LOCUS       sequence-199717-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr819
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(651..669)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(652..669)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(687..703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(700..722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    711..727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(735..741,742..759,760..765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    780..801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(918..935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 catgctcccg gccgccatgg cggccgcggg aattcgattt gctccacaaa agggcagagt
       61 gaagatccag gtggtctggg ctgattctac caggatccca gtcatccaag atgcaccagc
      121 caacttcaga cagacagaag ggtccatccg cagagtgtaa tgtagtgggt aacaaatagc
      181 cacaaagcga tcataagcca tagttgccag aaggcaacac tctgtcagcc ccaagatggt
      241 gaatacatac atctgagctg cacagcctgc cacacttatg gtcttgagct gtaccagcag
      301 gtgcaccagc atctgaggca ccacactgct gatatagcac atctctagga aggagaggtt
      361 ggccaggaag aagtacatgg gcgtgtgtag agaaggtgtg gtccagatca cacagatgat
      421 ggtgaggttt cctcccactg taaacaagta gagaaccaag aacacaacaa agaggacaag
      481 ctgcagctct gctagagaag aaaaggccac caatgtgaat tcagtgcaga gggacaggct
      541 tccattcttc atgagctcag agttagacat aatcactagt gaattcgcgg ccgcctgcag
      601 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
      661 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
//
