LOCUS       sequence-199633-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Gucy1b2-probe2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(926..944)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(927..944)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(962..978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(962..978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(975..997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gtcgcatgct cccggccgcc atggcggccg cgggaattcg attcagcgat gttgtgacat
       61 tcaccaacat ctgtgcggcc tgtgaaccta tccaaatagt gaacatgctc aactcaatgt
      121 actccaagtt tgacaggtta accaacatcc atgatgtcta taaagtagaa acaataggtg
      181 atgcttacat ggtggtaggt ggagtaccag tacccgttga aagccatgct caaagagttg
      241 ctaattttgc tctggggatg agaatttctg caaaagaagt gatgaatcct gtcactgggg
      301 aacccataca gatcagagtg ggaatccata ctggaccagt cttagcaggt gttgtgggag
      361 acaagatgcc ccggtactgc ctgtttggtg acaccgtaaa cacggcctct aggatggaaa
      421 gtcacgggct tcccaacaaa gtgcatctga gccccacagc ccacagggct ctggaaaaca
      481 aagggtttga aattgtcacg agaggggaga tagaggtgaa ggggaaagga aaaatgacca
      541 catacttcct gatccggaac ctgaatgcca ccgaggttga gatcatgggg cgaccttcag
      601 ctctcgctga tgggaaggaa gcaagtactc ctagaaacca agtcaagaag ccccgtgctg
      661 tcctgagcaa catggaccac caccagcagc aagtctacaa cagtgatcca gcagacgctt
      721 ctgatgaagt cacacttgct gggagtccag tggcagggcg agacgacaca gatgcagtca
      781 atagccagtc atcaccagat gagaccaaga caagtgcctt tgccagtgac cctgtgttgt
      841 ctgccaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa
      901 cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat
      961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
//
