LOCUS       sequence-199617-       13397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETvr-2in1-NN
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1304..1428
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1453..1458,1459..1476,1477..1483)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1537..2196
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1603..1622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2538..2843
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2722..2741
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2884..3008)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3034..3063
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3064..3093
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3434..3778
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3866..3868,3869..3871,3872..4582)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3911..3932)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4172..4191)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4519..4540
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4602..4725
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4760..4765,4766..4783,4784..4790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4798..4814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4803..4825
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4822..4838
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4822..4838
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5099..5223)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5240..5266
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5270..5296
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5306..5332
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5649..5827
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6440..6692
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6797..6821
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7342..8133
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8379..8967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8868..8887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9121..9138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9153..9293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9219..9238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9379..9401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9637..9831
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9897..10970)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11399..12028)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13359..13383
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtatggtg agtaaaggtg aagagttgat
      601 taaagagaac atgcatatga agttatacat ggagggaact gtcaataatc accactttaa
      661 gtgtacatca gagggtgagg gaaagccata cgagggaacc caaactatga gaatcaaagt
      721 agtggaggga ggtcctcttc catttgcttt tgatatacta gcaacaagtt tcatgtatgg
      781 ttccaggacc ttcattaacc atactcaggg aatccctgac ttctttaaac agtcttttcc
      841 tgaaggtttt acatgggaga gggttaccac ttacgaggac ggtggagtct tgacagcaac
      901 ccaggacact tcattacaag atggatgcct aatatacaat gtgaaaatta ggggtgtgaa
      961 tttccctagt aacggaccag ttatgcagaa gaaaacacta ggttgggaag ctaatactga
     1021 aatgttgtac cctgccgacg gaggtttaga aggtagatcc gacatggccc ttaagctagt
     1081 cggaggtgga cacttgatct gtaactttaa aaccacatat aggtctaaga agccagcaaa
     1141 gaatctaaaa atgcctggtg tttactatgt ggaccataga ctagaaagga taaaagaagc
     1201 agacaaagaa acttacgtgg agcaacatga ggtcgccgtc gctaggtatt gcgacttacc
     1261 ttccaagcta ggtcacaaat tgaacagtaa cactagtcct agaacaagtt tgtacaaaaa
     1321 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1381 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1501 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1561 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1621 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1681 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1741 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1801 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1861 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1921 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1981 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2041 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2101 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2161 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2221 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2281 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2341 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2401 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2461 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2521 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2581 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2641 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2701 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2761 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2821 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2881 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2941 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttctatac
     3001 aaagttggtc aattgttagg cggtggatct ctcgagcaga agttgatctc agaggaggac
     3061 ctcgagcaga agttgatctc agaggaggac ttataagcgg ccatgctaga gtccgcaaaa
     3121 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     3181 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     3241 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     3301 ctaaaaccaa aatccagtga agggctcgca cgtgcggagc tccgtaccct ctgcaggtca
     3361 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3421 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3481 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     3541 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     3601 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3661 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3721 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacct
     3781 cgagcgcgcg tatttttaaa caattaccaa caacaacaac aaacaacaac aacattacat
     3841 tttacattct acaactacat ctagtatggt gagcaagggc gaggagctgt tcaccggggt
     3901 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3961 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     4021 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     4081 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4141 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4201 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4261 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4321 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     4381 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaggatcc
     4501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4561 cggcatggac gagctgtaca aaagtaacac tagtcaattg gcaactttgt ataataaagt
     4621 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     4681 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcgggctca
     4741 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4801 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacccc gtccctgcac
     4861 gcctgtcgtt caaccctgga agatccgcgt gtcccgagtt ctaacagtct ggccgtcgtt
     4921 ctgcagcgtc gcgattggga aaacccgggc gtgacccaac tgaatcgtct ggcggcccat
     4981 ccgccgtttg cgtcgtggcg taactcagaa gaagcccgta ccgaccgtcc gtctcaacaa
     5041 ctgcgctcct aacactataa aaagctgatg cagacggggc ttttttctgc aggtcgacca
     5101 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     5161 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     5221 ggtgtctagg ttaccagtat acccttatga tgttcctgat tatgctggtt acccttacga
     5281 tgttcctgat tacgcaggat ctgtataccc ttacgatgtt ccagattatg cctaagcggc
     5341 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     5401 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     5461 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     5521 tcaataaaat ttctaattcc taaaaccaaa atccagtgaa gggtagatca tgagcggaga
     5581 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
     5641 aactgacaga accgcaacgt tgaaggagcc actgagcgcg ggtttctgga gtttaatgag
     5701 ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca
     5761 tctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat
     5821 ccaattagag tctcatattc actctcaact cgatcgaggg gatctaccat gagtccagaa
     5881 aggagaccag cagatattag gagagcaacc gaagcagata tgccagcagt ttgcaccatt
     5941 gtgaaccatt acatcgagac ttctacagtt aattttagga ctgaacctca agagccacag
     6001 gaatggacag atgatctcgt gagattaagg gaaagatacc cttggcttgt tgctgaggtg
     6061 gatggagaag ttgcaggtat tgcttacgca ggaccttgga aggctagaaa cgcttatgat
     6121 tggactgctg agtctaccgt ttacgtgtca cctagacatc aaagaaccgg acttggttca
     6181 accttgtata ctcacctttt gaagtctctt gaagctcagg gattcaaatc tgttgtggct
     6241 gttatcggtt tgcctaatga tccaagtgtg agaatgcatg aagctctcgg atacgcacca
     6301 aggggtatgt taagagctgc tggatttaaa catggtaact ggcacgatgt tggtttctgg
     6361 cagttagatt tcagtttacc agttccacca agaccagtgc ttccagtgac cgagatttga
     6421 cccgggctag agtcaagcag atcgttcaaa catttggcaa taaagtttct taatattgaa
     6481 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     6541 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     6601 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     6661 atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgaagctgag ttaacgatgt
     6721 aagtcctcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     6781 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     6841 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     6901 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     6961 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     7021 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     7081 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     7141 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     7201 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     7261 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     7321 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     7381 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     7441 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     7501 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     7561 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     7621 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     7681 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     7741 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     7801 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     7861 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     7921 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     7981 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8041 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     8101 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     8161 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     8221 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     8281 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8521 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9121 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     9181 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     9241 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     9301 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     9361 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     9421 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     9481 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     9541 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     9601 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     9661 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     9721 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     9781 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     9841 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     9901 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     9961 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
    10021 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
    10081 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
    10141 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
    10201 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
    10261 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
    10321 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
    10381 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
    10441 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
    10501 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
    10561 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
    10621 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
    10681 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
    10741 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
    10801 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
    10861 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
    10921 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
    10981 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
    11041 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
    11101 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
    11161 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
    11221 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
    11281 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
    11341 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
    11401 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
    11461 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
    11521 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
    11581 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
    11641 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
    11701 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
    11761 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
    11821 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
    11881 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    11941 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    12001 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    12061 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    12121 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    12181 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    12241 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    12301 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    12361 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    12421 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    12481 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    12541 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    12601 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    12661 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    12721 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    12781 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    12841 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    12901 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    12961 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    13021 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    13081 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    13141 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    13201 tggatcaaag tactttaaag tactttaaag tactttaaag tactttgatc ccgaggggaa
    13261 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
    13321 aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
    13381 tcaaacactg atagttt
//
