LOCUS       sequence-199623-       13306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETvr-2in1-CN
FEATURES             Location/Qualifiers
     source          1..13306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1304..1428
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1453..1458,1459..1476,1477..1483)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1537..2196
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1603..1622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2538..2843
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2722..2741
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2884..3008)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3034..3063
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3064..3093
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3434..3778
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3873..3996
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4031..4036,4037..4054,4055..4061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4069..4085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4074..4096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4093..4109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4093..4109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4370..4494)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4514..4516,4517..4519,4520..5233)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4559..4580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4820..4839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5167..5188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5558..5736
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6349..6601
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6706..6730
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7251..8042
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8288..8876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8777..8796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9030..9047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9062..9202
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9128..9147)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9288..9310
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9546..9740
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9806..10879)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11308..11937)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13268..13292
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtatggtg agtaaaggtg aagagttgat
      601 taaagagaac atgcatatga agttatacat ggagggaact gtcaataatc accactttaa
      661 gtgtacatca gagggtgagg gaaagccata cgagggaacc caaactatga gaatcaaagt
      721 agtggaggga ggtcctcttc catttgcttt tgatatacta gcaacaagtt tcatgtatgg
      781 ttccaggacc ttcattaacc atactcaggg aatccctgac ttctttaaac agtcttttcc
      841 tgaaggtttt acatgggaga gggttaccac ttacgaggac ggtggagtct tgacagcaac
      901 ccaggacact tcattacaag atggatgcct aatatacaat gtgaaaatta ggggtgtgaa
      961 tttccctagt aacggaccag ttatgcagaa gaaaacacta ggttgggaag ctaatactga
     1021 aatgttgtac cctgccgacg gaggtttaga aggtagatcc gacatggccc ttaagctagt
     1081 cggaggtgga cacttgatct gtaactttaa aaccacatat aggtctaaga agccagcaaa
     1141 gaatctaaaa atgcctggtg tttactatgt ggaccataga ctagaaagga taaaagaagc
     1201 agacaaagaa acttacgtgg agcaacatga ggtcgccgtc gctaggtatt gcgacttacc
     1261 ttccaagcta ggtcacaaat tgaacagtaa cactagtcct agaacaagtt tgtacaaaaa
     1321 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1381 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1501 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1561 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1621 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1681 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1741 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1801 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1861 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1921 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1981 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2041 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2101 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2161 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2221 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2281 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2341 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2401 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2461 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2521 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2581 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2641 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2701 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2761 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2821 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2881 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2941 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttctatac
     3001 aaagttggtc aattgttagg cggtggatct ctcgagcaga agttgatctc agaggaggac
     3061 ctcgagcaga agttgatctc agaggaggac ttataagcgg ccatgctaga gtccgcaaaa
     3121 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     3181 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     3241 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     3301 ctaaaaccaa aatccagtga agggctcgca cgtgcggagc tccgtaccct ctgcaggtca
     3361 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3421 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3481 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     3541 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     3601 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3661 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3721 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacct
     3781 cgagcgcgcg tatttttaaa caattaccaa caacaacaac aaacaacaac aacattacat
     3841 tttacattct acaactacat ctagtcaatt ggcaactttg tataataaag ttgaacgaga
     3901 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     3961 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcgggctc actcattagg
     4021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4081 aacaatttca cacaggaaac agctatgacc atgattaccc cgtccctgca cgcctgtcgt
     4141 tcaaccctgg aagatccgcg tgtcccgagt tctaacagtc tggccgtcgt tctgcagcgt
     4201 cgcgattggg aaaacccggg cgtgacccaa ctgaatcgtc tggcggccca tccgccgttt
     4261 gcgtcgtggc gtaactcaga agaagcccgt accgaccgtc cgtctcaaca actgcgctcc
     4321 taacactata aaaagctgat gcagacgggg cttttttctg caggtcgacc atagtgactg
     4381 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     4441 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgtctag
     4501 gttaggcact agtatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4561 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4621 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
     4681 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
     4741 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4801 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4861 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4921 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
     4981 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
     5041 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5101 gcccgacaac cactacctga gctaccagtc caagctgagc aaggatccca acgagaagcg
     5161 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5221 gctgtacaag taattaaagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta
     5281 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     5341 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     5401 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     5461 gacctaggag ggtagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga
     5521 tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca
     5581 ctgagcgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
     5641 cgttcaaaag tcgcctaagg tcactatcat ctagcaaata tttcttgtca aaaatgctcc
     5701 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaactc
     5761 gatcgagggg atctaccatg agtccagaaa ggagaccagc agatattagg agagcaaccg
     5821 aagcagatat gccagcagtt tgcaccattg tgaaccatta catcgagact tctacagtta
     5881 attttaggac tgaacctcaa gagccacagg aatggacaga tgatctcgtg agattaaggg
     5941 aaagataccc ttggcttgtt gctgaggtgg atggagaagt tgcaggtatt gcttacgcag
     6001 gaccttggaa ggctagaaac gcttatgatt ggactgctga gtctaccgtt tacgtgtcac
     6061 ctagacatca aagaaccgga cttggttcaa ccttgtatac tcaccttttg aagtctcttg
     6121 aagctcaggg attcaaatct gttgtggctg ttatcggttt gcctaatgat ccaagtgtga
     6181 gaatgcatga agctctcgga tacgcaccaa ggggtatgtt aagagctgct ggatttaaac
     6241 atggtaactg gcacgatgtt ggtttctggc agttagattt cagtttacca gttccaccaa
     6301 gaccagtgct tccagtgacc gagatttgac ccgggctaga gtcaagcaga tcgttcaaac
     6361 atttggcaat aaagtttctt aatattgaat cctgttgccg gtcttgcgat gattatcata
     6421 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     6481 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     6541 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     6601 cgaccggcat gaagctgagt taacgatgta agtcctcaat tcggcgttaa ttcagtacat
     6661 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     6721 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     6781 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     6841 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     6901 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     6961 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     7021 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     7081 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     7141 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     7201 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
     7261 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7321 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7381 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     7441 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     7501 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     7561 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     7621 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     7681 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     7741 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     7801 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     7861 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     7921 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     7981 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     8041 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     8101 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     8161 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     8221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8401 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     8461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8941 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9001 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9061 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     9121 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     9181 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     9241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     9361 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     9421 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     9481 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     9541 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     9601 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     9661 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     9721 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     9781 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     9841 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     9901 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     9961 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
    10021 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
    10081 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
    10141 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
    10201 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
    10261 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
    10321 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
    10381 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
    10441 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
    10501 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
    10561 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
    10621 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
    10681 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
    10741 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
    10801 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
    10861 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    10921 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    10981 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    11041 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    11101 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    11161 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    11221 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    11281 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    11341 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    11401 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    11461 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    11521 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    11581 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    11641 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    11701 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    11761 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    11821 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    11881 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    11941 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    12001 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    12061 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    12121 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    12181 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    12241 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    12301 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    12361 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    12421 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    12481 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    12541 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    12601 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    12661 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    12721 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    12781 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    12841 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    12901 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    12961 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    13021 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    13081 agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt
    13141 actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    13201 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
    13261 ctcttaggtt tacccgccaa tatatcctgt caaacactga tagttt
//
