LOCUS       sequence-199703-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-mCherry
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(162..180)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     430..449
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(609..627)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(610..627)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(645..661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(658..680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    669..685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(693..699,700..717,718..723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    738..759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(876..893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcccggcc gccatggcgg ccgcgggaat tcgattggat aacatggcca tcatcaagga
       61 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      241 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      361 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      421 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      481 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaaaa tcactagtga
      541 attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg gatgcatagc
      601 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
      661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
//
