LOCUS       sequence-199727-       16409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Wang SC, Shao R, Pao AY, Zhang S, Hung MC, Su LK
  TITLE     Inhibition of cancer cell growth by BRCA2.
  JOURNAL   Cancer Res. 2002 Mar 1. 62(5):1311-4.
  PUBMED    11888897
REFERENCE   2  (bases 1 to 16409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2002 Mar 1. 62(5):1311-4."
COMMENT     Sequence Label: pCIN BRCA2 WT
FEATURES             Location/Qualifiers
     source          1..16409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    38..159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(75..94)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     129..148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(208..224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(221..243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(256..262,263..280,281..286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    301..322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(439..456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(610..1198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(690..709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1369..2160,2161..2229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1992..2011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2230..2334)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2409..2428)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2600..2979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2980..3183
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3134..3154
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3228..3247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3228..3246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          3283..3855
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(14327..14345)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(14328..14345)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(14365..14382)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14371..14595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14641..15069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14728..14747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14938..14959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(15078..15098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        15083..15412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15263..15398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15325..15344
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             15479..16273
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15533..15552)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16143..16162
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
       61 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
      181 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2341 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     2401 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     2461 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     2521 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     2581 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2641 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2701 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2761 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2821 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2881 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2941 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3001 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3061 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3121 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3181 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     3241 tatagggaga cccaagcttc gaccgatcct gagaacttca gggtgagttt ggggaccctt
     3301 gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc
     3361 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
