LOCUS       sequence-199699-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Drd2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(918..936)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(919..936)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(954..970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(954..970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(967..989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    978..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgctcccggc cgccatggcg gccgcgggaa ttcgattctg tagaggactg gtgggatgtt
       61 gcagtcacag tgtatattca ggatgtgcgt gatgaagaag ggcagccagc agatgatgaa
      121 cacaccaaga acaatggcaa gcatctgagt ggctttcttc tccttctgct gggagagctt
      181 cctgcggctc atcgtcttaa gggaggtccg ggttttgcca ttgggcatgg tctggatctc
      241 aaagaacttg gcaatcctgg gattgacaat cttggcatgc ccattctttt ctggtttggc
      301 aggactgtca gggttgctat gtagaccgtg gtgggatgga tcggggagag tgagctggtg
      361 gtgactggga gggatggggc tataccgggt cctctctggg gggctggtgc ttgacagcat
      421 ctccatttcc agctcctgag ctcggcgggc agcatccatt ctccgcctgt tcactgggaa
      481 actcccatta gacttcatga taacggtgca gagtttcatg tcctcagggt gggtacagtt
      541 gcccttgagt ggtgtcttca ggttggctct gaaagctcgg ctgctacgct tggtgttgac
      601 ccgcttccga cgcttgcgga gaacgatgta gattttgata tagaccagca gggtgacgat
      661 gaagggcacg tagaacgaga cgatggagga gtagaccacg aaggcagggt tggcaatgat
      721 acactcattc tggtctgtgt tgttgagtcc aaagagcagt gggcaagaga tggtgaagga
      781 caggacccag acaatggcga tcatgacagt aactcggcgc ttggagctgt agcgtgtaat
      841 cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg
      901 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      961 ctgtttcctg tgtgaaattg ttatccgctc acaattccac
//
