LOCUS       sequence-199680-       10506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd9
FEATURES             Location/Qualifiers
     source          1..10506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..1262
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          315..1253
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1210..1230
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1282..1306
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3179..3313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3403..3427)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3435..3476
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3527..4115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3580..3600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3998..4009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4187..4420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4498..4619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4659..4794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4815..4833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4843..4860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4843..4859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4852..4874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5001..5456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5088..5107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5298..5319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5482..5586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5587..5655,5656..6447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5805..5824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6618..7206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7107..7126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7360..7377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7494..7515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7530..7535,7536..7553,7554..7560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7568..7584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7573..7595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7592..7608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7592..7608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7627..7647
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7629..7647
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7675..7901
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7902..8082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8129..8254
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8747..8980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9165..9209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9440..9650
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9462..9658
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9509..9644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9690..10292
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9690..9709)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10186..10206
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10335..10452
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
       61 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
      121 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      181 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      241 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      301 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
      361 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
      421 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
      481 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
      541 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
      601 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
      661 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
      721 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
      781 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
      841 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
      901 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
      961 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     1021 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     1081 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     1141 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     1201 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     1261 gaggctagca tcgattgatc aacaagtttg tacaaaaaag caggctggcg ccggaaccaa
     1321 ttcagtcgac tggatccggt accgaattca ggccggccta ccatggccgt gccaccgctg
     1381 ctccgcgggg cgctgctgct gtggcagctg ctggcgacgg gcggcgcggc gctggagatc
     1441 ggccgcttcg atccggagcg tggccgtggc cccgcgccgt gccaagcgat ggagatcccc
     1501 atgtgccggg gcattggcta caacctgacc cgcatgccca acctactggg ccacacgtcg
     1561 cagggcgagg cggcggcgca gctggccgag ttctcgcccc tcgtgcagta cggctgccac
     1621 agccacctgc gcttcttcct ctgctcgctc tacgccccaa tgtgcaccga ccaggtctcc
     1681 actcccatcc cagcctgccg gcccatgtgc gagcaggctc gcctgcgctg cgcccccatc
     1741 atggagcaat tcaatttcgg ctggcccgac tcactcgact gtgcccggct ccccacgcgc
     1801 aacgacccgc acgcactctg tatggaggca cccgagaacg ctacagcagg ccccacagaa
     1861 ccccacaagg gcctgggcat gctccctgtg gcacctcggc ccgcgaggcc accgggagat
     1921 tcagccccag gtcccggcag cggtggcacc tgcgacaacc ccgagaagtt ccagtacgtg
     1981 gagaagagtc gctcgtgcgc tccgcgctgc gggccaggcg tcgaggtgtt ctggtctcgg
     2041 cgcgataagg acttcgcgct ggtttggatg gctgtgtggt ccgcgttgtg tttcttctcc
     2101 acggccttca ccgtgttcac cttcctgctg gagcctcacc ggttccagta cccagagcgc
     2161 ccgattatct tcctttctat gtgctacaat gtctactcct tggccttcct gatcagagcg
     2221 gtggcaggtg cacagagtgt ggcatgcgac caggaggcag gggctctgta tgtgatccag
     2281 gagggtctgg aaaacacagg ctgcaccctg gtcttcctgt tgctctatta tttcgggatg
     2341 gccagctcac tttggtgggt agttttgact ctaacctggt tcctggctgc aggcaaaaaa
     2401 tggggccacg aggccatcga ggctcacggc agctacttcc acatggcagc gtggggcctg
     2461 ccagcactca aaactatcgt ggtcctgact ctgcgcaagg tggctggcga tgaactgact
     2521 gggctctgct atgtagccag catggacccg gcagccctca ctggctttgt gttggtaccc
     2581 ctatcttgct acctggtact gggtaccagt ttcctcctga ccggttttgt ggctctcttc
     2641 catatccgca aaatcatgaa gacgggaggc accaatacgg agaagctgga gaagctgatg
     2701 gtcaaaatcg gagtcttttc catcctttac acagtgccgg ccacctgcgt cattgtctgc
     2761 tacgtttatg aacgcctcaa catggacttc tggcggcttc gggccacaga gcaaccatgt
     2821 actgctgcca ccgtgcctgg aggccggaga gactgctcgc tgccaggggg ctcggtgccc
     2881 actgtggctg tcttcatgct caaaatcttc atgtccttgg tggtgggcat caccagtgga
     2941 gtctgggtat ggagttccaa gacttttcag acttggcaga gcctgtgcta ccgaaaaatg
     3001 gcagctggcc gagcccgggc caaggcctgc cgaaccccag ggggctatgg ccggggtacc
     3061 cactgccact acaaagcccc cacggtggtc ttgcacatga ctaagacaga cccctctctg
     3121 gagaacccca cacacctcta gggcgcgcct ctagaactat agtgagtcgt attacgtaga
     3181 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     3241 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     3301 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     3361 gtgggaggtt ttttaattcg cggccgcact cgagatatct agacccagct ttcttgtaca
     3421 aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
     3481 aatgaactag taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga
     3541 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3601 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3661 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3721 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3781 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3841 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3901 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     3961 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4021 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4081 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     4141 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4201 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     4261 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4321 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4381 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     4441 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     4501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4621 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4681 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4741 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     4801 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4861 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4921 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4981 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5401 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     5461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5821 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6121 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6961 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7021 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7081 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7141 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7201 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7261 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7321 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7381 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7441 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7501 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7561 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7621 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     7681 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     7741 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     7801 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7861 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7921 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7981 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8041 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8101 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     8161 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     8221 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     8281 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     8341 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     8401 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     8461 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     8521 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     8581 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     8641 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     8701 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8761 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8821 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8881 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8941 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9001 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9061 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     9121 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     9181 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     9241 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     9301 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     9361 acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg
     9421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc
     9481 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9541 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9661 gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     9721 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     9781 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     9841 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     9901 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     9961 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
    10021 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
    10081 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
    10141 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
    10201 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
    10261 ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca
    10321 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10381 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10441 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
    10501 gcctgc
//
