LOCUS       sequence-199628-       13297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13297)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETgc-2in1-CC
FEATURES             Location/Qualifiers
     source          1..13297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        165..509
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    603..727
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(752..757,758..775,776..782)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             836..1495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(902..921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1837..2142
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2021..2040
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2183..2307)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2327..3037
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2740..2759
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3427..3771
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3866..3989
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4024..4029,4030..4047,4048..4054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4062..4078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4067..4089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4086..4102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4086..4102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4363..4487)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4507..4509,4510..4512,4513..5229)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKL"
     primer_bind     complement(4552..4573)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4813..4832)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5160..5181
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5549..5727
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6340..6592
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6697..6721
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7242..8033
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8279..8867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8768..8787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9021..9038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9053..9193
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9119..9138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9279..9301
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9537..9731
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9797..10870)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11299..11928)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13259..13283
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcaacg
       61 acggccagtg aattcaggcc tctgcaggtc aacatggtgg agcacgacac tctcgtctac
      121 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
      181 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      241 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
      301 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
      361 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
      421 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
      481 taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa acaattacca
      541 acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca tctagtccta
      601 gaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      661 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      721 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      781 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
      841 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      901 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      961 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1021 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1081 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1141 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1201 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1261 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     1321 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     1381 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     1441 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     1501 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1561 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1621 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1681 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1741 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1801 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1861 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1921 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1981 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2041 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2101 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2161 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2221 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     2281 tcgttcaact tttctataca aagttggtca attgttaggc actagtatgg tgagcaaggg
     2341 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2401 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2461 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2521 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2581 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2641 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     2701 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2761 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     2821 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     2881 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2941 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3001 ccactccacc ggcggcatgg acgagctgta caagtaatta taagcggcca tgctagagtc
     3061 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     3121 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     3181 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     3241 ctaattccta aaaccaaaat ccagtgacct aggaggcctg catgcaagct tggcgtaatc
     3301 atggtcatag ctgttaactc gcacgtgcgg agctccgtac cctctgcagg tcaacatggt
     3361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
     3421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     3481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
     3541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
     3601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     3661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     3721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgagcgc
     3781 gcgtattttt aaacaattac caacaacaac aacaaacaac aacaacatta cattttacat
     3841 tctacaacta catctagtca attggcaact ttgtataata aagttgaacg agaaacgtaa
     3901 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     3961 tgtaaaacac aacatatcca gtcactatgg cggccgcggg ctcactcatt aggcacccca
     4021 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     4081 tcacacagga aacagctatg accatgatta ccccgtccct gcacgcctgt cgttcaaccc
     4141 tggaagatcc gcgtgtcccg agttctaaca gtctggccgt cgttctgcag cgtcgcgatt
     4201 gggaaaaccc gggcgtgacc caactgaatc gtctggcggc ccatccgccg tttgcgtcgt
     4261 ggcgtaactc agaagaagcc cgtaccgacc gtccgtctca acaactgcgc tcctaacact
     4321 ataaaaagct gatgcagacg gggctttttt ctgcaggtcg accatagtga ctggatatgt
     4381 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4441 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgtc taggttaggc
     4501 actagtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4561 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4621 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4681 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     4741 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4801 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4861 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4921 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4981 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5041 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5101 aaccactacc tgagtactca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     5161 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5221 aagttataag cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
     5281 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     5341 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     5401 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctagga
     5461 gggtagatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     5521 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagcgcg
     5581 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
     5641 gtcgcctaag gtcactatca tctagcaaat atttcttgtc aaaaatgctc cactgacgtt
     5701 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaact cgatcgaggg
     5761 gatctaccat gagtccagaa aggagaccag cagatattag gagagcaacc gaagcagata
     5821 tgccagcagt ttgcaccatt gtgaaccatt acatcgagac ttctacagtt aattttagga
     5881 ctgaacctca agagccacag gaatggacag atgatctcgt gagattaagg gaaagatacc
     5941 cttggcttgt tgctgaggtg gatggagaag ttgcaggtat tgcttacgca ggaccttgga
     6001 aggctagaaa cgcttatgat tggactgctg agtctaccgt ttacgtgtca cctagacatc
     6061 aaagaaccgg acttggttca accttgtata ctcacctttt gaagtctctt gaagctcagg
     6121 gattcaaatc tgttgtggct gttatcggtt tgcctaatga tccaagtgtg agaatgcatg
     6181 aagctctcgg atacgcacca aggggtatgt taagagctgc tggatttaaa catggtaact
     6241 ggcacgatgt tggtttctgg cagttagatt tcagtttacc agttccacca agaccagtgc
     6301 ttccagtgac cgagatttga cccgggctag agtcaagcag atcgttcaaa catttggcaa
     6361 taaagtttct taatattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     6421 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     6481 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     6541 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca
     6601 tgaagctgag ttaacgatgt aagtcctcaa ttcggcgtta attcagtaca ttaaaaacgt
     6661 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     6721 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     6781 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     6841 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     6901 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     6961 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     7021 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     7081 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     7141 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     7201 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     7261 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     7321 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     7381 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     7441 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     7501 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     7561 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     7621 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     7681 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     7741 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     7801 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     7861 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     7921 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     7981 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     8041 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     8101 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     8161 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     8221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8941 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9001 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     9061 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9121 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     9181 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9241 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9301 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     9361 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     9421 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     9481 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     9541 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     9601 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     9661 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     9721 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     9781 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     9841 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     9901 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     9961 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    10021 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    10081 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    10141 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    10201 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    10261 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    10321 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    10381 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    10441 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    10501 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    10561 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    10621 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    10681 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    10741 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    10801 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    10861 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    10921 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    10981 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    11041 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    11101 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    11161 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    11221 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    11281 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    11341 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    11401 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    11461 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    11521 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    11581 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    11641 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    11701 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    11761 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    11821 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    11881 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    11941 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    12001 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    12061 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    12121 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    12181 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    12241 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    12301 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    12361 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    12421 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    12481 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    12541 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    12601 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    12661 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    12721 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    12781 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    12841 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    12901 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    12961 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    13021 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    13081 cactatagca gcggaggggt tggatcaaag tactttaaag tactttaaag tactttaaag
    13141 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    13201 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
    13261 ttacccgcca atatatcctg tcaaacactg atagttt
//
