LOCUS       sequence-199681-       11362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 19 of murine
            Alk and intron 14 of Eml4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno EA
FEATURES             Location/Qualifiers
     source          1..11362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..162
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             214..4314
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             214..4314
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4315..4362
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4385..4401)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4385..4401)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4421..4555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4499..4518
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4698..4720)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      complement(7795..8383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7875..7894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8554..9345,9346..9414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9177..9196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9415..9519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9587..9605
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   9633..9735
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     9823..9973
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        10023..10271
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10293..10368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        10459..10707
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10729..10804
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        10894..11179
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
       61 aatcactttt tttcaggttg gaccggtgcc accatggact ataaggacca cgacggagac
      121 tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag
      181 cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac
      241 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
      301 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
      361 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
      421 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
      481 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
      541 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
      601 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
      661 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
      721 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
      781 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
      841 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
      901 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
      961 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     1021 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     1081 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     1141 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     1201 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     1261 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     1321 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     1381 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     1441 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     1501 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     1561 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     1621 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     1681 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     1741 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     1801 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     1861 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     1921 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     1981 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     2041 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     2101 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     2161 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     2221 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     2281 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     2341 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     2401 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     2461 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     2521 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     2581 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     2641 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     2701 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     2761 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     2821 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     2881 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     2941 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     3001 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     3061 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     3121 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     3181 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     3241 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     3301 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     3361 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     3421 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     3481 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     3541 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     3601 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     3661 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     3721 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     3781 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     3841 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     3901 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     3961 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     4021 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     4081 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     4141 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     4201 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     4261 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg
     4321 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agtaagaatt cctgcagccc
     4381 gggggatcca ctagttctag agcggccgcc accgcgggga gatccagaca tgataagata
     4441 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4501 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4561 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4621 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     4681 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4741 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4801 cggggcgcag ccatgaggtc gactctagtc cccgcggtgg cagatctgga aggtgctgag
     4861 gtacgatgag acccgcacca ggtgcagacc ctgcgagtgt ggcggtaaac atattaggaa
     4921 ccagcctgtg atgctggatg tgaccgagga gctgaggccc gatcacttgg tgctggcctg
     4981 cacccgcgct gagtttggct ctagcgatga agatacagat tgaggtactg aaatgtgtgg
     5041 gcgtggctta agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg
     5101 ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc
     5161 atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag
     5221 cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc
     5281 tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg
     5341 cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag cttcccgttc
     5401 atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga
     5461 acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc
     5521 ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg
     5581 gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga
     5641 ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact
     5701 ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag
     5761 agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg
     5821 gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt
     5881 gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga
     5941 ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag
     6001 aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa
     6061 tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat
     6121 aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac
     6181 gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag
     6241 ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat
     6301 ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa
     6361 gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg
     6421 cgacttaccg cagccggtgg gcccgtaaat cacacctatt accggctgca actggtagtt
     6481 aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct
     6541 gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag
     6601 ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt
     6661 gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc
     6721 tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt
     6781 cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc
     6841 gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg
     6901 aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag
     6961 catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg
     7021 cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc
     7081 gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg
     7141 cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc
     7201 tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa
     7261 aggctgtccg tgtccccgta tacagacttg agaggcctgt cctcgaccga tgcccttgag
     7321 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     7381 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     7441 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     7501 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     7561 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     7621 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     7681 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     7741 gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8221 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8461 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8521 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8581 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8641 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8701 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8761 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8821 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     8881 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8941 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9001 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9061 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9121 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     9181 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9241 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9301 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9361 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9541 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     9601 gaggcccttt cgtcttcaat taattaagct agcatcatca ataatatacc ttattttgga
     9661 ttgaagccaa tatgataatg agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg
     9721 gggcgggtga cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt
     9781 aagcgacgga tgtggcaaaa gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac
     9841 aattttcgcg cggttttagg cggatgttgt agtaaatttg ggcgtaaccg agtaagattt
     9901 ggccattttc gcgggaaaac tgaataagag gaagtgaaat ctgaataatt ttgtgttact
     9961 catagcgcgt aatatttgtc tagggagatc cggtaccgtt taaactcgag aatggcgcat
    10021 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
    10081 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
    10141 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
    10201 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
    10261 gacgaaacac cgtcctggca tgtctatctg tagttttaga gctagaaata gcaagttaaa
    10321 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
    10381 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
    10441 cagacaaatg gctctagaga gggcctattt cccatgattc cttcatattt gcatatacga
    10501 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
    10561 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
    10621 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
    10681 tatatcttgt ggaaaggacg aaacaccgtt tgtcggggtc tactaggagt tttagagcta
    10741 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
    10801 gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc
    10861 gcgtgcgcca attctgcaga caaatggggt acccgttaca taacttacgg taaatggccc
    10921 gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac
    10981 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    11041 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    11101 gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
    11161 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
    11221 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
    11281 gggggcgggg gggggggggg skyggkcbyd tgryksggbg gkgmtsbtbt ytthbgyrct
    11341 tayhgryrcc hcgcsgvccd cy
//
