LOCUS       sequence-199721-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Pde4a
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      813..822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(856..875)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(857..875)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(882..899)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(882..898)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgacctgca ggcggccgcg aattcactag tgattagcct ctgtggagaa gtctcaggtg
       61 ggcttcatcg actatattgt tcacccactg tgggagacgt gggccgatct cgtccacccc
      121 gatgcccaag acatcctgga cacgctggaa gacaaccggg attggtatca cagtgccatc
      181 cgccagagtc cctccccaac cctggaagag gagccaggag tgctcagcga tccggccctg
      241 cctgacaagt tccaatttga gctcaccttg gaggaagagg atgaagagga ttcattggag
      301 gttccaggat tgccttgcac tgaggaaact ctcctggctc cacatgatac cagagctcaa
      361 gccatggaac agtcaaaggt caaaggccag agccctgctg ttgtggaggt agcagagagt
      421 ttgaagcagg agacagcctc agcacatggc gctcctgagg agtccgcgga ggctgtgggc
      481 cattccttca gccttgaaac ctctattctg cctgacttga ggaccctgtc cccctcagag
      541 gaggcccagg gcctcctggg cctcccctcc atggcggcag aggtggaggc cccaagagac
      601 catctggctg ccatgagggc ttgttctgcc tgctcgggga catcaggaga caattctgcc
      661 gtcatctctg ctccaggcag gtgggggtca ggtggagacc ctgcctgatc ccttttccct
      721 tcaccctcgg ctcctccctc ctccccacac tctccactca ccacagacca cctcaacgac
      781 tcctggccct cctgagaaat cgaattcccg cggccgccat ggcggccggg agcatgcgac
      841 gtcgggccca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
//
