LOCUS       sequence-199719-        5716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP in the presence of ipgC and mxiE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  TITLE     Unpublished Murray Lab Plasmids
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PipaH-deGFP
FEATURES             Location/Qualifiers
     source          1..5716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             366..388
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(425..446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(686..705)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    1130..1150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1555..2349)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1666..1685)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2276..2295
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      3829..4600
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             4601..5263
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 gatccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
       61 aggctagggt caaaaatcgt ggcgttgaca aaatggctgc gttacgtcat tgagcatatc
      121 caggactggc cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga tctgacctct
      181 cagtaaatat caatacggtt cttacgagcc gcttaccgtt caaatatgaa gtacgatgtt
      241 taactaaccg aaaaacaaga acaatacggt gcaaacaggc cattcacggt taactgaaac
      301 agtatcgttt ttttacagcc aattttgttt atccttatta agataaaaaa gtgctaagca
      361 ataattttgt ttaactttaa gaaggagata tacaatggag cttttcactg gcgttgttcc
      421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      481 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      541 gcccgtgccc tggcccaccc tcgttaccac cctgacctac ggcgtgcagt gcttcagccg
      601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      841 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      901 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      961 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1021 gaagcgcgat cacatggtca tgctggagtt cgtgaccgcc gccgggatct aactcgagca
     1081 aagcccgccg aaaggcgggc ttttctgtgt cgaccgatgc ccttactagt gagtgagcta
     1141 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1201 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatgca
     1261 taaaaactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1321 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1381 gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     1441 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     1501 gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
     1561 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     1621 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     1681 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     1741 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     1801 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     1861 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     1921 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     1981 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     2041 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     2101 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     2161 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     2221 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     2281 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     2341 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gattttgatc ccctgcgcca
     2401 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     2461 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     2521 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     2581 tgtccagata gcccagtagc tgacattcat cccaggtggc acttttcggg gaaatgtgcg
     2641 cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc
     2701 agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat atcccgattc
     2761 aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg ggccgtctct
     2821 tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg tagggcaggc
     2881 tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg cgtctcaacg
     2941 cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg tggctcgacc
     3001 gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc gtagaacgcc
     3061 tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc tagatcggcc
     3121 acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgtttcc gatgaactcc
     3181 ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat gtgcgggctg
     3241 gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt gtccgccagc
     3301 cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga cttccaccat
     3361 tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg ccgcttctct
     3421 ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc ccagtgctcg
     3481 ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta ggcgtgcttg
     3541 agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat cgcgtatgcc
     3601 gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc gcaaggcggt
     3661 gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc gcaaagtcgt
     3721 gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg ccctgcgcgg
     3781 tcgctgcgct cccttgccag cccatggata tgtggacgat ggccgcgagc ggccaccggc
     3841 tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga caggctgcgc
     3901 ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg accacatacc
     3961 caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa ttttctctgg
     4021 ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc agttggacac caagtggaag
     4081 gcgggtcaag gctcgcgcag cgaccgcgca gcgtcttggc cttgacgcgc ctggaacgac
     4141 ccaagcctat gcgagtgggg gcagtcgaag ggcgaagccc gcccgcctgc cccccgagcc
     4201 tcacggcggc gagtgcgggg gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc
     4261 cgcgcagtcg atcaacaagc cccggagggg ccactttttg ccggaggggg agccgcgccg
     4321 aaggcgtggg ggaaccccgc aggggtgccc ttctttgggc accaaagaac tagatatagg
     4381 gcgaaatgcg aaagacttaa aaatcaacaa cttaaaaaag gggggtacgc aacagctcat
     4441 tgcggcaccc cccgcaatag ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca
     4501 cttttacgca acgcataatt gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg
     4561 tgccgcaacc gtgcggcacc cctaccgcat ggagataagc atggccacgc agtccagaga
     4621 aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac gcaaagcgca
     4681 tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc tgcatcacct
     4741 cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac tttccaagct
     4801 catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg ccgagcgctg
     4861 gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg tcaatgaccg
     4921 cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg ccgccgtggt
     4981 ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc gccgcatccc
     5041 gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc cgcccagcca
     5101 gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg aggaatggga
     5161 acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg acgatggcga
     5221 gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcacttgg gttgcgcagc
     5281 aacccgtaag tgcgctgttc cagactatcg gctgtagccg cctcgccgcc ctataccttg
     5341 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
     5401 ccggcggcac ctcgctaacg gattcaccgt ttttatcagg ctctgggagg cagaataaat
     5461 gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt
     5521 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     5581 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     5641 tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg
     5701 caccaataac tgcctg
//
