LOCUS       sequence-199639-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Gucy2d-2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(713..731)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(714..731)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(749..765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(749..765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(762..784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    773..789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcatgctccc ggccgccatg gcggccgcgg gaattcgatt tcgagaacag catcaaacag
       61 cgtgtagaga tcattgagga agcccaccac ctcaatgggc tcactcaagg ctgagatggt
      121 ggtgaagccc acgatgtcac tgaaatagat ggtgacctgg tcgaagtact cgggctccac
      181 tgttgtcccc atctttagag catgggcgac agacggaggg agcatctgcg agagcagcct
      241 ctctgtcttc cgcctctcca gttccagctc ctcagtccgc tcctggacca gatcttccag
      301 gctttcggaa tacttctcca gcatccgcag catggagtct acaacacttg tcttcttgcc
      361 ttggttgatg cttttgaact gtgtgtagat ctggtccaag cttggcctgt catctggagc
      421 ttcctcccag catagctgca tcaactggat acactcgagg ggaccctggt caggggacac
      481 cagtggccgg cacagaggag ggggagatgc caccttccgg atgatctctt ctgctgagag
      541 tccccaggag cagtagggtg ggtctcgggt cagtacctcc tgcaggatga tggccaagct
      601 gaaaacatca cctttgaagg tggccttccc agaatcacta gtgaattcgc ggccgcctgc
      661 aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt
      721 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      781 cgctcacaat tccacacaac
//
