LOCUS       sequence-199704-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr1031
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(654..672)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(655..672)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(690..706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(690..706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(703..725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    714..730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(738..744,745..762,763..768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    783..804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattttcc tagtaccgta
       61 aatgaggggg ttcaacatgg gaatgacagt ggtatagaac acagcaacca ttttaccttg
      121 ttccacagac tctctagatg ttggtctgag atacatgaaa aaaagagttg catagaatat
      181 gatgacagct gtcagatggg agccacaggt ggagaaagct ttccgcttgc cttctgtaga
      241 gctaatcctc agaatagcag gaaagatata caggtaggaa gtgagaataa tgagcaggga
      301 gaaggagagg ttaaatccag ctacaacaag catggaggtt tctttgtgat aagtgtcaga
      361 acaagccagc ttaatcaatg gtggatcagc acaatagaag tgattgattt cattgtggcc
      421 acagaaagcc aggttgtaag tccacatggt ctccatcaga ccagtaagtg ccccatacac
      481 ataaggcact gtgattagag atgtgcatac actctgggac atcttgctac cataaagcag
      541 agggttgcag attgccacga acctatcaaa cgcaatcact agtgaattcg cggccgcctg
      601 caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag
      661 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
//