     3421 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
     3481 tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt
     3541 gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt
     3601 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     3661 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
     3721 tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
     3781 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
     3841 ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc
     3901 aaagaattcc tcgacggatc cactagtaac ggccgccagt gtgctggaat tctgcagata
     3961 tccatcacac tggcggccgc tcgaggtaaa aatgcctatt ggatccaaag agaggccaac
     4021 attttttgaa atttttaaga cacgctgcaa caaagcagat ttaggaccaa taagtcttaa
     4081 ttggtttgaa gaactttctt cagaagctcc accctataat tctgaacctg cagaagaatc
     4141 tgaacataaa aacaacaatt acgaaccaaa cctatttaaa actccacaaa ggaaaccatc
     4201 ttataatcag ctggcttcaa ctccaataat attcaaagag caagggctga ctctgccgct
     4261 gtaccaatct cctgtaaaag aattagataa attcaaatta gacttaggaa ggaatgttcc
     4321 caatagtaga cataaaagtc ttcgcacagt gaaaactaaa atggatcaag cagatgatgt
     4381 ttcctgtcca cttctaaatt cttgtcttag tgaaagtcct gttgttctac aatgtacaca
     4441 tgtaacacca caaagagata agtcagtggt atgtgggagt ttgtttcata caccaaagtt
     4501 tgtgaagggt cgtcagacac caaaacatat ttctgaaagt ctaggagctg aggtggatcc
     4561 tgatatgtct tggtcaagtt ctttagctac accacccacc cttagttcta ctgtgctcat
     4621 agtcagaaat gaagaagcat ctgaaactgt atttcctcat gatactactg ctaatgtgaa
     4681 aagctatttt tccaatcatg atgaaagtct gaagaaaaat gatagattta tcgcttctgt
     4741 gacagacagt gaaaacacaa atcaaagaga agctgcaagt catggatttg gaaaaacatc
     4801 agggaattca tttaaagtaa atagctgcaa agaccacatt ggaaagtcaa tgccaaatgt
     4861 cctagaagat gaagtatatg aaacagttgt agatacctct gaagaagata gtttttcatt
     4921 atgtttttct aaatgtagaa caaaaaatct acaaaaagta agaactagca agactaggaa
     4981 aaaaattttc catgaagcaa acgctgatga atgtgaaaaa tctaaaaacc aagtgaaaga
     5041 aaaatactca tttgtatctg aagtggaacc aaatgatact gatccattag attcaaatgt
     5101 agcaaatcag aagccctttg agagtggaag tgacaaaatc tccaaggaag ttgtaccgtc
     5161 tttggcctgt gaatggtctc aactaaccct ttcaggtcta aatggagccc agatggagaa
     5221 aataccccta ttgcatattt cttcatgtga ccaaaatatt tcagaaaaag acctattaga
     5281 cacagagaac aaaagaaaga aagattttct tacttcagag aattctttgc cacgtatttc
     5341 tagcctacca aaatcagaga agccattaaa tgaggaaaca gtggtaaata agagagatga
     5401 agagcagcat cttgaatctc atacagactg cattcttgca gtaaagcagg caatatctgg
     5461 aacttctcca gtggcttctt catttcaggg tatcaaaaag tctatattca gaataagaga
     5521 atcacctaaa gagactttca atgcaagttt ttcaggtcat atgactgatc caaactttaa
     5581 aaaagaaact gaagcctctg aaagtggact ggaaatacat actgtttgct cacagaagga
     5641 ggactcctta tgtccaaatt taattgataa tggaagctgg ccagccacca ccacacagaa
     5701 ttctgtagct ttgaagaatg caggtttaat atccactttg aaaaagaaaa caaataagtt
     5761 tatttatgct atacatgatg aaacatctta taaaggaaaa aaaataccga aagaccaaaa
     5821 atcagaacta attaactgtt cagcccagtt tgaagcaaat gcttttgaag caccacttac
     5881 atttgcaaat gctgattcag gtttattgca ttcttctgtg aaaagaagct gttcacagaa
     5941 tgattctgaa gaaccaactt tgtccttaac tagctctttt gggacaattc tgaggaaatg
     6001 ttctagaaat gaaacatgtt ctaataatac agtaatctct caggatcttg attataaaga
     6061 agcaaaatgt aataaggaaa aactacagtt atttattacc ccagaagctg attctctgtc
     6121 atgcctgcag gaaggacagt gtgaaaatga tccaaaaagc aaaaaagttt cagatataaa
     6181 agaagaggtc ttggctgcag catgtcaccc agtacaacat tcaaaagtgg aatacagtga
     6241 tactgacttt caatcccaga aaagtctttt atatgatcat gaaaatgcca gcactcttat
     6301 tttaactcct acttccaagg atgttctgtc aaacctagtc atgatttcta gaggcaaaga
     6361 atcatacaaa atgtcagaca agcttaaagg taacaattat gaatctgatg ttgaattaac
     6421 caaaaatatt cccatggaaa agaatcaaga tgtatgtgct ttaaatgaaa attataaaaa
     6481 cgttgagctg ttgccacctg aaaaatacat gagagtagca tcaccttcaa gaaaggtaca
     6541 attcaaccaa aacacaaatc taagagtaat ccaaaaaaat caagaagaaa ctacttcaat
