..3699)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3724..3772
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4074..4093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4075..4093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4100..4117)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4100..4116)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4109..4131)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4873..6035
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      5198..6035
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(6397..7491)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7492..7897)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7917..7935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8003..8107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8108..8176,8177..8968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8326..8345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9139..9727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9628..9647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9881..9898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10015..10036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10051..10056,10057..10074,10075..10081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10089..10105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10094..10116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10113..10129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10113..10129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10150..10168
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggatgcaagg gttcgaatcc cttagctctc attatttttt gctttttctc ttgaggtcac
       61 atgatcgcaa aatggcaaat ggcacgtgaa gctgtcgata ttggggaact gtggtggttg
      121 gcaaatgact aattaagtta gtcaaggcgc catcctcatg aaaactgtgt aacataataa
      181 ccgaagtgtc gaaaaggtgg caccttgtcc aattgaacac gctcgatgaa aaaaataaga
      241 tatatataag gttaagtaaa gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc
      301 ttgtcttttc atctactatt tccttcgtgt aatacagggt cgtcagatac atagatacaa
      361 ttctattacc cccatccata caatcacaag tttgtacaaa aaagctgaac gagaaacgta
      421 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      481 ctgtaaaaca caacatatcc agtcatattg gcggccgcat taggcacccc aggctttaca
      541 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
      601 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      661 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      721 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      781 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
      841 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      901 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      961 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1021 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     1081 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1141 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1201 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1261 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     1321 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     1381 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     1441 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     1501 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     1561 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     1621 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     1681 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     1741 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     1801 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     1861 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     1921 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     1981 tgttgtgttt tacagcatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     2041 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gattggtggt
     2101 ggcggatccg gtggaggtgg atcaggtatc cctccagatc aacaaagatt gatctttgcc
     2161 ggtaagcagc tagaagacgg tagaacgctg tctgattaca acattcagaa ggagtccacc
     2221 ttacatcttg tgctaaggct aagaggtggt atgcacagat cagcttgcgg ccgcatggca
     2281 ggccttgcgc aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg
     2341 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
     2401 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
     2461 gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
     2521 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
     2581 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
     2641 caggcgccga aagtagacgc gagcggccgc atgaaagcgt taacggccag gcaacaagag
     2701 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     2761 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     2821 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggatccg tctgttgcag
     2881 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc acttctggcg
     2941 caacagcata ttgaaggtca ttatcaggtc gacccttcct tattcaagcc gaatgctgat
     3001 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggtgacttg
     3061 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     3121 gacgaggtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa
     3181 aatagcgagt ttaaaccaat tgtcgtagat cttcgtcagc agagcttcac cattgaaggg
     3241 ctggcggttg gggttattcg caacggcgac tggctggaat tcccggggat ctgggccccc
     3301 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg
     3361 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt
     3421 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag
     3481 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggta gggggcgcga
     3541 ccggacccgc atcccccgtc tgggttttcc cctcccgtcc ccggttcgta tccacaataa
     3601 acacgagcac atacattaca aaaacctgcg gttgtcgtct gattatttgg tggatccggg
     3661 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     3721 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     3781 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     3841 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     3901 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
     3961 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     4021 ggggtcaggt ccacgaccca agctggccgc caccgcggtg gagctccaat tcgccctata
     4081 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4141 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4201 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     4261 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata atcgctgggc
     4321 cattctcatg aagaatatct tgaatttatt gtcatattac tagttggtgt ggaagtccta
     4381 atatcggtga tcaatatagt ggttgacatg ctggctagtc aacattgagc cttttgatca
     4441 tgcaaatata ttacggtatt ttacaatcaa atatcaaact taactattga ctttataact
     4501 tatttaggtg gtaacattct tataaaaaag aaaaaaatta ctgcaaaaca gtactagctt
     4561 ttaacttgta tcctaggtta tctatgctgt ctcaccatag agaatattac ctatttcaga
     4621 atgtatgtcc atgattcgcc gggtaaatac atataataca caaatctggc ttaataaagt
     4681 ctataatata tctcataaag aagtgctaaa ttggctagtg ctatatattt ttaagaaaat
     4741 ttcttttgac taagtccata tcgactttgt aaaagttcac attagcatac atatattaca
     4801 cgagccagaa atagtaactt ttgcctaaat cacaaattgc aaaatttaat tgcttgcaaa
     4861 aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt
     4921 ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc
     4981 aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc
     5041 cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt
     5101 tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta
     5161 gagttcgatg ataagctgtc aaacatgaga attaattcca catgttaaaa tagtgaagga
     5221 gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc actagggtcc ggttaaacgg
     5281 atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga
     5341 acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg
     5401 tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt
     5461 aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa
     5521 gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc
     5581 cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc
     5641 gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac
     5701 cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc
     5761 tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag
     5821 gcttgcaggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     5881 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     5941 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     6001 ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg ccttccaacc
     6061 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac
     6121 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt
     6181 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac
     6241 tcatctccat gcagttggac gatcgatgat aagctgtcaa acatgagaat taattctacc
     6301 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     6361 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     6421 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     6481 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     6541 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt
     6601 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     6661 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     6721 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     6781 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     6841 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     6901 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     6961 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     7021 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     7081 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     7141 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     7201 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
     7261 cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct catctggaag
     7321 tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     7381 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     7441 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     7501 atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt
     7561 aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact
     7621 tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg
     7681 gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa ggtatatgcg
     7741 tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt ctgtgcgata
     7801 gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa gagattcgaa
     7861 ctcttgcatc ttacgatacc tgagtattcc cacagttaat tcttgaagac gaaagggcct
     7921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9841 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9901 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9961 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    10021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    10081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    10141 caagctcgaa attaaccctc actaaaggga acaaaagctg ggtacc
//
