LOCUS       sequence-199713-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr545
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(701..719)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(702..719)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(737..753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(737..753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(750..772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    761..777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(785..791,792..809,810..815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    830..851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atgctcccgg ccgccatggc ggccgcggga attcgattta ccaggtgctg cctgatttcc
       61 ttggttctgg ccccgtagat gatggggttg accagacacg ggagcaacag atagaaagca
      121 ctgagcaggt tgtgcacatc ttgggaggca gtgcgagcca cacggtagac aatggatgag
      181 gacatggtgg aggagtagac ggtgaagatg accagcaggt gggaaccaca ggtgttcagg
      241 gctttggacc gtgctccacc tgatgagatc ctgaaggcag catggatgat gcgggagtag
      301 gaggtgccta gaaggagcat atccaggact ctattaaagg ttcgaataat gagtcccgcc
      361 gttttattca gcgagatgtc tccacaggaa agcttcatca gggccatgtg ctcacaggca
      421 aagtggcgga tcacatctga gcggcagaag cgaacccgcg aggccagcac caccactgga
      481 gcaacaatgc ttgtgctcct ggtgactgcc aacaccacca gaccagccag taactgtcct
      541 gtcactattt ctgggtagcg gagagggtag cagatagcca catagcgatc tagagccatg
      601 accaggagga tgttgcagtc aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      661 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      901 agctgcatta atgaatcggc
//