     6601 ttcaaaaata actgtcaatc cagactctga agaacttttc tcagacaatg agaataattt
     6661 tgtcttccaa gtagctaatg aaaggaataa tcttgcttta ggaaatacta aggaacttca
     6721 tgaaacagac ttgacttgtg taaacgaacc cattttcaag aactctacca tggttttata
     6781 tggagacaca ggtgataaac aagcaaccca agtgtcaatt aaaaaagatt tggtttatgt
     6841 tcttgcagag gagaacaaaa atagtgtaaa gcagcatata aaaatgactc taggtcaaga
     6901 tttaaaatcg gacatctcct tgaatataga taaaatacca gaaaaaaata atgattacat
     6961 gaacaaatgg gcaggactct taggtccaat ttcaaatcac agttttggag gtagcttcag
     7021 aacagcttca aataaggaaa tcaagctctc tgaacataac attaagaaga gcaaaatgtt
     7081 cttcaaagat attgaagaac aatatcctac tagtttagct tgtgttgaaa ttgtaaatac
     7141 cttggcatta gataatcaaa agaaactgag caagcctcag tcaattaata ctgtatctgc
     7201 acatttacag agtagtgtag ttgtttctga ttgtaaaaat agtcatataa cccctcagat
     7261 gttattttcc aagcaggatt ttaattcaaa ccataattta acacctagcc aaaaggcaga
     7321 aattacagaa ctttctacta tattagaaga atcaggaagt cagtttgaat ttactcagtt
     7381 tagaaagcca agctacatat tgcagaagag tacatttgaa gtgcctgaaa accagatgac
     7441 tatcttaaag accacttctg aggaatgcag agatgctgat cttcatgtca taatgaatgc
     7501 cccatcgatt ggtcaggtag acagcagcaa gcaatttgaa ggtacagttg aaattaaacg
     7561 gaagtttgct ggcctgttga aaaatgactg taacaaaagt gcttctggtt atttaacaga
     7621 tgaaaatgaa gtggggttta ggggctttta ttctgctcat ggcacaaaac tgaatgtttc
     7681 tactgaagct ctgcaaaaag ctgtgaaact gtttagtgat attgagaata ttagtgagga
     7741 aacttctgca gaggtacatc caataagttt atcttcaagt aaatgtcatg attctgttgt
     7801 ttcaatgttt aagatagaaa atcataatga taaaactgta agtgaaaaaa ataataaatg
     7861 ccaactgata ttacaaaata atattgaaat gactactggc acttttgttg aagaaattac
     7921 tgaaaattac aagagaaata ctgaaaatga agataacaaa tatactgctg ccagtagaaa
     7981 ttctcataac ttagaatttg atggcagtga ttcaagtaaa aatgatactg tttgtattca
     8041 taaagatgaa acggacttgc tatttactga tcagcacaac atatgtctta aattatctgg
     8101 ccagtttatg aaggagggaa acactcagat taaagaagat ttgtcagatt taactttttt
     8161 ggaagttgcg aaagctcaag aagcatgtca tggtaatact tcaaataaag aacagttaac
     8221 tgctactaaa acggagcaaa atataaaaga ttttgagact tctgatacat tttttcagac
     8281 tgcaagtggg aaaaatatta gtgtcgccaa agagtcattt aataaaattg taaatttctt
     8341 tgatcagaaa ccagaagaat tgcataactt ttccttaaat tctgaattac attctgacat
     8401 aagaaagaac aaaatggaca ttctaagtta tgaggaaaca gacatagtta aacacaaaat
     8461 actgaaagaa agtgtcccag ttggtactgg aaatcaacta gtgaccttcc agggacaacc
     8521 cgaacgtgat gaaaagatca aagaacctac tctgttgggt tttcatacag ctagcgggaa
     8581 aaaagttaaa attgcaaagg aatctttgga caaagtgaaa aacctttttg atgaaaaaga
     8641 gcaaggtact agtgaaatca ccagttttag ccatcaatgg gcaaagaccc taaagtacag
     8701 agaggcctgt aaagaccttg aattagcatg tgagaccatt gagatcacag ctgccccaaa
     8761 gtgtaaagaa atgcagaatt ctctcaataa tgataaaaac cttgtttcta ttgagactgt
     8821 ggtgccacct aagctcttaa gtgataattt atgtagacaa actgaaaatc tcaaaacatc
     8881 aaaaagtatc tttttgaaag ttaaagtaca tgaaaatgta gaaaaagaaa cagcaaaaag
     8941 tcctgcaact tgttacacaa atcagtcccc ttattcagtc attgaaaatt cagccttagc
     9001 tttttacaca agttgtagta gaaaaacttc tgtgagtcag acttcattac ttgaagcaaa
     9061 aaaatggctt agagaaggaa tatttgatgg tcaaccagaa agaataaata ctgcagatta
     9121 tgtaggaaat tatttgtatg aaaataattc aaacagtact atagctgaaa atgacaaaaa
     9181 tcatctctcc gaaaaacaag atacttattt aagtaacagt agcatgtcta acagctattc
     9241 ctaccattct gatgaggtat ataatgattc aggatatctc tcaaaaaata aacttgattc
     9301 tggtattgag ccagtattga agaatgttga agatcaaaaa aacactagtt tttccaaagt
     9361 aatatccaat gtaaaagatg caaatgcata cccacaaact gtaaatgaag atatttgcgt
     9421 tgaggaactt gtgactagct cttcaccctg caaaaataaa aatgcagcca ttaaattgtc
     9481 catatctaat agtaataatt ttgaggtagg gccacctgca tttaggatag ccagtggtaa
     9541 aatcgtttgt gtttcacatg aaacaattaa aaaagtgaaa gacatattta cagacagttt
     9601 cagtaaagta attaaggaaa acaacgagaa taaatcaaaa atttgccaaa cgaaaattat
     9661 ggcaggttgt tacgaggcat tggatgattc agaggatatt cttcataact ctctagataa
     9721 tgatgaatgt agcacgcatt cacataaggt ttttgctgac attcagagtg aagaaatttt
