LOCUS       sequence-199626-       13240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETvr-2in1-CC
FEATURES             Location/Qualifiers
     source          1..13240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    581..705
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(730..735,736..753,754..760)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             814..1473
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(880..899)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1815..2120
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1999..2018
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2161..2285)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3368..3712
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3807..3930
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3965..3970,3971..3988,3989..3995)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4003..4019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4008..4030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4027..4043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4027..4043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4304..4428)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4448..4450,4451..4453,4454..5167)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4493..4514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4754..4773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5101..5122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5492..5670
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6283..6535
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6640..6664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7185..7976
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8222..8810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8711..8730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8964..8981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8996..9136
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9062..9081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9222..9244
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9480..9674
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9740..10813)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11242..11871)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13202..13226
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtcctaga acaagtttgt acaaaaaagc
      601 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      661 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
      721 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      781 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      841 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      901 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      961 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1021 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1081 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1141 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1201 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1261 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1321 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1381 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1441 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     1501 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     1561 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     1621 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     1681 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     1741 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     1801 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     1861 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     1921 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     1981 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     2041 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     2101 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     2161 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2221 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa
     2281 gttggtcaat tgttaggcac tagtatggtg agtaaaggtg aagagttgat taaagagaac
     2341 atgcatatga agttatacat ggagggaact gtcaataatc accactttaa gtgtacatca
     2401 gagggtgagg gaaagccata cgagggaacc caaactatga gaatcaaagt agtggaggga
     2461 ggtcctcttc catttgcttt tgatatacta gcaacaagtt tcatgtatgg ttccaggacc
     2521 ttcattaacc atactcaggg aatccctgac ttctttaaac agtcttttcc tgaaggtttt
     2581 acatgggaga gggttaccac ttacgaggac ggtggagtct tgacagcaac ccaggacact
     2641 tcattacaag atggatgcct aatatacaat gtgaaaatta ggggtgtgaa tttccctagt
     2701 aacggaccag ttatgcagaa gaaaacacta ggttgggaag ctaatactga aatgttgtac
     2761 cctgccgacg gaggtttaga aggtagatcc gacatggccc ttaagctagt cggaggtgga
     2821 cacttgatct gtaactttaa aaccacatat aggtctaaga agccagcaaa gaatctaaaa
     2881 atgcctggtg tttactatgt ggaccataga ctagaaagga taaaagaagc agacaaagaa
     2941 acttacgtgg agcaacatga ggtcgccgtc gctaggtatt gcgacttacc ttccaagcta
     3001 ggtcacaaat tgaactgatt ataagcggcc atgctagagt ccgcaaaaat caccagtctc
     3061 tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat
     3121 aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     3181 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     3241 tccagtgacc taggagggct cgcacgtgcg gagctccgta ccctctgcag gtcaacatgg
     3301 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaga
     3361 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     3421 cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc tacaaatgcc
     3481 atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt ggtcccaaag
     3541 atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     3601 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     3661 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acctcgagcg
     3721 cgcgtatttt taaacaatta ccaacaacaa caacaaacaa caacaacatt acattttaca
     3781 ttctacaact acatctagtc aattggcaac tttgtataat aaagttgaac gagaaacgta
     3841 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3901 ctgtaaaaca caacatatcc agtcactatg gcggccgcgg gctcactcat taggcacccc
     3961 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     4021 ttcacacagg aaacagctat gaccatgatt accccgtccc tgcacgcctg tcgttcaacc
     4081 ctggaagatc cgcgtgtccc gagttctaac agtctggccg tcgttctgca gcgtcgcgat
     4141 tgggaaaacc cgggcgtgac ccaactgaat cgtctggcgg cccatccgcc gtttgcgtcg
     4201 tggcgtaact cagaagaagc ccgtaccgac cgtccgtctc aacaactgcg ctcctaacac
     4261 tataaaaagc tgatgcagac ggggcttttt tctgcaggtc gaccatagtg actggatatg
     4321 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     4381 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtgt ctaggttagg
     4441 cactagtatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4501 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4561 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     4621 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     4681 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4741 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4801 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4861 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     4921 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     4981 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5041 caaccactac ctgagctacc agtccaagct gagcaaggat cccaacgaga agcgcgatca
     5101 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5161 caagtaatta aagcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc
     5221 tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg
     5281 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     5341 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgaccta
     5401 ggagggtaga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     5461 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     5521 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     5581 aaagtcgcct aaggtcacta tcatctagca aatatttctt gtcaaaaatg ctccactgac
     5641 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga
     5701 ggggatctac catgagtcca gaaaggagac cagcagatat taggagagca accgaagcag
     5761 atatgccagc agtttgcacc attgtgaacc attacatcga gacttctaca gttaatttta
     5821 ggactgaacc tcaagagcca caggaatgga cagatgatct cgtgagatta agggaaagat
     5881 acccttggct tgttgctgag gtggatggag aagttgcagg tattgcttac gcaggacctt
     5941 ggaaggctag aaacgcttat gattggactg ctgagtctac cgtttacgtg tcacctagac
     6001 atcaaagaac cggacttggt tcaaccttgt atactcacct tttgaagtct cttgaagctc
     6061 agggattcaa atctgttgtg gctgttatcg gtttgcctaa tgatccaagt gtgagaatgc
     6121 atgaagctct cggatacgca ccaaggggta tgttaagagc tgctggattt aaacatggta
     6181 actggcacga tgttggtttc tggcagttag atttcagttt accagttcca ccaagaccag
     6241 tgcttccagt gaccgagatt tgacccgggc tagagtcaag cagatcgttc aaacatttgg
     6301 caataaagtt tcttaatatt gaatcctgtt gccggtcttg cgatgattat catataattt
     6361 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     6421 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     6481 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaccg
     6541 gcatgaagct gagttaacga tgtaagtcct caattcggcg ttaattcagt acattaaaaa
     6601 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     6661 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     6721 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6781 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6841 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6901 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     6961 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     7021 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     7081 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     7141 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     7201 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     7261 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     7321 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     7381 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     7441 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     7501 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     7561 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     7621 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     7681 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     7741 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     7801 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     7861 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     7921 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     7981 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     8041 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     8101 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     8161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8341 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8881 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8941 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     9001 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     9061 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     9121 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9181 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     9241 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     9301 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     9361 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     9421 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     9481 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     9541 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     9601 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     9661 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     9721 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     9781 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     9841 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     9901 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     9961 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
    10021 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
    10081 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
    10141 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
    10201 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    10261 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    10321 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    10381 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    10441 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    10501 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    10561 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    10621 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    10681 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    10741 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    10801 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    10861 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    10921 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    10981 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    11041 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    11101 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    11161 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    11221 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    11281 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    11341 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    11401 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    11461 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    11521 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    11581 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    11641 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    11701 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    11761 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    11821 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    11881 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    11941 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    12001 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    12061 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    12121 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    12181 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    12241 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    12301 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    12361 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    12421 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    12481 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    12541 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    12601 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    12661 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    12721 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    12781 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    12841 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    12901 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    12961 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    13021 ctgcactata gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta
    13081 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    13141 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
    13201 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
//
