LOCUS       sequence-199635-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Bleymehl K, Perez-Gomez A, Omura M, Moreno-Perez A, Macias D, Bai Z,
            Johnson RS, Leinders-Zufall T, Zufall F, Mombaerts P
  TITLE     A Sensor for Low Environmental Oxygen in the Mouse Main Olfactory 
            Epithelium.
  JOURNAL   Neuron. 2016 Dec 21;92(6):1196-1203. doi: 
            10.1016/j.neuron.2016.11.001. Epub 2016 Dec 1.
  PUBMED    27916458
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.11.001"; journalName: "Neuron"; date: 
            "2016-12-21- 21"; volume: "92"; issue: "6"; pages: "1196-1203"
COMMENT     Sequence Label: pISH-Gucy1b2-B
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(721..739)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(722..739)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(757..773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(757..773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(770..792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    781..797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgctcccggc cgccatggcg gccgcgggaa ttcgattgag aattcgagac gtgtaccatc
       61 cttttcagcg atgttgtgac attcaccaac atctgtgcgg cctgtgaacc tatccaaata
      121 gtgaacatgc tcaactcaat gtactccaag tttgacaggt taaccaacat ccatgatgtc
      181 tataaagtag aaacaatagg tgatgcttac atggtggtag gtggagtacc agtacccgtt
      241 gaaagccatg ctcaaagagt tgctaatttt gctctgggga tgagaatttc tgcaaaagaa
      301 gtgatgaatc ctgtcactgg ggaacccata cagatcagag tgggaatcca tactggacca
      361 gtcttagcag gtgttgtggg agacaagatg ccccggtact gcctgtttgg tgacaccgta
      421 aacacggcct ctaggatgga aagtcacggg cttcccaaca aagtgcatct gagccccaca
      481 gcccacaggg ctctggaaaa caaagggttt gaaattgtca cgagagggga gatagaggtg
      541 aaggggaaag gaaaaatgac cacatacttc ctgatccgga acctgaatgc caccgaggtt
      601 gagatcatgg ggcgaccttc agctctcgct gatgggaagg aatcactagt gaattcgcgg
      661 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
      721 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      781 ttgttatccg ctcacaattc cacacaacat a
//