     9781 acaacataac caaaatatgt ctggattgga gaaagtttct aaaatatcac cttgtgatgt
     9841 tagtttggaa acttcagata tatgtaaatg tagtataggg aagcttcata agtcagtctc
     9901 atctgcaaat acttgtggga tttttagcac agcaagtgga aaatctgtcc aggtatcaga
     9961 tgcttcatta caaaacgcaa gacaagtgtt ttctgaaata gaagatagta ccaagcaagt
    10021 cttttccaaa gtattgttta aaagtaacga acattcagac cagctcacaa gagaagaaaa
    10081 tactgctata cgtactccag aacatttaat atcccaaaaa ggcttttcat ataatgtggt
    10141 aaattcatct gctttctctg gatttagtac agcaagtgga aagcaagttt ccattttaga
    10201 aagttcctta cacaaagtta agggagtgtt agaggaattt gatttaatca gaactgagca
    10261 tagtcttcac tattcaccta cgtctagaca aaatgtatca aaaatacttc ctcgtgttga
    10321 taagagaaac ccagagcact gtgtaaactc agaaatggaa aaaacctgca gtaaagaatt
    10381 taaattatca aataacttaa atgttgaagg tggttcttca gaaaataatc actctattaa
    10441 agtttctcca tatctctctc aatttcaaca agacaaacaa cagttggtat taggaaccaa
    10501 agtctcactt gttgagaaca ttcatgtttt gggaaaagaa caggcttcac ctaaaaacgt
    10561 aaaaatggaa attggtaaaa ctgaaacttt ttctgatgtt cctgtgaaaa caaatataga
    10621 agtttgttct acttactcca aagattcaga aaactacttt gaaacagaag cagtagaaat
    10681 tgctaaagct tttatggaag atgatgaact gacagattct aaactgccaa gtcatgccac
    10741 acattctctt tttacatgtc ccgaaaatga ggaaatggtt ttgtcaaatt caagaattgg
    10801 aaaaagaaga ggagagcccc ttatcttagt gggagaaccc tcaatcaaaa gaaacttatt
    10861 aaatgaattt gacaggataa tagaaaatca agaaaaatcc ttaaaggctt caaaaagcac
    10921 tccagatggc acaataaaag atcgaagatt gtttatgcat catgtttctt tagagccgat
    10981 tacctgtgta ccctttcgca caactaagga acgtcaagag atacagaatc caaattttac
    11041 cgcacctggt caagaatttc tgtctaaatc tcatttgtat gaacatctga ctttggaaaa
    11101 atcttcaagc aatttagcag tttcaggaca tccattttat caagtttctg ctacaagaaa
    11161 tgaaaaaatg agacacttga ttactacagg cagaccaacc aaagtctttg ttccaccttt
    11221 taaaactaaa tcacattttc acagagttga acagtgtgtt aggaatatta acttggagga
    11281 aaacagacaa aagcaaaaca ttgatggaca tggctctgat gatagtaaaa ataagattaa
    11341 tgacaatgag attcatcagt ttaacaaaaa caactccaat caagcagcag ctgtaacttt
    11401 cacaaagtgt gaagaagaac ctttagattt aattacaagt cttcagaatg ccagagatat
    11461 acaggatatg cgaattaaga agaaacaaag gcaacgcgtc tttccacagc caggcagtct
    11521 gtatcttgca aaaacatcca ctctgcctcg aatctctctg aaagcagcag taggaggcca
    11581 agttccctct gcgtgttctc ataaacagct gtatacgtat ggcgtttcta aacattgcat
    11641 aaaaattaac agcaaaaatg cagagtcttt tcagtttcac actgaagatt attttggtaa
    11701 ggaaagttta tggactggaa aaggaataca gttggctgat ggcggatggc tcataccctc
    11761 caatgatgga aaggctggaa aagaagaatt ttatagggct ctgtgtgaca ctccaggtgt
    11821 ggatccaaag cttatttcta gaatttgggt ttataatcac tatagatgga tcatatggaa
    11881 actggcagct atggaatgtg cctttcctaa ggaatttgct aatagatgcc taagcccaga
    11941 aagggtgctt cttcaactaa aatacagata tgatacggaa attgatagaa gcagaagatc
    12001 ggctataaaa aagataatgg aaagggatga cacagctgca aaaacacttg ttctctgtgt
    12061 ttctgacata atttcattga gcgcaaatat atctgaaact tctagcaata aaactagtag
    12121 tgcagatacc caaaaagtgg ccattattga acttacagat gggtggtatg ctgttaaggc
    12181 ccagttagat cctcccctct tagctgtctt aaagaatggc agactgacag ttggtcagaa
    12241 gattattctt catggagcag aactggtggg ctctcctgat gcctgtacac ctcttgaagc
    12301 cccagaatct cttatgttaa agatttctgc taacagtact cggcctgctc gctggtatac
    12361 caaacttggg ttctttcctg accctagacc ttttcctctg cccttatcat cgcttttcag
    12421 tgatggagga aatgttggtt gtgttgatgt aattattcaa agagcatacc ctatacagtg
    12481 gatggagaag acatcatctg gattatacat atttcgcaat gaaagagagg aagaaaagga
    12541 agcagcaaaa tatgtggagg cccaacaaaa gagactagaa gccttattca ctaaaattca
    12601 ggaggaattt gaagaacatg aagaaaacac aacaaaacca tatttaccat cacgtgcact
    12661 aacaagacag caagttcgtg ctttgcaaga tggtgcagag ctttatgaag cagtgaagaa
    12721 tgcagcagac ccagcttacc ttgagggtta tttcagtgaa gagcagttaa gagccttgaa
    12781 taatcacagg caaatgttga atgataagaa acaagctcag atccagttgg aaattaggaa
    12841 ggccatggaa tctgctgaac aaaaggaaca aggtttatca agggatgtca caaccgtgtg
