LOCUS       sequence-199697-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Saraiva LR, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts 
            P, Marioni JC, Logan DW
  TITLE     Hierarchical deconstruction of mouse olfactory sensory neurons: from
            whole mucosa to single-cell RNA-seq.
  JOURNAL   Sci Rep. 2015 Dec 16;5:18178. doi: 10.1038/srep18178.
  PUBMED    26670777
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-16"; volume: "5:18178. doi"; pages: " 
            10.1038/srep18178"
COMMENT     Sequence Label: pISH-Sln
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(616..634)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(617..634)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(652..668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(665..687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    676..692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(700..706,707..724,725..730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    745..766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcgcatgctc ccggccgcca tggccgcggg attgagacag ctggaggtgg agagactgag
       61 gtccttggta gcctgagtgt gcccctgctc ctcttcagga agtgaagaca agccttggtg
      121 tgcaatcaca agtccttctg gagttctcat ccagacattc tgaagatgga gaggtctact
      181 caggagctgt ttatcaactt cacagttgtc ctcatcaccg ttctccttat gtggctcctc
      241 gtgaggtcct accaatactg aggggccatg ctatactcca gggagtgact gctgtgtgcc
      301 ccgagcttcc aatgctctat tgtcatgaga tgctgcttct ggctcctcca gcatctctga
      361 cccacactca caatgcctga cacaccgctg cactaggtcc ttggcatgtt ttctaaagat
      421 gctgttcttg taggctgccc aaagcttgcc agccagtgag ccttagcttt gttccctagc
      481 aaatgtacta ttcaagtcac caggttaaaa ttaaactgga ttcttatgat gcagagtaac
      541 aaatcactag tgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat
      601 gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct
      661 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      721 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      781 actgcccgct ttccagtcgg
//
