LOCUS       sequence-199608-        8496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Grefen C, Lalonde S, Obrdlik P
  TITLE     Split-ubiquitin system for identifying protein-protein interactions 
            in membrane and full-length proteins.
  JOURNAL   Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 
            10.1002/0471142301.ns0527s41.
  PUBMED    18428659
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Neurosci."; date: "2007-10"; volume: "Chapter 5:Unit 5.27. doi"; 
            pages: " 10.1002/0471142301.ns0527s41"
COMMENT     Sequence Label: pNX32-Dest
FEATURES             Location/Qualifiers
     source          1..8496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    483..607
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        777..879
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             880..1539
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(946..965)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1881..2186
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2065..2084
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2227..2351)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2365..2454
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2487..2674
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2542..2566)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2701..2717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2701..2717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2714..2736)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2725..2741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2749..2755,2756..2773,2774..2779))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2794..2815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2932..2949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3103..3691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3183..3202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3862..4653,4654..4722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4485..4504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4723..4827)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4854..6197
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5817..5864)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6238..6256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6294..6316)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6416..6435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6456..6737
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6738..7412
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7515..7970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7652..7673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7864..7883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8096..8118
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8110..8127
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8111..8127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8150..8496,1..358)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcg
      361 agatgcagat tttcgtcaag actttgaccg gtaaaaccgg aacattggaa gttgaatctt
      421 ccgataccat cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggaatc cctggtgggt
      481 cgacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      541 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      601 cactatggcg gccgcgggtg atgctgccaa cttagcggcc gtaagttgcg atcacccgac
      661 gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct
      721 ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat
      781 cggcacgcaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      841 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      901 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      961 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1021 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1081 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1141 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1201 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1261 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     1321 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1381 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1441 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1501 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     1561 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     1621 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     1681 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     1741 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     1801 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     1861 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     1921 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     1981 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     2041 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     2101 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     2161 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     2221 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     2281 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     2341 tacaaagtgg ttggtggtgg cggataccca tacgatgttc ctgactatgc gggctatccc
     2401 tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta cgctgctcag
     2461 tgctgaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     2521 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     2581 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     2641 atgaggtcgc tcttattgac cacacctcta ccggcagcat gcaagcttgg cgtaatcatg
     2701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag
     4861 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     4921 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     4981 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca
     5041 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     5101 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     5161 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     5221 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     5281 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     5341 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     5401 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     5461 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     5521 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     5581 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     5641 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     5701 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg
     5761 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag
     5821 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa
     5881 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac
     5941 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggccat agtgcgtgtt
     6001 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
     6061 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
     6121 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
     6181 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat
     6241 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     6301 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     6361 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     6421 tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata tatataatat
     6481 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
     6541 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
     6601 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
     6661 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     6721 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     6781 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     6841 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     6901 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     6961 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     7021 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     7081 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     7141 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     7201 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
     7261 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
     7321 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
     7381 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
     7441 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7501 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     7621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     7681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     7741 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7801 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7861 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7921 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     7981 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8041 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8101 tcacgacgtt gtaaaacgac ggccagtgcc aagctttcgc gagctcgata tccttttgtt
     8161 gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat
     8221 tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata
     8281 gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca
     8341 ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc atcatcatat
     8401 cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa
     8461 cttcttttct ttttttttct tttctctctc ccccgt
//
