LOCUS       sequence-199638-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Gucy2d-1
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(861..879)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(862..879)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(897..913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(897..913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(910..932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    921..937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgctcccgg ccgccatggc cgcgggattc tcaggtctgc agcagggctg tcactttgaa
       61 ggccagaact ggacagcacc acactggaag acctgcctgc cctgccaggg cccgtggaga
      121 ctcactgtca gtcatctgaa gactgtctct tccatatcag tcctctctgt ggtattttgg
      181 agtgtcctac tttgggctga ctccctttcc ctgttagcat gggcaagaga gaccttcacc
      241 cttggggtgc tgggtccctg ggactgtgac cccatctttg cccaggccct ccccagcata
      301 gctacccagc tggctgtaga tcaagttaat caggatgcct cactgctgcc aggctcacag
      361 ttggatttca aggtcctccc cacaggctgt gacactcctc atgccctggc cacattcgtg
      421 gcccacaaga acatagtggc tgcttttgta ggccctgtta atcctgggtt ctgctcagca
      481 gcagccctgc tggcccaagg ctggggcaag tccctcttct cctgggcctg tgaagctcca
      541 gagggaggag gtgacttagt gcccacttta ccttctgcgg ccgatgtgct actgtctgtc
      601 atgagacact ttggctgggc tcgctgggcc attgtgtcct ctcaccagga catctgggta
      661 accacagccc agcagctggc cactgctttc aggactcatg ggctgcccat cggactggtg
      721 acctctttgg gacctggaga gaagggggcc acagaggttt gcaagcagct ccacaggttc
      781 atggtcaatc actagtgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt
      841 tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
      901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagcccgg
      961 aagcataaag t
//
