LOCUS       sequence-199625-       13356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETgc-2in1-CN
FEATURES             Location/Qualifiers
     source          1..13356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        165..509
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             597..1304
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1010..1029
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1323..1447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1472..1477,1478..1495,1496..1502)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1556..2215
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1622..1641)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2557..2862
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2741..2760
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2903..3027)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3053..3082
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3083..3112
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3486..3830
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3925..4048
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4083..4088,4089..4106,4107..4113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4121..4137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4126..4148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4145..4161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4145..4161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4422..4546)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4566..4568,4569..4571,4572..5288)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKL"
     primer_bind     complement(4611..4632)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4872..4891)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5219..5240
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5608..5786
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6399..6651
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6756..6780
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7301..8092
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8338..8926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8827..8846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9080..9097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9112..9252
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9178..9197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9338..9360
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9596..9790
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9856..10929)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11358..11987)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13318..13342
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcaacg
       61 acggccagtg aattcaggcc tctgcaggtc aacatggtgg agcacgacac tctcgtctac
      121 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
      181 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      241 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
      301 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
      361 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
      421 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
      481 taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa acaattacca
      541 acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca tctagtatgg
      601 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      661 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      721 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      781 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      841 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      901 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      961 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1021 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1081 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     1201 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagtaac actagtccta
     1321 gaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
     1381 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
     1441 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     1501 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
     1561 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1621 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1681 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1741 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1801 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1861 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1921 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1981 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2041 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2101 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     2161 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     2221 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     2281 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     2341 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     2401 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     2461 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     2521 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     2581 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     2641 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     2701 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2761 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2821 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2881 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2941 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     3001 tcgttcaact tttctataca aagttggtca attgttaggc ggtggatctc tcgagcagaa
     3061 gttgatctca gaggaggacc tcgagcagaa gttgatctca gaggaggact tataagcggc
     3121 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     3181 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     3241 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     3301 tcaataaaat ttctaattcc taaaaccaaa atccagtgaa ggcctaagct tggcgtaatc
     3361 atggtcatag ctgttgctcg cacgtgcgga gctccgtacc ctctgcaggt caacatggtg
     3421 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg
     3481 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     3541 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     3601 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat
     3661 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     3721 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3781 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgagcgcg
     3841 cgtattttta aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt
     3901 ctacaactac atctagtcaa ttggcaactt tgtataataa agttgaacga gaaacgtaaa
     3961 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     4021 gtaaaacaca acatatccag tcactatggc ggccgcgggc tcactcatta ggcaccccag
     4081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4141 cacacaggaa acagctatga ccatgattac cccgtccctg cacgcctgtc gttcaaccct
     4201 ggaagatccg cgtgtcccga gttctaacag tctggccgtc gttctgcagc gtcgcgattg
     4261 ggaaaacccg ggcgtgaccc aactgaatcg tctggcggcc catccgccgt ttgcgtcgtg
     4321 gcgtaactca gaagaagccc gtaccgaccg tccgtctcaa caactgcgct cctaacacta
     4381 taaaaagctg atgcagacgg ggcttttttc tgcaggtcga ccatagtgac tggatatgtt
     4441 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     4501 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgtct aggttaggca
     4561 ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4621 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4681 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     4741 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     4801 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4861 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4921 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4981 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     5041 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     5101 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5161 accactacct gagtactcag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     5221 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5281 agttataagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     5341 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     5401 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     5461 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctaggag
     5521 ggtagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
     5581 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagcgcgg
     5641 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     5701 tcgcctaagg tcactatcat ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     5761 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaactc gatcgagggg
     5821 atctaccatg agtccagaaa ggagaccagc agatattagg agagcaaccg aagcagatat
     5881 gccagcagtt tgcaccattg tgaaccatta catcgagact tctacagtta attttaggac
     5941 tgaacctcaa gagccacagg aatggacaga tgatctcgtg agattaaggg aaagataccc
     6001 ttggcttgtt gctgaggtgg atggagaagt tgcaggtatt gcttacgcag gaccttggaa
     6061 ggctagaaac gcttatgatt ggactgctga gtctaccgtt tacgtgtcac ctagacatca
     6121 aagaaccgga cttggttcaa ccttgtatac tcaccttttg aagtctcttg aagctcaggg
     6181 attcaaatct gttgtggctg ttatcggttt gcctaatgat ccaagtgtga gaatgcatga
     6241 agctctcgga tacgcaccaa ggggtatgtt aagagctgct ggatttaaac atggtaactg
     6301 gcacgatgtt ggtttctggc agttagattt cagtttacca gttccaccaa gaccagtgct
     6361 tccagtgacc gagatttgac ccgggctaga gtcaagcaga tcgttcaaac atttggcaat
     6421 aaagtttctt aatattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     6481 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     6541 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     6601 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgaccggcat
     6661 gaagctgagt taacgatgta agtcctcaat tcggcgttaa ttcagtacat taaaaacgtc
     6721 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     6781 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     6841 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     6901 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     6961 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     7021 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     7081 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     7141 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     7201 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     7261 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     7321 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     7381 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     7441 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     7501 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     7561 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     7621 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     7681 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     7741 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     7801 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     7861 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     7921 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     7981 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     8041 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     8101 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     8161 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     8221 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     8281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8461 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8761 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8821 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8881 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8941 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9001 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9061 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     9121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     9181 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     9241 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9301 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9361 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     9421 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     9481 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     9541 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     9601 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     9661 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     9721 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     9781 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     9841 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9901 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9961 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
    10021 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
    10081 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
    10141 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
    10201 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
    10261 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
    10321 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
    10381 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
    10441 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
    10501 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
    10561 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
    10621 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
    10681 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
    10741 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
    10801 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
    10861 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10921 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    10981 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    11041 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    11101 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    11161 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    11221 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    11281 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    11341 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    11401 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    11461 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    11521 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    11581 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    11641 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    11701 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    11761 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    11821 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    11881 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    11941 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    12001 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    12061 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    12121 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    12181 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    12241 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    12301 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    12361 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    12421 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    12481 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    12541 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    12601 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    12661 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    12721 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    12781 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    12841 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    12901 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    12961 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    13021 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    13081 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    13141 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
    13201 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    13261 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    13321 tacccgccaa tatatcctgt caaacactga tagttt
//