    12901 gaagttgcgt attgtaagct attcaaaaaa agaaaaagat tcagttatac tgagtatttg
    12961 gcgtccatca tcagatttat attctctgtt aacagaagga aagagataca gaatttatca
    13021 tcttgcaact tcaaaatcta aaagtaaatc tgaaagagct aacatacagt tagcagcgac
    13081 aaaaaaaact cagtatcaac aactaccggt ttcagatgaa attttatttc agatttacca
    13141 gccacgggag ccccttcact tcagcaaatt tttagatcca gactttcagc catcttgttc
    13201 tgaggtggac ctaataggat ttgtcgtttc tgttgtgaaa aaaacaggac ttgccccttt
    13261 cgtctatttg tcagacgaat gttacaattt actggcaata aagttttgga tagaccttaa
    13321 tgaggacatt attaagcctc atatgttaat tgctgcaagc aacctccagt ggcgaccaga
    13381 atccaaatca ggccttctta ctttatttgc tggagatttt tctgtgtttt ctgctagtcc
    13441 aaaagagggc cactttcaag agacattcaa caaaatgaaa aatactgttg agaatattga
    13501 catactttgc aatgaagcag aaaacaagct tatgcatata ctgcatgcaa atgatcccaa
    13561 gtggtccacc ccaactaaag actgtacttc agggccgtac actgctcaaa tcattcctgg
    13621 tacaggaaac aagcttctga tgtcttctcc taattgtgag atatattatc aaagtccttt
    13681 atcactttgt atggccaaaa ggaagtctgt ttccacacct gtctcagccc agatgacttc
    13741 aaagtcttgt aaaggggaga aagagattga tgaccaaaag aactgcaaaa agagaagagc
    13801 cttggatttc ttgagtagac tgcctttacc tccacctgtt agtcccattt gtacatttgt
    13861 ttctccggct gcacagaagg catttcagcc accaaggagt tgtggcacca aatacgaaac
    13921 acccataaag aaaaaagaac tgaattctcc tcagatgact ccatttaaaa aattcaatga
    13981 aatttctctt ttggaaagta attcaatagc tgacgaagaa cttgcattga taaataccca
    14041 agctcttttg tctggttcaa caggagaaaa acaatttata tctgtcagtg aatccactag
    14101 gactgctccc accagttcag aagattatct cagactgaaa cgacgttgta ctacatctct
    14161 gatcaaagaa caggagagtt cccaggccag tacggaagaa tgtgagaaaa ataagcagga
    14221 cacaattaca actaaaaaat atatctaagc atttgcaaag gcgacaataa attattgacg
    14281 cttaaccttt ccagtttatc tcgagcatgc atctagaggg ccctattcta tagtgtcacc
    14341 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
    14401 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
    14461 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
    14521 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
    14581 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
    14641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    14701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    14761 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    14821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    14881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    14941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    15001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    15061 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    15121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    15181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    15241 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    15301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
    15361 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    15421 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
    15481 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    15541 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    15601 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
    15661 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    15721 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    15781 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    15841 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    15901 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    15961 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
    16021 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    16081 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
    16141 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    16201 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    16261 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
    16321 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    16381 ttccgggacg ccggctggat gatcctcca
//
